Assessment of the Piscine Micronucleus Test as an in situ Biological indicator of Chemical Contaminant Effects

1990 ◽  
Vol 47 (11) ◽  
pp. 2123-2136 ◽  
Author(s):  
Kenneth R. Carrasco ◽  
Karen L. Tilbury ◽  
Mark S. Myers
Keyword(s):  
Micronucleus Test ◽  
Biological Indicator ◽  
Chemical Contamination ◽  
Contaminant Exposure ◽  
White Croaker ◽  
Blood Smears ◽  
Chemical Contaminant ◽  
Piscine Micronucleus Test ◽  
Reference Areas

The piscine micronucleus test was assessed for sensitivity and practicality as an in situ biological indicator of chemical contaminant exposure by an application to the peripheral blood of wild white croaker (Genyonemus lineatus) collected from polluted and reference areas along the California coast. The several types of variations from the usual erythrocyte nuclear morphology observed in the blood smears collected for this study were described and the frequencies were compared with measured levels of certain classes of contaminants in white croaker bile and liver, and in sediment collected at each site, in addition, the frequencies were also compared with the prevalence of idiopathic lesions in the livers of the croaker. However, the piscine micronucleus test appeared to lack sensitivity to the presence and effects of the measured contaminants because no association was detected between the frequencies of the erythrocyte nuclear variations and levels of chemical contamination, even though most of the observed variations resembled the published descriptions of variations presumed to be nuclear lesions in earlier investigations. Even if the test had been found to be sensitive, the Sow and variable frequencies of the observed nuclear lesions would present a serious difficulty to the useful application of the test.

scholarly journals In situ loop-mediated isothermal amplification (LAMP) for identification of Plasmodium species in wide-range thin blood smears

2018 ◽  
Vol 17 (1) ◽  
Author(s):  
Muneaki Hashimoto ◽  
Hirokazu Sakamoto ◽  
Yusuke Ido ◽  
Masato Tanaka ◽  
Shouki Yatsushiro ◽  
...  
Keyword(s):  
Plasmodium Species ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Blood Smears ◽  
Wide Range ◽  
Thin Blood Smears

scholarly journals Cytotoxic and Genotoxic Evaluation of the Aqueous and Hydroalcoholic Leaf and Bark Extracts of Crataegus oxyacantha in Murine Model

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2217
Author(s):  
Fany Renata Aguilera-Rodríguez ◽  
Ana Lourdes Zamora-Perez ◽  
Clara Luz Galván-Moreno ◽  
Rosalinda Gutiérrez-Hernández ◽  
Claudia Araceli Reyes Estrada ◽  
...  
Keyword(s):  
Murine Model ◽  
Leaf Extract ◽  
Micronucleus Test ◽  
Genotoxic Effects ◽  
Cytotoxic Effects ◽  
Blood Smears ◽  
Polychromatic Erythrocytes ◽  
Aqueous Leaf Extract ◽  
Peripheral Blood Smears ◽  
Sampling Times

Crataegus oxyacantha has been mainly used in traditional medicine for the treatment of cardiovascular diseases. However, its safety profile has not been fully established, since only the genotoxic effects of C. oxyacantha fruit have been described. Therefore, the objective of this work was evaluating the cytotoxicity and genotoxicity of the aqueous and hydroalcoholic leaf and bark extracts of C. oxyacantha by means of the micronucleus test in a murine model. Doses of 2000, 1000, and 500 mg/kg of both extracts were administered orally for 5 days in mice of the Balb-C strain. Peripheral blood smears were performed at 0, 24, 48, 72, and 96 h after each administration. The number of polychromatic erythrocytes (PCEs), micronucleated polychromatic erythrocytes (MNPCEs), and micronucleated erythrocytes (MNEs) was determined at the different sampling times. Our results showed that the leaf and bark of C. oxyacantha increase the number of MNEs at the 2000 mg/kg dose, and only the aqueous leaf extract decreases the number of PCEs at the same dose. Therefore, the aqueous and hydroalcoholic leaf and bark extracts of C. oxyacantha showed genotoxic effects, and only the aqueous leaf extract exhibited cytotoxic effects.

