scholarly journals Piscine micronucleus test and the comet assay reveal genotoxic effects of Atrazine herbicide in the neotropical fish Rhamdia quelen

2015 ◽  
Vol 10 (1) ◽  
pp. 55-60 ◽  
Author(s):  
L.D.S. Piancini ◽  
G.S. Santos ◽  
F.H. Tincani ◽  
M.M Cestari
Keyword(s):  
Comet Assay ◽  
Micronucleus Test ◽  
Genotoxic Effects ◽  
Neotropical Fish ◽  
Rhamdia Quelen ◽  
Piscine Micronucleus Test

Nicotine derived genotoxic effects in human primary parotid gland cells as assessed in vitro by comet assay, cytokinesis-block micronucleus test and chromosome aberrations test

2014 ◽  
Vol 28 (5) ◽  
pp. 838-846 ◽  
Author(s):  
Christian Ginzkey ◽  
Gudrun Steussloff ◽  
Christian Koehler ◽  
Marc Burghartz ◽  
Agmal Scherzed ◽  
...  
Keyword(s):  
Comet Assay ◽  
Parotid Gland ◽  
Chromosome Aberrations ◽  
Micronucleus Test ◽  
Genotoxic Effects ◽  
Gland Cells

scholarly journals Toxicity of naphthalene in the Neotropical Fish Astyanax Lacustris (Characiformes: Characidae) and Geophagus Brasiliensis (Perciformes: Cichlidae)

2017 ◽  
Vol 17 (1) ◽  
pp. 7-22 ◽  
Author(s):  
Geonildo Rodrigo Disner ◽  
Sabrina Louise Moraes Calado ◽  
Helena Cristina Silva Assis ◽  
Marta Margarete Cestari
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Micronucleus Test ◽  
Glutathione S Transferase ◽  
Neotropical Fish ◽  
Compensatory Response ◽  
Low Concentrations ◽  
Geophagus Brasiliensis ◽  
Polycyclic Aromatic ◽  
Gst Activity

Polycyclic aromatic hydrocarbons are one of the most important organic pollutants in environmental studies. The aim of this study was to assess the naphthalene acute toxicity in two fish species, Astyanax lacustris (LLcust, 1875) and Geophagus brasiliensis (Quoy & Gaimard, 1824). The fish were exposed to naphthalene (0.005, 0.03, 0.3, and 3 mgL-1) in water and after that the piscine micronucleus test in erythrocytes, comet assay in blood, liver and gill cells, glutathione S–transferase (GST) activity in the liver, and accumulation of naphthalene in the bile were performed. The susceptibility of the two species was similar and naphthalene was not genotoxic in all tested tissues. The liver GST activity may have been responsible for less damage observed in the liver while the highest DNA damage occurred in blood cells. However, low concentrations of naphthalene in water can stimulate apparent benefits, such as less DNA damage, which would be a compensatory response to an imbalance of homeostasis. The naphthalene is absorbed and can accumulate in the gall bladder, a greater accumulation of PAH was observed in A. lacustris, while G. brasiliensis did not differ from the control. The naphthalene concentrations are not genotoxic to the tested species, although they can potentially accumulate into the body.Keywords: Comet assay. Ecotoxicology. Fish. Genotoxicity. Hormesis.

scholarly journals Evaluation of the genotoxicity in fish erythrocytes to diagnose the water quality of two Amazonian estuaries using the micronucleus test and comet assay

2021 ◽  
Author(s):  
CLAUDIA ANTONIA CAMPOS RODRIGUES DE OLIVEIRA ◽  
Paulo Sergio dos Santos Souto ◽  
Dulcideia da Conceição Palheta ◽  
Marcelo de Oliveira Bahia ◽  
Lorena Araújo da Cunha ◽  
...  
Keyword(s):  
Comet Assay ◽  
Micronucleus Test ◽  
Genetic Material ◽  
Aquatic Organisms ◽  
Brazilian Amazon ◽  
Genotoxic Effects ◽  
Blood Samples ◽  
Genomic Damage ◽  
And Control

Abstract Genotoxicity studies in coastal ecosystems have been a priority in Environmental Risk Assessment (ERA). This research aimed to study the genotoxicity by the micronucleus test and comet assay in two Brazilian Amazon estuaries (anthropized and control) using Plagioscion squamosissimus as a bioindicator. Blood samples were collected from 54 specimens. No significant genotoxic effects were detected in the cells analyzed, although the highest occurrence was observed in anthropized site. The percentage of genomic damage differed between the sites studied, being always higher in anthropizes site as well. Of the nucleoids analyzed in this site, on average 28 ± 14.42% of the cells were classified in the highest damage class (4). The fish analyzed in the present study are direct influence of xenobiont agents capable of producing damage to the genetic material of aquatic organisms in both sites and, consequently, may bring consequences still little reported in studies of morphophysiological alterations in humans.

