Zinc ions regulate opening of tight junction favouring efflux of macromoleculesviathe GSK3β/snail-mediated pathway

Metallomics ◽  
2018 ◽  
Vol 10 (1) ◽  
pp. 169-179 ◽  
Author(s):  
Ruyue Xiao ◽  
Lan Yuan ◽  
Weijiang He ◽  
Xiaoda Yang
Keyword(s):  
Tight Junction ◽  
Mdck Cell ◽  
Cell Monolayer ◽  
Zinc Ions

Zn2+-Induced asymmetric paracellular pore paths in MDCK cell monolayer favour efflux of macromoleculesviathe GSK3β/snail-mediated pathway.

Exact kinetic analysis of passive transport across a polarized confluent MDCK cell monolayer modeled as a single barrier

2004 ◽  
Vol 93 (8) ◽  
pp. 2108-2123 ◽  
Author(s):  
Thuy Thanh Tran ◽  
Tracy Gales ◽  
Beverly Maleeff ◽  
Tanya Aldinger ◽  
Joseph W. Polli ◽  
...  
Keyword(s):  
Kinetic Analysis ◽  
Mdck Cell ◽  
Cell Monolayer ◽  
Passive Transport

HGF regulates tight junctions in new nontumorigenic gastric epithelial cell line

2001 ◽  
Vol 280 (5) ◽  
pp. G910-G921 ◽  
Author(s):  
Frederic Hollande ◽  
Emmanuelle M. Blanc ◽  
Jean Pierre Bali ◽  
Robert H. Whitehead ◽  
Andre Pelegrin ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Epithelial Cell ◽  
Tight Junction ◽  
Cell Line ◽  
Cell Monolayer ◽  
Gastric Epithelial Cell ◽  
Epithelial Cell Line ◽  
Dependent Manner ◽  
Membrane Expression ◽  
Epithelial Cell Differentiation

The regulation of intercellular adhesion by hepatocyte growth factor (HGF) was examined on a novel nontumorigenic gastric epithelial cell line (IMGE-5) derived from H-2Kb-tsA58 transgenic mice. IMGE-5 cells constitutively expressed cytokeratin 18 and HGF receptors. Under permissive conditions (33°C + interferon-γ), IMGE-5 cells proliferated rapidly but did not display membrane expression of adherens and tight junction proteins. Under nonpermissive conditions, their proliferation was decreased and they displayed a strong, localized membrane expression of E-cadherin/β-catenin and occludin/ZO-1. HGF treatment largely prevented the targeting of ZO-1 to the tight junction and induced a significant decrease of the transepithelial resistance measured across a confluent IMGE-5 cell monolayer. HGF rapidly increased the tyrosine phosphorylation of ZO-1 and decreased its association with occludin in a phosphatidylinositol 3-kinase (PI 3-kinase)-dependent manner. PI 3-kinase was also involved in HGF-induced migration of IMGE-5 cells. Our results demonstrate that 1) HGF prevents the appearance of ZO-1 in the membrane during epithelial cell differentiation; 2) HGF causes partial relocalization of ZO-1 to the cytoplasm and nucleus and concomitantly stimulates cell dissociation and migration; and 3) IMGE-5 cells offer a useful model for the study of gastric epithelial cell differentiation.

Stabilization of the Tight Junction of the Intestinal Caco-2 Cell Monolayer by Milk Whey Proteins

1995 ◽  
Vol 59 (10) ◽  
pp. 1951-1952 ◽  
Author(s):  
Kei Hashimoto ◽  
Kyoko Takeda ◽  
Tsutomu Nakayama ◽  
Makoto Shimizu
Keyword(s):  
Tight Junction ◽  
Cell Monolayer ◽  
Whey Proteins ◽  
Milk Whey

Hepatocyte growth factor induces MDCK cell morphogenesis without causing loss of tight junction functional integrity

2004 ◽  
Vol 286 (3) ◽  
pp. C482-C494 ◽  
Author(s):  
Anne L. Pollack ◽  
Gerard Apodaca ◽  
Keith E. Mostov
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Tight Junction ◽  
Mdck Cell ◽  
Mdck Cells ◽  
Peak Effect ◽  
Ruthenium Red ◽  
Functional Integrity ◽  
Organ Regeneration ◽  
Hepatocyte Growth