Evaluation of river water genotoxicity using the piscine micronucleus test

2007 ◽  
Vol 48 (6) ◽  
pp. 421-429 ◽  
Author(s):  
Serap Ergene ◽  
Tolga Çavaş ◽  
Ayla Çelik ◽  
Nurcan Köleli ◽  
Cemil Aymak
Keyword(s):  
River Water ◽  
Micronucleus Test ◽  
Piscine Micronucleus Test

scholarly journals Detection of chimeric BCR-ABL genes on bone marrow samples and blood smears in chronic myeloid and acute lymphoblastic leukemia by in situ hybridization

Blood ◽  
1994 ◽  
Vol 83 (7) ◽  
pp. 1922-1928 ◽  
Author(s):  
M Bentz ◽  
G Cabot ◽  
M Moos ◽  
MR Speicher ◽  
A Ganser ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Acute Lymphoblastic Leukemia ◽  
Yeast Artificial Chromosome ◽  
Lymphoblastic Leukemia ◽  
Philadelphia Chromosome ◽  
High Specificity ◽  
Breakpoint Cluster Region ◽  
Cluster Region ◽  
Blood Smears

Abstract The presence of BCR-ABL fusion genes has important diagnostic and prognostic implications in chronic myeloid leukemia (CML) and acute lymphoblastic leukemia (ALL). The CML-specific chimeric BCR-ABL gene with a break involving the major breakpoint cluster region (M-bcr) of the BCR-gene has been detected by means of fluorescence in situ hybridization (FISH). In this study, we present a FISH protocol that allows the detection of breaks in both the major and the minor breakpoint cluster region (m-bcr). Three hybridization signals of D107F9, a yeast artificial chromosome (YAC)-derived probe spanning the breakpoint regions of the BCR gene, were indicative of the translocation events. To increase the specificity further, this probe was combined with cos-abl 8, a cosmid probe flanking the breakpoint within the ABL gene for dual-color hybridization. Samples of 21 patients with CML, the ALL-derived cell line SUP-B15, and of seven patients with Philadelphia chromosome (Ph1)-positive ALL (three of them with breakpoints within m-bcr) were examined. BCR-ABL fusion was detected in all cases with high specificity (false-positive nuclei: mean, 0.1%). On cytogenetic preparations, the percentages of BCR-ABL- positive interphase cells ranged from 53% to 91%. Comparable efficiencies were achieved on blood smears. In conclusion, hybridization with D107F9 and cos-abl 8 allows unambiguous diagnosis of BCR-ABL genes and is likely to become an important tool for the monitoring of therapies in patients with CML and ALL.

Comparison of in vitro and in situ genotoxicity in the Danube River by means of the comet assay and the micronucleus test

2010 ◽  
Vol 700 (1-2) ◽  
pp. 11-17 ◽  
Author(s):  
Melanie Boettcher ◽  
Stefanie Grund ◽  
Steffen Keiter ◽  
Thomas Kosmehl ◽  
Georg Reifferscheid ◽  
...  
Keyword(s):  
Comet Assay ◽  
Micronucleus Test ◽  
Danube River ◽  
The Danube River

scholarly journals Bone marrow histopathology in POEMS syndrome: a distinctive combination of plasma cell, lymphoid, and myeloid findings in 87 patients

Blood ◽  
2011 ◽  
Vol 117 (24) ◽  
pp. 6438-6444 ◽  
Author(s):  
Linda N. Dao ◽  
Curtis A. Hanson ◽  
Angela Dispenzieri ◽  
William G. Morice ◽  
Paul J. Kurtin ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Plasma Cell ◽  
Plasma Cells ◽  
Poems Syndrome ◽  
Skin Changes ◽  
Bone Marrow Histopathology ◽  
Blood Smears ◽  
Peripheral Blood Smears ◽  
Lymphoid Aggregates

Abstract POEMS is an uncommon syndromic disorder characterized by polyneuropathy, organomegaly, endocrinopathy, monoclonal protein, and skin changes. There are few descriptions of the bone marrow pathology of POEMS; therefore, peripheral blood smears and bone marrow aspirates and biopsies from 87 patients (143 total, 67 pretreatment, 76 posttreatment cases) with POEMS were studied. Plasma cell clonality was analyzed by flow cytometry, immunohistochemistry, and/or in situ hybridization. Monotypic plasma cells were detected in 44 pretreatment cases (66%); the majority of plasma cells expressed λ light chain (91%). The monotypic plasma cells typically were present in a background of increased polytypic plasma cells. Lymphoid aggregates were found in 33 (49%) pretreatment cases and in most cases were rimmed by plasma cells (97%). Megakaryocyte hyperplasia (36 cases) and clusters (62 cases) were frequent; however, none of the 43 cases tested had the JAK2V617F mutation. In summary, we have identified a novel constellation of features that should strongly suggest POEMS syndrome as part of the differential diagnosis. The constellation of λ-restricted monoclonal gammopathy, plasma cell rimming around lymphoid aggregates, and megakaryocytic hyperplasia in a bone marrow is highly suggestive of this diagnosis, especially in the context of a peripheral neuropathy.