Genotoxic effects of the fungicide thiophanate-methyl on Podarcis sicula assessed by micronucleus test, comet assay and chromosome analysis

Ecotoxicology ◽  
2011 ◽  
Vol 20 (4) ◽  
pp. 885-891 ◽  
Author(s):  
T. Capriglione ◽  
S. De Iorio ◽  
F. Gay ◽  
A. Capaldo ◽  
M. C. Vaccaro ◽  
...  
Keyword(s):  
Comet Assay ◽  
Micronucleus Test ◽  
Chromosome Analysis ◽  
Genotoxic Effects ◽  
Podarcis Sicula ◽  
Thiophanate Methyl

scholarly journals Dental curing light: non-ionizing electromagnetic radiation and its possible genotoxic effects

2020 ◽  
Vol 9 (10) ◽  
pp. e4609108884
Author(s):  
Michele de Sampaio Sousa ◽  
Laryssa Roque da Silva ◽  
Danniel Cabral Leão Ferreira ◽  
Fabrício Pires Moura do Amaral ◽  
Rosemarie Brandim Marques ◽  
...  
Keyword(s):  
Comet Assay ◽  
Rattus Norvegicus ◽  
Micronucleus Test ◽  
Clinical Care ◽  
Positive Control ◽  
Distilled Water ◽  
Test Results ◽  
Control Groups ◽  
Genotoxic Effects ◽  
Curing Light

Objective: In this scientific study, we aimed to evaluate genotoxic effects in rats (Rattus norvegicus), related to different periods of exposures to the LED curing light. Methodology: For the genotoxicity evaluation, the rats received lights from the LED photopolymerizer for 40 sec, 10 min and 7-and-a-half minutes, while the negative and positive control groups were treated with distilled water and cyclophosphamide by intraperitoneal, respectively. A sample of peripheral blood was collected from the animals for the comet assay. The bone marrow was collected from each rat for the micronucleus test. Results: It was observed that in the comet assay and micronucleus test, the animals exposed to LED for 10 min, showed genotoxic damage, and they have not presented toxicity degree in the periods of 40 sec and 7-and-a-half minutes. Conclusion: It is possible to conclude that, there was genotoxic effects on the animals' teeth when exposed to the LED curing light in 10 min. However, in the periods of 40 sec, and 7- and-a-half minutes, have been not observed genotoxic effects. This means these times are safe for professional dentists in clinical care.

scholarly journals Evaluation of the Genotoxicity in Fish Erythrocytes to Diagnose the Water Quality of Two Amazonian Estuaries Using the Micronucleus Test and Comet Assay

2021 ◽  
Author(s):  
CLAUDIA ANTONIA CAMPOS RODRIGUES DE OLIVEIRA ◽  
Paulo Sergio dos Santos Souto ◽  
Dulcideia da Conceição Palheta ◽  
Marcelo de Oliveira Bahia ◽  
Lorena Araújo da Cunha ◽  
...  
Keyword(s):  
Comet Assay ◽  
Micronucleus Test ◽  
Genetic Material ◽  
Aquatic Organisms ◽  
Brazilian Amazon ◽  
Genotoxic Effects ◽  
Blood Samples ◽  
Genomic Damage ◽  
And Control

Abstract Genotoxicity studies in coastal ecosystems have been a priority in Environmental Risk Assessment (ERA). This research aimed to study the genotoxicity by the micronucleus test and comet assay in two Brazilian Amazon estuaries (anthropized and control) using Plagioscion squamosissimus as a bioindicator. Blood samples were collected from 54 specimens. No significant genotoxic effects were detected in the cells analyzed, although the highest occurrence was observed in anthropized site. The percentage of genomic damage differed between the sites studied, being always higher in anthropizes site as well. Of the nucleoids analyzed in this site, on average 28±14.42% of the cells were classified in the highest damage class (4). The fish analyzed in the present study are direct influence of xenobiont agents capable of producing damage to the genetic material of aquatic organisms in both sites and, consequently, may bring consequences still little reported in studies of morphophysiological alterations in humans.

Genotoxic effects of nanosized and fine TiO2

2009 ◽  
Vol 28 (6-7) ◽  
pp. 339-352 ◽  
Author(s):  
GCM Falck ◽  
HK Lindberg ◽  
S. Suhonen ◽  
M. Vippola ◽  
E. Vanhala ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Micronucleus Test ◽  
Genotoxic Effects ◽  
Bronchial Epithelial ◽  
Dose Dependent ◽  
Dose Dependent Effect ◽  
Fine Tio2 ◽  
In Vitro Genotoxicity

The in-vitro genotoxicity of nanosized TiO2 rutile and anatase was assessed in comparison with fine TiO2 rutile in human bronchial epithelial BEAS 2B cells using the single-cell gel electrophoresis (comet) assay and the cytokinesis-block micronucleus test. BEAS 2B cells were exposed to eight doses (1—100 μg/cm2) of titanium(IV) oxide nanosized rutile (>95%, <5% amorphous SiO2 coating; 10 × 40 nm), nanosized anatase (99.7%; <25 nm), or fine rutile (99.9%; <5 μm) for 24, 48, and 72 h. Fine rutile reduced cell viability at lower doses than nanosized anatase, which was more cytotoxic than nanosized rutile. In the comet assay, nanosized anatase and fine rutile induced DNA damage at several doses with all treatment times. Dose-dependent effects were seen after the 48- and 72-h treatments with nanosized anatase and after the 24-, 48- (in one out of two experiments), and 72-h treatments (one experiment) with fine rutile. The lowest doses inducing DNA damage were 1 μg/cm2 for fine rutile and 10 μg/cm 2 for nanosized anatase. Nanosized rutile showed a significant induction in DNA damage only at 80 μg/cm2 in the 24-h treatment and at 80 and 100 μg/ cm2 in the 72-h treatment (with a dose-dependent effect). Only nanosized anatase could elevate the frequency of micronucleated BEAS 2B cells, producing a significant increase at 10 and 60 μg/cm 2 after the 72-h treatment (no dose-dependency). At increasing doses of all the particles, MN analysis became difficult due to the presence of TiO2 on the microscopic slides. In conclusion, our studies in human bronchial epithelial BEAS 2B cells showed that uncoated nanosized anatase TiO2 and fine rutile TiO2 are more efficient than SiO 2-coated nanosized rutile TiO2 in inducing DNA damage, whereas only nanosized anatase is able to slightly induce micronuclei.

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