Taste detection threshold of human (Homo sapiens) subjects and taste preference threshold of black-handed spider monkeys (Ateles geoffroyi) for the sugar substitute isomalt

The artificial sweetener isomalt is widely used due to its low caloric, non-diabetogenic and non-cariogenic properties. Although the sweetening potency of isomalt has been reported to be lower than that of sucrose, no data on the sensitivity of humans for this polyol are available. Using an up-down, two-alternative forced choice staircase procedure we therefore determined taste detection thresholds for isomalt in human subjects (n = 10; five females and five males) and compared them to taste preference thresholds, determined using a two-bottle preference test of short duration, in a highly frugivorous nonhuman primate, the spider monkey (n = 4; one female, three males). We found that both species detected concentrations of isomalt as low as 20 mM. Both humans and spider monkeys are less sensitive to isomalt than to sucrose, which is consistent with the notion of the former being a low-potency sweetener. The spider monkeys clearly preferred all suprathreshold concentrations tested over water, suggesting that, similar to humans, they perceive isomalt as having a purely sweet taste that is indistinguishable from that of sucrose. As isomalt, like most sweet-tasting polyols, may elicit gastric distress when consumed in large quantities, the present findings may contribute to the choice of appropriate amounts and concentrations of this sweetener when it is employed as a sugar substitute or food additive for human consumption. Similarly, the taste preference threshold values of spider monkeys for isomalt reported here may be useful for determining how much of it should be used when it is employed as a low-caloric sweetener for frugivorous primates kept on a vegetable-based diet, or when medication needs to be administered orally.

Parametric Characterization of a Taste Phenotype in Rats Selectively Bred for High Versus Low Saccharin Intake

Taste signals food quality and reflects energy status and associated processes. Occidental high- and low-saccharin consuming rats (HiS, LoS) have been selectively bred for nearly 60 generations on intake of 0.1% saccharin in a 23-h two-bottle test, as a tool for studying individual differences in taste and its correlates in the domains of feeding, defensive, and social behavior. The saccharin phenotype itself has not been well characterized until now. The present series of parametric studies examined suprathreshold saccharin concentration-intake functions (Experiment 1), saccharin preference threshold (Experiments 2A and 2B), and intra- and inter-sweetener carryforward effects (Experiments 2B, 3A–3D). Results indicate high stability in line differences in behavior toward saccharin and also line-specific mutability of intake of saccharin and certain other sweeteners. Methodological and conceptual implications are discussed.

Preference for linoleic acid in obesity-prone and obesity-resistant rats is attenuated by the reduction of CD36 on the tongue

Differential sensing of dietary fat and fatty acids by the oral cavity is proposed to regulate the susceptibility to obesity. In the current experiments, animals that differ in their susceptibility to obesity were used to investigate the influence of the oral cavity on the preference for the polyunsaturated fatty acid, linoleic acid. In experiment 1, the preference for differing concentrations of linoleic acid was determined in obesity-prone Osborne-Mendel (OM) and obesity-resistant S5B/Pl (S5B) rats. The preference threshold for linoleic acid was lower in S5B rats, compared with OM rats. To determine whether differences in linoleic acid preference threshold were related to innate strain differences in the fatty acid receptors on the tongue, the expression of GPR120, GPR40, and CD36 on the circumvallate papillae were assessed in OM and S5B rats. Results indicated that the expression of CD36, GPR40, and GPR120 did not differ between these two strains. Numerous studies have examined the role of CD36 on fat intake; therefore, in experiment 3, RNA interference was used to decrease the expression of CD36 on the tongues of OM and S5B rats, and the effect of decreased CD36 expression on linoleic acid preference was determined. CD36 siRNA attenuated linoleic acid preference for the most preferred concentration in both OM and S5B rats. Overall, these data indicate that there are innate differences in the preference threshold for linoleic acid in obesity-prone and obesity-resistant rats. Experimentally reducing the expression of CD36 on the circumvallate papillae attenuated the preference for linoleic acid in both strains.

Room Temperature Recovery in Rolled Polycrystalline Copper after many Years

Eight years ago recrystallization of OFE (oxygen-free electronic) copper was examined in detail using various techniques. In 2008 exactly the same material was measured using EBSD microscopy. The deformed state and fully recrystallized state have been analyzed and compared with data obtained eight years ago. The stored energy (SE) estimated by Image Quality (IQ) analysis was compared in these two cases. A significant amount of recovery took place in the sample, but only in some texture components. Some others present more or less the same SE as eight years ago. The textures of recrystallized samples were compared. We observed that the difference in SE distribution between the two deformed state has an influence on the final textures after recrystallization. Our study confirms the hypothesis that if a grain (orientation) has distinctly lower SE than other orientations - it has the highest growth preference (threshold hypothesis). Such grains (orientations) are dominant in the recrystallization texture.

Taste responses of calves to various concentrations of different salts

SUMMARYExperiments were conducted to observe the taste responses of male and female calves to various levels of sodium salts, chloride solutions, and trace minerals. Animals were penned individually and given a completely pelleted ration ad libitum. Trace mineralized salt was freely available.Using water as a basis for determination, a non-discrimination zone was established. This zone represented an intake ranging from 63 to 37%. Preference threshold was set at 80% of intake, while the rejection threshold was set at 20% of intake. Responses were determined by expressing the intake of the test solution as a percentage of the total intake during the test period. Responses at a given chemical concentration were measured during two 8-hr periods.Taste response observations were made on the following: 0·02, 0·08, 0·32 or 1·25% sodium chloride (NaCl), sodium sulphate (Na2SO4), sodium bicarbonate (NaHCO3), sodium carbonate, monobasic sodium phosphate (NaH2PO4), dibasic sodium phosphate (Na2HPO4), ammonium chloride, potassium chloride, calcium chloride, magnesium chloride, and manganese chloride. In addition, observations were made when 0·02, 0·08, 0·32 or 1·25% NaHCO3, Na2SO4, or Na2HPO4 were offered in opposition to equal concentrations of NaCl. The responses to 5, 10, 20, 40 and 60 p.p.m. concentrations of cupric chloride, zinc chloride, molybdenum trioxide and cobaltous chloride were observed also.