Proteome Reference Map and Comparative Proteomic Analysis between a Wild TypeClostridium acetobutylicumDSM 1731 and its Mutant with Enhanced Butanol Tolerance and Butanol Yield

2010 ◽  
Vol 9 (6) ◽  
pp. 3046-3061 ◽  
Author(s):  
Shaoming Mao ◽  
Yuanming Luo ◽  
Tianrui Zhang ◽  
Jinshan Li ◽  
Guanhui Bao ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Butanol Tolerance ◽  
Comparative Proteomic Analysis ◽  
Reference Map ◽  
Butanol Yield

Reference map and comparative proteomic analysis of Neisseria gonorrhoeae displaying high resistance against spectinomycin

2014 ◽  
Vol 63 (3) ◽  
pp. 371-385 ◽  
Author(s):  
Sunanta Nabu ◽  
Ratana Lawung ◽  
Patcharee Isarankura-Na-Ayudhya ◽  
Chartchalerm Isarankura-Na-Ayudhya ◽  
Sittiruk Roytrakul ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Neisseria Gonorrhoeae ◽  
Protein Expression ◽  
Proteomic Analysis ◽  
Cell Envelope ◽  
Expression Profiles ◽  
Compensatory Mechanisms ◽  
Two Dimensional Gel Electrophoresis ◽  
Comparative Proteomic Analysis ◽  
Reference Map

A proteome reference map of Neisseria gonorrhoeae was successfully established using two-dimensional gel electrophoresis in conjunction with matrix-assisted laser desorption ionization–time of flight mass spectrometry. This map was further applied to compare protein expression profiles of high-level spectinomycin-resistant (clinical isolate) and -susceptible (reference strain) N. gonorrhoeae following treatment with subminimal inhibitory concentrations (subMICs) of spectinomycin. Approximately 200 protein spots were visualized by Coomassie brilliant blue G-250 staining and 66 spots representing 58 unique proteins were subsequently identified. Most of the identified proteins were analysed as cytoplasmic proteins and belonged to the class of energy metabolism. Comparative proteomic analysis of whole protein expression of susceptible and resistant gonococci showed up to 96 % similarity while eight proteins were found to be differentially expressed in the resistant strain. In the presence of subMICs of spectinomycin, it was found that 50S ribosomal protein L7/L12, an essential component for ribosomal translocation, was upregulated in both strains, ranging from 1.5- to 3.5-fold, suggesting compensatory mechanisms of N. gonorrhoeae in response to antibiotic that inhibits protein synthesis. Moreover, the differential expression of proteins involved in energy metabolism, amino acid biosynthesis, and the cell envelope was noticeably detected, indicating significant cellular responses and adaptation against antibiotic stress. Such knowledge provides valuable data, not only fundamental proteomic data, but also knowledge of the mode of action of antibiotic and secondary target proteins implicated in adaptation and compensatory mechanisms.

scholarly journals Proteome Profiling of PMJ2-R and Primary Peritoneal Macrophages

2021 ◽  
Vol 22 (12) ◽  
pp. 6323
Author(s):  
Alexander L. Rusanov ◽  
Peter M. Kozhin ◽  
Olga V. Tikhonova ◽  
Victor G. Zgoda ◽  
Dmitry S. Loginov ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Living Organism ◽  
Peritoneal Macrophages ◽  
In Vitro Models ◽  
Individual Characteristics ◽  
Essential Proteins ◽  
Comparative Proteomic Analysis ◽  
Immortalized Cell ◽  
The Individual

In vitro models are often used for studying macrophage functions, including the process of phagocytosis. The application of primary macrophages has limitations associated with the individual characteristics of animals, which can lead to insufficient standardization and higher variability of the obtained results. Immortalized cell lines do not have these disadvantages, but their responses to various signals can differ from those of the living organism. In the present study, a comparative proteomic analysis of immortalized PMJ2-R cell line and primary peritoneal macrophages isolated from C57BL/6 mice was performed. A total of 4005 proteins were identified, of which 797 were quantified. Obtained results indicate significant differences in the abundances of many proteins, including essential proteins associated with the process of phagocytosis, such as Elmo1, Gsn, Hspa8, Itgb1, Ncf2, Rac2, Rack1, Sirpa, Sod1, C3, and Msr1. These findings indicate that outcomes of studies utilizing PMJ2-R cells as a model of peritoneal macrophages should be carefully validated. All MS data are deposited in ProteomeXchange with the identifier PXD022133.

scholarly journals Comparative proteomic analysis of mitochondria isolated from Euglena gracilis under aerobic and hypoxic conditions

PLoS ONE ◽  
2019 ◽  
Vol 14 (12) ◽  
pp. e0227226 ◽  
Author(s):  
Shun Tamaki ◽  
Kohei Nishino ◽  
Takahisa Ogawa ◽  
Takanori Maruta ◽  
Yoshihiro Sawa ◽  
...  
Keyword(s):  
Euglena Gracilis ◽  
Proteomic Analysis ◽  
Comparative Proteomic Analysis ◽  
Hypoxic Conditions

Comparative proteomic analysis of whole saliva from chronic periodontitis patients

2010 ◽  
Vol 73 (7) ◽  
pp. 1334-1341 ◽  
Author(s):  
Lorena Da Rós Gonçalves ◽  
Márcia Regina Soares ◽  
Fábio C.S. Nogueira ◽  
Carlos Garcia ◽  
Danielle Resende Camisasca ◽  
...  
Keyword(s):  
Chronic Periodontitis ◽  
Proteomic Analysis ◽  
Comparative Proteomic Analysis ◽  
Whole Saliva

Comparative Proteomic Analysis of Indioside D-Triggered Cell Death in HeLa Cells

2008 ◽  
Vol 7 (5) ◽  
pp. 2050-2058 ◽  
Author(s):  
Chi Chun Wong ◽  
Ying Wang ◽  
Ka-Wing Cheng ◽  
Jen-Fu Chiu ◽  
Qing-Yu He ◽  
...  
Keyword(s):  
Cell Death ◽  
Hela Cells ◽  
Proteomic Analysis ◽  
Comparative Proteomic Analysis
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