The piscine micronucleus test to assess the impact of pollution on the Japaratuba river in Brazil

2006 ◽  
Vol 47 (3) ◽  
pp. 219-224 ◽  
Author(s):  
Silmara de Moraes Pantaleão ◽  
Ayda Vera Alcântara ◽  
José do Patrocínio Hora Alves ◽  
Mário Antônio Spanó
Keyword(s):  
Micronucleus Test ◽  
The Impact ◽  
Piscine Micronucleus Test

Evaluation of river water genotoxicity using the piscine micronucleus test

2007 ◽  
Vol 48 (6) ◽  
pp. 421-429 ◽  
Author(s):  
Serap Ergene ◽  
Tolga Çavaş ◽  
Ayla Çelik ◽  
Nurcan Köleli ◽  
Cemil Aymak
Keyword(s):  
River Water ◽  
Micronucleus Test ◽  
Piscine Micronucleus Test

scholarly journals ANALYSIS OF THE INTERRELATION OF CYTOGENETIC HOMEOSTASIS AND OXIDATIVE STRESS IN THE ORGANISM OF GOBY FISH (GOBIIDAE) OF THE NORTHERN CASPIAN

2018 ◽  
Vol 13 (2) ◽  
pp. 64-72
Author(s):  
Tatiana V. Kuzina ◽  
Maya L. Galaktionova
Keyword(s):  
Lipid Peroxidation ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Micronucleus Test ◽  
Functional Dependence ◽  
Thiobarbituric Acid ◽  
The Body ◽  
Muscle Tissues ◽  
Fish Blood ◽  
The Impact

Aim. The aim is to investigate the relationship between the content of micronuclei, destructive disorders in the nuclei of erythrocytes of peripheral blood of the goby fish and intensity of lipid peroxidation in fish tissues. Methods. Was used the method of quantitative accounting of morphologically altered red blood cells of peripheral blood of goby fish. Evaluation of cytogenetic disorders in fish blood cells was carried out based on a micronucleus test. Samples of muscle tissues and liver were sampled for biochemical analyzes. The rates of lipid peroxidation (LPO level) were determined by a method based on the reaction of malondialdehyde and thiobarbituric acid, the end product of lipid peroxidation. Results. A correlation was found between the rates of spontaneous LPO, ascorbate-dependant LPO and the accumulation of malondialdehyde in the liver of fish and changes in the nucleus of erythrocytes (R2 =0,8; R2 =0,6; R2 =0,7, respectively). Conclusions. We established the functional dependence which justifies the need to use cytogenetic markers to assess the impact of adverse environmental factors on the body of hydrobionts.

Evaluation of genotoxicity from Nilufer Stream (Bursa/Turkey) water using piscine micronucleus test

2010 ◽  
Vol 48 (8-9) ◽  
pp. 2443-2447 ◽  
Author(s):  
Senay Summak ◽  
Nilufer Cinkilic Aydemir ◽  
Ozgür Vatan ◽  
Dilek Yılmaz ◽  
Tolga Zorlu ◽  
...  
Keyword(s):  
Micronucleus Test ◽  
Piscine Micronucleus Test

EVALUACIÓN DE LA CITOGENOTOXICIDAD DE UNA CERVEZA ELABORADA INDUSTRIALMENTE Y UNA CERVEZA DE ELABORACIÓN ARTESANAL

2020 ◽  
Vol 9 (33-36) ◽  
Author(s):  
Arturo Rosas-Cipriano ◽  
Saúl Flores-Maya ◽  
Héctor Barrera-Escorcia ◽  
Sandra Gómez-Arroyo ◽  
Norberto Alarcón-Herrera ◽  
...  
Keyword(s):  
Vicia Faba ◽  
Micronucleus Test ◽  
Negative Control ◽  
Alcoholic Beverages ◽  
Chemical Components ◽  
Meristematic Cells ◽  
Craft Beer ◽  
Dunnett's Test ◽  
The Impact