Predicting the impact of perturbations on salmon (Oncorhynchus spp.) communities: implications for monitoring

2004 ◽  
Vol 61 (7) ◽  
pp. 1166-1175 ◽  
Author(s):  
M R Arkoosh ◽  
L Johnson ◽  
P A Rossignol ◽  
T K Collier
Keyword(s):  
Pacific Northwest ◽  
Anthropogenic Factors ◽  
Valuable Insight ◽  
Community Members ◽  
Contaminant Exposure ◽  
Community Model ◽  
The Pacific ◽  
Chemical Contaminant ◽  
The Impact ◽  
Insight Into

Twenty-six salmon (Oncorhynchus spp.) stocks from the Pacific Northwest are listed as either threatened or endangered. A number of anthropogenic factors, likely including degradation of habitat by chemical contaminant exposure, have contributed to their decline. Techniques that can assess injury or judge the efficacy of regulatory actions on the recovery of this species are needed. We strive to understand why a population is changing by examining changes in their intrinsic birth rates, death rates, and (or) growth rates. However, salmon populations are influenced by other species in the community. To address this issue, we developed a parsimonious three-trophic-level community model consisting of prey, salmon, and parasites and examined the model's response to one anthropogenic factor (contaminant exposure) using qualitative analysis. This community model may not only provide valuable insight into salmon survival but also may broaden the approaches available to elucidate direct and indirect effects. We demonstrate analytically that some community members, possibly salmon themselves, might be ambiguous or unreliable variables to monitor. We also demonstrate that other species in the community, such as parasites, may be more sensitive than salmon in monitoring the influence of anthropogenic factors such as contaminants.

scholarly journals Biomonitoring findings for occupational lead exposure in battery and ceramic tile workers using biochemical markers, alkaline comet assay, and micronucleus test coupled with fluorescence in situ hybridisation

2020 ◽  
Vol 71 (4) ◽  
pp. 339-352
Author(s):  
Vilena Kašuba ◽  
Mirta Milić ◽  
Davor Želježić ◽  
Marin Mladinić ◽  
Alica Pizent ◽  
...  
Keyword(s):  
Comet Assay ◽  
Aminolevulinic Acid ◽  
Micronucleus Test ◽  
In Situ Hybridisation ◽  
Serum Folate ◽  
Fluorescence In Situ Hybridisation ◽  
Alkaline Comet Assay ◽  
Alad Activity ◽  
Exposed Workers

AbstractManufacture of lead-containing products has long been associated with various health risks. To get an insight into the related genotoxic risks, we conducted a biomonitoring study in 50 exposed workers and 48 matched controls using a battery of endpoints that sensitively detect the extent of genome instability in peripheral blood lymphocytes. The levels of primary DNA damage were estimated with the alkaline comet assay, while cytogenetic abnormalities were determined with the cytokinesis-block micronucleus (CBMN) cytome assay. Additionally, CBMN slides of 20 exposed and 16 control participants were subjected to fluorescence in situ hybridisation (FISH), coupled with pancentromeric probes to establish the incidence of centromere-positive micronuclei, nuclear buds, and nucleoplasmic bridges. Blood lead levels (B-Pb) were measured with atomic absorption spectrometry. To further characterise cumulative effects of occupational exposure, we measured erythrocyte protoporphyrin (EP) concentrations and delta-aminolevulinic acid dehydratase (ALAD) activity in blood. We also assessed the influence of serum folate (S-folate) and vitamin B12 (S-B12) on genome stability. Compared to controls, occupationally exposed workers demonstrated significantly higher B-Pb (298.36±162.07 vs 41.58±23.02), MN frequency (18.71±11.06 vs 8.98±7.50), centromere positive MN (C+ MN) (8.15±1.8 vs 3.69±0.47), and centromere negative MN (C- MN) (14.55±1.80 vs 4.56±0.89). Exposed women had significantly higher comet tail intensity (TI) and length (TL) than control women. Furthermore, workers showed a positive correlation between age and nuclear buds and MN, between MN and years of exposure, and between S-B12 levels and TI and ALAD activity, while a negative correlation was found between TI and B-Pb. These findings suggest that occupational settings in the manufacture of lead-containing products pose significant genotoxic risks, which calls for developing more effective work safety programmes, including periodical monitoring of B-Pb and genetic endpoints.

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