Inhibitory effect of MSH6 gene silencing in combination with cisplatin on cell proliferation of human osteosarcoma cell line MG63

2018 ◽  
Vol 234 (6) ◽  
pp. 9358-9369 ◽  
Author(s):  
Hu‐Cheng Liu ◽  
Jin Zeng ◽  
Bin Zhang ◽  
Xu‐Qiang Liu ◽  
Min Dai
Keyword(s):  
Cell Proliferation ◽  
Gene Silencing ◽  
Cell Line ◽  
Inhibitory Effect ◽  
Osteosarcoma Cell ◽  
Human Osteosarcoma Cell Line ◽  
Human Osteosarcoma ◽  
Msh6 Gene ◽  
Human Osteosarcoma Cell ◽  
Osteosarcoma Cell Line Mg63

scholarly journals Ladder-like amplification of the type I interferon gene cluster in the human osteosarcoma cell line MG63

2008 ◽  
Vol 16 (8) ◽  
pp. 1177-1192 ◽  
Author(s):  
Narasimharao V. Marella ◽  
Michael J. Zeitz ◽  
Kishore S. Malyavantham ◽  
Artem Pliss ◽  
Sei-ichi Matsui ◽  
...  
Keyword(s):  
Cell Line ◽  
Gene Cluster ◽  
Type I Interferon ◽  
Type I ◽  
Osteosarcoma Cell ◽  
Human Osteosarcoma Cell Line ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cell ◽  
Interferon Gene ◽  
Osteosarcoma Cell Line Mg63

scholarly journals Effect of acetaminophen (paracetamol) on human osteosarcoma cell line MG63

2010 ◽  
Vol 31 (11) ◽  
pp. 1495-1499 ◽  
Author(s):  
Lourdes Díaz-Rodríguez ◽  
Olga García-Martínez ◽  
Manuel Arroyo-Morales ◽  
Belén Rubio-Ruiz ◽  
Concepción Ruiz
Keyword(s):  
Cell Line ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
Human Osteosarcoma Cell Line ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cell ◽  
Osteosarcoma Cell Line Mg63

scholarly journals Artesunate Decreases β-Catenin Expression, Cell Proliferation and Apoptosis Resistance in the MG-63 Human Osteosarcoma Cell Line

2017 ◽  
Vol 43 (5) ◽  
pp. 1939-1949 ◽  
Author(s):  
Peng Chen ◽  
Wan-Li Gu ◽  
Ming-Zhi Gong ◽  
Jun Wang ◽  
Dong-Qing Li
Keyword(s):  
Cell Proliferation ◽  
Cell Line ◽  
Western Blotting ◽  
Mtt Assay ◽  
Control Group ◽  
Osteosarcoma Cell ◽  
Human Osteosarcoma Cell Line ◽  
Expression Levels ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cell

Background: This study aims to determine the effects of artesunate on proliferation, apoptosis and β-catenin expression in the human osteosarcoma cell line MG-63. Methods: MG-63 cells in the logarithmic growth phase were collected and cultured with different concentrations of artesunate (12.5 µg/mL, 25 µg/mL and 50 µg/mL) for 24 h, 48 h and 72 h. The total number of MG-63 cells and the morphological changes were observed under an inverted microscope. The MTT assay was adopted to test the inhibition rate (IR) of cell growth. The apoptosis rate was detected using annexin V/propidium iodide (PI) staining. Cell cycle distribution was identified by flow cytometry (FCM), and the expression levels of β-catenin, cyclins and cyclin dependent kinases (CDKs) were measured using Western blotting. Results: The results of the MTT assay indicated that artesunate could remarkably inhibit MG-63 cell proliferation compared with the rates in the untreated control group (0 µg/mL artesunate), and the inhibitory effect was dose-dependent. The apoptosis rate of MG-63 cells was elevated as the concentration of artesunate increased, and all the rates were significantly higher than that in the control group. Additionally, as the artesunate concentration increased, the proportion of MG-63 cells in G0/G1 phase gradually declined whereas that of cells in the G2/M and S phases increased. Western blotting confirmed that a higher concentration of artesunate reduced the expression levels of β-catenin, cyclin A, cyclin D1 and CDK1 and increased the expression levels of cyclin B1; however, artesunate had no impact on CDK2 expression in MG-63 cells. Conclusion: These results demonstrated that artesunate can inhibit β-catenin expression and cell proliferation as well as promote cell apoptosis in MG-63 cells, which indicates that artesunate may serve as a promising drug in the clinical treatment of osteosarcoma.

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