adsorbed protein layer
Recently Published Documents


TOTAL DOCUMENTS

8
(FIVE YEARS 0)

H-INDEX

4
(FIVE YEARS 0)

2020 ◽  
Vol 6 (5) ◽  
pp. eaay2756 ◽  
Author(s):  
Qihui Zhou ◽  
Jiawen Chen ◽  
Yafei Luan ◽  
Petteri A. Vainikka ◽  
Sebastian Thallmair ◽  
...  

Artificial rotary molecular motors convert energy into controlled motion and drive a system out of equilibrium with molecular precision. The molecular motion is harnessed to mediate the adsorbed protein layer and then ultimately to direct the fate of human bone marrow–derived mesenchymal stem cells (hBM-MSCs). When influenced by the rotary motion of light-driven molecular motors grafted on surfaces, the adsorbed protein layer primes hBM-MSCs to differentiate into osteoblasts, while without rotation, multipotency is better maintained. We have shown that the signaling effects of the molecular motion are mediated by the adsorbed cell-instructing protein layer, influencing the focal adhesion–cytoskeleton actin transduction pathway and regulating the protein and gene expression of hBM-MSCs. This unique molecular-based platform paves the way for implementation of dynamic interfaces for stem cell control and provides an opportunity for novel dynamic biomaterial engineering for clinical applications.


2015 ◽  
Vol 33 (11) ◽  
pp. 1516-1526 ◽  
Author(s):  
Zhen-bing Li ◽  
Yan-hui Xiang ◽  
Xian-jing Zhou ◽  
Jing-jing Nie ◽  
Mao Peng ◽  
...  

2010 ◽  
Vol 1277 ◽  
Author(s):  
Phaedra Silva-Bermudez ◽  
Sandra E. Rodil

The adsorption of bovine serum albumin (BSA) and fibrinogen proteins dissolved on Phosphate buffer solution onto Ta, Nb and Ti oxide thin films was studied. The metal oxide thin films were deposited by magnetron sputtering on Si(100) wafers and characterized by contact angle measurements and profilometry. Spectroscopic ellipsometry was employed to characterize the kinetics of the protein adsorption process in-situ at the solid-liquid interface and the optical properties of the adsorbed protein layer formed after 45 minutes of immersion of the thin film in the protein solution. Infrared spectroscopy was used to study the proteins within the adsorbed layer. A trend indicating that the surface mass density of the adsorbed protein layer increases as the Rt (peak-to-valley height) surface roughness parameter increases was observed for fibrinogen and BSA. An increment in the surface mass density of the adsorbed protein layer was also observed onto surfaces with higher polar components of the surface energy. BSA and fibrinogen seemed to more readily adsorbed onto tantalum oxide than onto titanium oxide.


1990 ◽  
Vol 268 (11) ◽  
pp. 1036-1043 ◽  
Author(s):  
H. J. Holterman ◽  
E. J. 's-Gravenmade ◽  
H. A. Waterman ◽  
J. Mellema ◽  
C. Blom

Sign in / Sign up

Export Citation Format

Share Document