Hepatocyte growth factor (HGF) induces mitogenesis, motogenesis, and tubulogenesis of cultured Madin-Darby canine kidney (MDCK) epithelial cells. We report that in addition to these effects HGF stimulates morphogenesis of tight, polarized MDCK cell monolayers into pseudostratified layers without loss of tight junction (TJ) functional integrity. We tested TJ functional integrity during formation of pseudostratified layers. In response to HGF, the TJ marker ZO-1 remained in morphologically complete rings and functional barriers to paracellular diffusion of ruthenium red were maintained in pseudostratified layers. Transepithelial resistance (TER) increased transiently two- to threefold during the morphogenetic transition from monolayers to pseudostratified layers and then declined to baseline levels once pseudostratified layers were formed. In MDCK cells expressing the trk/met chimera, both HGF and NGF at concentrations of 2.5 ng/ml induced scattering. However, 2.5 ng/ml HGF did not affect TER. The peak effect of HGF on TER was at a concentration of 100 ng/ml. In contrast, NGF at concentrations as high as 25 μg/ml had no effect on TER or pseudostratified layer morphogenesis of trk/met-expressing cultures. These results suggest that altered presentation of the stimulus, such as through HGF interaction with low-affinity sites, may change the downstream signaling response. In addition, our results demonstrate that HGF stimulates pseudostratified layer morphogenesis while inducing an increase in TER and maintaining the overall tightness of the epithelial layer. Stimulation of epithelial cell movements by HGF without loss of functional TJs may be important for maintaining epithelial integrity during morphogenetic events such as formation of pseudostratified epithelia, organ regeneration, and tissue repair.

Synthesis and phosphorylation of cytoskeletal proteins during the in vitro biogenesis of MDCK cell monolayers

1989 ◽  
Vol 93 (1) ◽  
pp. 53-61
Author(s):  
G. Benitez-King ◽  
F. Cazares ◽  
I. Meza
Keyword(s):  
Amino Acid ◽  
Mdck Cell ◽  
Cell Shape ◽  
Cell Monolayer ◽  
Amino Acid Uptake ◽  
Culture Conditions ◽  
Cytoskeletal Proteins ◽  
Acid Uptake ◽  
Suspended Cells

In vitro formation of a functional MDCK cell monolayer requires the dynamic participation of the cytoskeleton. Cell shape, contacts and polarity, as well as transepithelial electric resistance (TER), are actively modified during this differentiation process. We studied the distribution and rearrangement of cytokeratin, vimentin and actin filaments that occur in the monolayer concomitant with the synthesis and phosphorylation of these proteins. Cells cultured for short time in suspension, subconfluent or early confluent cells, showed striking differences in cell shape and arrangement of their cytoskeletal filaments. Subconfluent and early confluent cells synthesized proteins at high levels. In contrast, suspended cells maintained lower rates of protein synthesis. Labeled amino acid uptake was very similar in all these culture conditions. Gel electrophoresis analyses of the synthesized proteins showed increases in the synthesis of actin, vimentin and specific cytokeratins in subconfluent and early confluent cells. On the other hand a decrease in total protein, actin, vimentin and cytokeratin synthesis was observed in cells kept in suspension for 24 h or in 78-h or 174-h older confluent cultures. These cultures also showed a decrease in the uptake of labeled amino acid. Cytokeratin and vimentin phosphorylation rates were also modified during the in vitro formation of a monolayer. In suspended cells, cytokeratins were phosphorylated and three labeled isoelectric variants of the 40, 48 and 58K (K = 10(3) Mr) cytokeratins were present in intermediate filament extracts. In subconfluent and early confluent cells only two isoelectric phosphorylated variants of the 40, 48 and 58K cytokeratins were detected and vimentin was also phosphorylated.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Penetration of Salmonella through a polarized Madin-Darby canine kidney epithelial cell monolayer.

1988 ◽  
Vol 107 (1) ◽  
pp. 221-230 ◽  
Author(s):  
B B Finlay ◽  
B Gumbiner ◽  
S Falkow
Keyword(s):  
Mdck Cell ◽  
Pathogenic Bacteria ◽  
Mdck Cells ◽  
Cell Monolayer ◽  
Apical Surface ◽  
Madin Darby Canine Kidney ◽  
Intracellular Parasites ◽  
Epithelial Barriers ◽  
Darby Canine Kidney ◽  
Canine Kidney

Many intracellular parasites are capable of penetrating host epithelial barriers. To study this process in more detail we examined the interactions between the pathogenic bacteria Salmonella choleraesuis and polarized epithelial monolayers of Madin-Darby canine kidney (MDCK) cells grown on membrane filters. Association of bacteria with the MDCK cell apical surface was an active event, requiring bacterial RNA and protein synthesis, and was blocked by low temperatures. Salmonella were internalized within a membrane-bound vacuole and exhibited penetration through, but not between MDCK cells. A maximum of 14 Salmonella per MDCK cell crossed the monolayer per hour to the basolateral surface yet the monolayer remained viable and impermeable to Escherichia coli. Apical S. choleraesuis infection resulted in an increase in paracellular permeability but the MDCK intercellular contacts were not significantly disrupted. Basolateral S. choleraesuis infection was inefficient, and only small numbers of S. choleraesuis penetrated to the apical medium.

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