Effect of siRNA Interference with Upregulated Gene 11 Expression on the Biological Functions of Osteosarcoma Cell Line MG63 and Their Mechanisms

This study investigated the effect of siRNA interference with URG11 expression on biological function of osteosarcoma cell line MG63 and its mechanism. MG63 cells cultured in vitro were apportioned to make up a control group (not transfected), NC-siRNA group (transfected nonspecific NC-siRNA), and URG11-siRNA group (transfected URG11-siRNA). The expression of URG11 was detected through reverse-transcription polymerase chain reaction (RT-PCR). The metastasis, infiltration, and apoptosis of MG63 cells were examined through CCK-8, Transwell, and flow cytometry techniques, respectively. The expressions of URG11 protein in each group of cells and of proteins β-catenin, c-Myc, cyclin D1, MMP-2, and survivin, which are related to the Wnt/β-catenin signal pathway, were visualized by the Western blot method. In comparison with the control group, transfected URG11-siRNA may reduce the expression levels of URG11 and URG11 mRNA, β-catenin, CyclinD1, c-Myc, MMP-2, and survivin proteins in MG63 cells, and may decrease the metastasis and infiltration of MG63 cells and enhance apoptosis; nevertheless, there was no obvious change in MG63 cells after NC-siRNA transfection. The proliferation and infiltration of MG63 cells can be inhibited by interference with URG11 expression, and the apoptosis of MG63 cells can be promoted by interference with URG11 expression. The mechanism of interference with URG11 expression may relate to inhibiting the activation of the Wnt/β-catenin pathway.

Incorporation of Human Osteoblast Cells and Osteoblast-Like Cells into Porous Hydroxyapatite Scaffolds

The object of this study was to investigate methods of seeding cells onto porous hydroxyapatite granules with the aim of optimising cell attachment. Two cells types were used; an osteosarcoma cell line, MG63, and human osteoblasts (HOBs) isolated from trabecular bone. Several conditions were investigated to determine their effect on cell attachment. These included varying the initial seeding concentration, pre-adsorption of the granules with the adhesion protein fibronectin and the use of mechanical agitation. Human osteoblasts and MG63 osteosarcoma cells attached to both dense and porous HA granules but with a low seeding efficiency while seeding was not significantly improved by pre-coating scaffolds with fibronectin or by introducing fluid flow.