<p class="Default">Beer is a complex beverage, due to the extended variety of dissolved constituents which interact with each other and may change rapidly from the moment of exposure to the environment. Much has been said about the impact on human health on the consumption of beer; excessive intake can lead to health problems, on the contrary with the moderate intake can significantly reduce DNA damage. The importance of short-term tests for carcinogens and mutagens (micronucleus test) is feasible to be applied in risk analysis and quality control of commercial alcohol beverages. The objective of this study was to evaluate the genotoxic effects in vivo of two different types of beers using <em>Vicia faba</em>micronucleus test. Faba beans were used (<em>Vicia faba var. minor</em>) that were exposed for 24, 48, 72 and 120 hours to the chemical composition of a mass-produced beer and craft beer. The cytogenotoxic effect from the beers was evaluated in root meristematic cells. Cycloheximide (5 <em>u</em>g/ml), 3.6% ethanol (v/v) and 7.5% (v/v) were used as positive controls. Data were applied by analysis of variance with individual comparisons (Dunnett’s test) with a significance criterion of <em>p</em>&lt; 0.05. The results allowed toestablishing that treatments for the mitotic index and the frequency of micronuclei in meristematic cells had significant differences compared to the negative control. In conclusion, the chemical components of these alcoholic beverages and acetaldehyde derived from metabolized ethanol by the root neristematic cells of <em>V.faba</em>did not induce high frequencies of micronuclei, in other words, they were slightly genotoxic and mildly toxic by decreasing or increasing of cell division on root meristem cells of <em>V. faba</em>.</p>

scholarly journals Assessment of periodontal conditions in workers exposed to harmful substances using the micronucleus test

2020 ◽  
Vol 99 (9) ◽  
pp. 956-960
Author(s):  
Iskander I. Zaydullin ◽  
Lilija K. Karimova ◽  
Milyausha F. Kabirova ◽  
Guzjal’ R. Sadrtdinova ◽  
Rasima R. Galimova ◽  
...  
Keyword(s):  
Negative Impact ◽  
Inflammatory Diseases ◽  
Micronucleus Test ◽  
Periodontal Diseases ◽  
Processing Plant ◽  
Periodontal Tissues ◽  
Buccal Epithelial Cells ◽  
Significant Difference ◽  
Harmful Substances ◽  
The Impact

The purpose of this study was to identify the possibility of using a micronucleus test to assess the impact of harmful chemicals on the condition of periodontal tissues of employees of a mining and processing plant. Material and methods. Dental examination was conducted for 40 employees of the mining and processing plant, exposed to harmful substances with an experience of more than 10 years, 36 employees of the plant with a contact time of not more than 10 years, and 46 people with a healthy periodontium. The analysis of buccal epithelial cells for the presence of chromosomal aberrations using the micronucleus test. Results. The prevalence of inflammatory diseases of periodontal tissues in the groups of workers of the plant was 100%. A statistically significant difference was found in the frequency of cells with micronuclei and protrusions of the nucleus in the group of internship workers compared with other groups (p = 0.001). The frequency of cells with two nuclei in the workers of the plant in the groups was at the same level and significantly exceeded the value of the index for a group of people with a healthy periodontium (p = 0.002 and p = 0.006). The frequency of occurrence of cells with vacuolization of the nucleus in a group of workers with an experience of more than 10 years more than doubles that of a group of individuals with a healthy periodontium (p = 0.009). Conclusion. Occupational factors have a significant impact on the development and course of inflammatory periodontal diseases in mining and processing complex employees. Studies using the micronuclear test indicate a negative impact of a complex of harmful substances on the development and course of inflammatory periodontal diseases.

Assessment of the Piscine Micronucleus Test as an in situ Biological indicator of Chemical Contaminant Effects

1990 ◽  
Vol 47 (11) ◽  
pp. 2123-2136 ◽  
Author(s):  
Kenneth R. Carrasco ◽  
Karen L. Tilbury ◽  
Mark S. Myers
Keyword(s):  
Micronucleus Test ◽  
Biological Indicator ◽  
Chemical Contamination ◽  
Contaminant Exposure ◽  
White Croaker ◽  
Blood Smears ◽  
Chemical Contaminant ◽  
Piscine Micronucleus Test ◽  
Reference Areas

The piscine micronucleus test was assessed for sensitivity and practicality as an in situ biological indicator of chemical contaminant exposure by an application to the peripheral blood of wild white croaker (Genyonemus lineatus) collected from polluted and reference areas along the California coast. The several types of variations from the usual erythrocyte nuclear morphology observed in the blood smears collected for this study were described and the frequencies were compared with measured levels of certain classes of contaminants in white croaker bile and liver, and in sediment collected at each site, in addition, the frequencies were also compared with the prevalence of idiopathic lesions in the livers of the croaker. However, the piscine micronucleus test appeared to lack sensitivity to the presence and effects of the measured contaminants because no association was detected between the frequencies of the erythrocyte nuclear variations and levels of chemical contamination, even though most of the observed variations resembled the published descriptions of variations presumed to be nuclear lesions in earlier investigations. Even if the test had been found to be sensitive, the Sow and variable frequencies of the observed nuclear lesions would present a serious difficulty to the useful application of the test.

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