Evaluation of river water genotoxicity using the piscine micronucleus test

2007 ◽  
Vol 48 (6) ◽  
pp. 421-429 ◽  
Author(s):  
Serap Ergene ◽  
Tolga Çavaş ◽  
Ayla Çelik ◽  
Nurcan Köleli ◽  
Cemil Aymak
Keyword(s):  
River Water ◽  
Micronucleus Test ◽  
Piscine Micronucleus Test

scholarly journals Micronucleus test in fish for in situ evaluation of the Sinos River water quality, in Brazil

2019 ◽  
Vol 41 ◽  
pp. e38
Author(s):  
Leonardo Airton Ressel Simões ◽  
Thaís Dalzochio ◽  
Angélica Goldoni ◽  
Mateus Santos de Souza ◽  
Gabriela Zimmermann Prado Rodrigues ◽  
...  
Keyword(s):  
Water Quality ◽  
River Water ◽  
Micronucleus Test ◽  
Physicochemical Analysis ◽  
River Water Quality ◽  
Urban Wastewater ◽  
Nuclear Abnormalities ◽  
Total Phosphorous ◽  
Urban Wastewater Treatment

The Sinos River basin is impacted by industrial and agricultural activities, as well as by low rates of urban wastewater treatment. The purpose of this study was to monitor de Sinos River water quality using the micronucleus test in fish and the analysis of water physicochemical parameters. Bryconamericus iheringii specimens were captured in December 2013 (summer) and July 2014 (winter) at two sites located in the Sinos River: Caraá, in the upper section of the basin, and Parobé, in the middle section. After capture, animals were immediately killed and blood samples were collected for the micronucleus test. No significant differences were observed in micronucleus frequencies between sites and sampling periods. However, in the summer, nuclear abnormalities frequencies observed in Parobé were significantly higher than in Caraá. A higher frequency of nuclear abnormalities was also found in fish captured in Caraá during winter, in comparison with frequencies found in the summer. The results for the water physicochemical analysis showed values of total phosphorous, aluminum, lead, copper and iron above the allowed limits established by the Brazilian legislation. The nuclear abnormalities induction found in the present study may be associated to the presence of cytogenotoxic substances in the water.

The piscine micronucleus test to assess the impact of pollution on the Japaratuba river in Brazil

2006 ◽  
Vol 47 (3) ◽  
pp. 219-224 ◽  
Author(s):  
Silmara de Moraes Pantaleão ◽  
Ayda Vera Alcântara ◽  
José do Patrocínio Hora Alves ◽  
Mário Antônio Spanó
Keyword(s):  
Micronucleus Test ◽  
The Impact ◽  
Piscine Micronucleus Test

Evaluation of genotoxicity from Nilufer Stream (Bursa/Turkey) water using piscine micronucleus test

2010 ◽  
Vol 48 (8-9) ◽  
pp. 2443-2447 ◽  
Author(s):  
Senay Summak ◽  
Nilufer Cinkilic Aydemir ◽  
Ozgür Vatan ◽  
Dilek Yılmaz ◽  
Tolga Zorlu ◽  
...  
Keyword(s):  
Micronucleus Test ◽  
Piscine Micronucleus Test

Application of micronucleus in vitro assay to micropollutants in river water

1997 ◽  
Vol 35 (8) ◽  
pp. 9-13 ◽  
Author(s):  
Youn-Son Chung ◽  
Kazuhiro Ichikawa ◽  
Hideo Utsumi
Keyword(s):  
River Water ◽  
Mammalian Cells ◽  
Micronucleus Test ◽  
In Vitro Test ◽  
Vitro Assay ◽  
Mutagenicity Test ◽  
Vitro Test ◽  
Kanagawa Prefecture ◽  
The Difference

To determine the genotoxicity of river water towards mammalian cells, we applied Micronucleus in vitro test using mammalian cells to the samples taken from river Tamagawa located between Tokyo and Kanagawa prefecture. Water samples were condensed by Sep-pak cartridges and extracted by dichloromethane and methanol. Positive genotoxicity was observed in methanol extracts from sampling stations of Hinobashi and Marukobashi, while no dichloromethane extracts showed genotoxicity, suggesting that polar genotoxic micropollutants may be contained in the water of Tamagawa, at least in its down-stream. Significantly high mutagenicity also detected from Hinobashi and Marukobashi by Ames mutagenicity test using Salmonella Typhimurium, and some difference was obtained in sensitivity between the two methods. This may arise from the difference in species used, that is, mammalian cells in micronucleus test and bacteria in Ames mutagenicity test.

Assessment of the Piscine Micronucleus Test as an in situ Biological indicator of Chemical Contaminant Effects

1990 ◽  
Vol 47 (11) ◽  
pp. 2123-2136 ◽  
Author(s):  
Kenneth R. Carrasco ◽  
Karen L. Tilbury ◽  
Mark S. Myers
Keyword(s):  
Micronucleus Test ◽  
Biological Indicator ◽  
Chemical Contamination ◽  
Contaminant Exposure ◽  
White Croaker ◽  
Blood Smears ◽  
Chemical Contaminant ◽  
Piscine Micronucleus Test ◽  
Reference Areas

The piscine micronucleus test was assessed for sensitivity and practicality as an in situ biological indicator of chemical contaminant exposure by an application to the peripheral blood of wild white croaker (Genyonemus lineatus) collected from polluted and reference areas along the California coast. The several types of variations from the usual erythrocyte nuclear morphology observed in the blood smears collected for this study were described and the frequencies were compared with measured levels of certain classes of contaminants in white croaker bile and liver, and in sediment collected at each site, in addition, the frequencies were also compared with the prevalence of idiopathic lesions in the livers of the croaker. However, the piscine micronucleus test appeared to lack sensitivity to the presence and effects of the measured contaminants because no association was detected between the frequencies of the erythrocyte nuclear variations and levels of chemical contamination, even though most of the observed variations resembled the published descriptions of variations presumed to be nuclear lesions in earlier investigations. Even if the test had been found to be sensitive, the Sow and variable frequencies of the observed nuclear lesions would present a serious difficulty to the useful application of the test.

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