Capability of Immobilized Clostridium beijerinckii TISTR 1461 on Lotus Stalk Pieces to Produce Butanol from Sugarcane Molasses

Immobilized Clostridium beijerinckii TISTR 1461 was used to enhance the butanol production efficiency from sugarcane molasses. Lotus stalk (LS) pieces were used as carriers for cell immobilization. Sugarcane molasses containing 50 g/L of sugar supplemented with 1 g/L of yeast extract was found to be an appropriate medium for bacterial cell immobilization on the LS pieces. Carrier size (4, 12 and 20 mm in length) and carrier loading (1:15, 1:30 and 1:45, w/v) were optimized for high levels of butanol production using response surface methodology (RSM). The batch fermentation was carried out under anaerobic conditions in 1 L screw-capped bottles at 37 °C and an agitation rate of 150 rpm. It was found that the optimum conditions for the butanol production were the carrier size of 4 mm and carrier loading of 1:31 (w/v). Under these conditions, the butanol concentration (PB) was 12.89 g/L, corresponding to the butanol productivity (QB) of 0.36 g/L∙h and butanol yield (YB/S) of 0.36 g/g. These values were higher than those using free cells (PB, 10.20 g/L, QB, 0.28 g/L∙h and YB/S, 0.32 g/g). In addition, it was found that a 24 h incubation time for cell immobilization was appropriate for the immobilization process, which was confirmed by the results of the scanning electron microscope (SEM) and atomic force microscopy (AFM) images and specific surface area measurement. When the fermentation using the immobilized cells was carried out in a stirred-tank reactor (STR), column reactor (CR) and CR coupled with STR, the results showed that all reactors could be used to produce butanol production from the immobilized cells on LS pieces. However, the PB using CR and CR coupled with STR were only 75% and 45% of those using the screw-capped bottle and STR.

Catalyst Performance Studies on the Guerbet Reaction in a Continuous Flow Reactor Using Mono- and Bi-Metallic Cu-Ni Porous Metal Oxides

Higher alcohols like 1-butanol are considered important biofuels with superior properties compared to the more readily available bio-ethanol. An attractive route to prepare 1-butanol from ethanol is the Guerbet reaction. We here report the use of hydrotalcite-derived mono- (Cu-PMO or Ni-PMO) and bi-metallic (CuNi-PMO) porous metal oxide catalysts for the Guerbet coupling of ethanol to 1-butanol in a continuous flow reactor (320 °C, 0.1 MPa, LHSV = 15 mL g−1 h−1) at extended times on stream (~160 h). Two distinct regimes with different product distributions were observed for the Cu-PMO and CuNi-PMO catalyst with time on stream. At the start of the run, the initial conversion of ethanol dropped from about 85% to less than 20% after 60 h and acetaldehyde was the main product (regime 1). At prolonged times on stream (60–160 h), fairly constant low conversions of ethanol (14%) were observed and 1-butanol was the main product (regime 2). Performance of the monometallic Cu-PMO catalyst in terms of 1-butanol yield and stability was lower compared to the bi-metallic CuNi-PMO. Detailed catalyst characterization studies (XRD, H2-TPR, sorption of acrylic acid, TGA, TEM, HAADF-STEM, and EDS mapping) on both fresh and spent CuNi-PMO taken at various times on stream was performed to determine the changes in catalyst morphology and composition during a run, and particularly to obtain information on changes in catalyst structure operating in regime 1 or 2. The change in chemoselectivity is in line with an increase in basicity of the catalyst at extended runtimes.

Recent trends in biobutanol production

Finite availability of conventional fossil carbonaceous fuels coupled with increasing pollution due to their overexploitation has necessitated the quest for renewable fuels. Consequently, biomass-derived fuels are gaining importance due to their economic viability and environment-friendly nature. Among various liquid biofuels, biobutanol is being considered as a suitable and sustainable alternative to gasoline. This paper reviews the present state of the preprocessing of the feedstock, biobutanol production through fermentation and separation processes. Low butanol yield and its toxicity are the major bottlenecks. The use of metabolic engineering and integrated fermentation and product recovery techniques has the potential to overcome these challenges. The application of different nanocatalysts to overcome the existing challenges in the biobutanol field is gaining much interest. For the sustainable production of biobutanol, algae, a third-generation feedstock has also been evaluated.

MgO Dispersed on Activated Carbon as Water Tolerant Catalyst for the Conversion of Ethanol into Butanol

MgO supported on activated carbon (AC) with a load ranging from 10% to 30% has been investigated as catalyst for the conversion of ethanol into butanol at 400 °C in a fixed bed reactor at different GHSV. Catalysts have been characterized by XRD, SEM/EDX, and N2 physisorption at 77 K. The high dispersion of MgO into the pores of the support provides strongly enhanced performance with respect to bulk MgO. MgO/AC catalysts have been also tested under wet feed conditions showing high water tolerance and significantly larger butanol yield with respect to an alumina supported Ru/MgO catalyst. After wet operation, the increased surface area of the catalyst leads to better performance once dry feed conditions are restored.

Biological Detoxification of Lignocellulosic Hydrolysates for Improved Biobutanol Production

The fuel butanol yield and productivity obtained during fermentation of lignocellulosic hydrolysates is decreased due to the presence of inhibiting compounds, such as carboxylic acids, phenolic compounds and furans formed during hydrolysis. The main objective of this work is to evaluate the biological detoxification method of lignocellulosic hydrolysates when two forms of specially adapted activated sludge are used for improved biobutanol production. We investigated the removal of toxic substances from an enzymatic hydrolysate of miscanthus cellulose and an acid hydrolysate of spruce by specially adapted activated sludge of the urban wastewater treatment plants and by activated sludge of the pig farm wastewater treatment plants. Activated sludge treatment removed 98 % and 99 % of 5-hydroxymethylfurfural (5-HMF) and furfural from the hydrolysate respectively. Fermentation of treated hydrolysates by Clostridium acetobutylicum ATCC 824 was successful. Control experiments on fermentation of untreated hydrolysates showed a complete absence of fermentation

Development and application of a novel screening method and experimental use of the mutant bacterial strain Clostridium beijerinckii NCIMB 8052 for production of butanol via fermentation of fresh cassava

Classic chemical mutagenesis has a demonstrated potential to create a strain ofClostridiumwith improved fermentation performance for obtaining high butanol yield.