scholarly journals Unidirectional rotating molecular motors dynamically interact with adsorbed proteins to direct the fate of mesenchymal stem cells

2020 ◽  
Vol 6 (5) ◽  
pp. eaay2756 ◽  
Author(s):  
Qihui Zhou ◽  
Jiawen Chen ◽  
Yafei Luan ◽  
Petteri A. Vainikka ◽  
Sebastian Thallmair ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Molecular Motors ◽  
Molecular Motion ◽  
Transduction Pathway ◽  
Protein Layer ◽  
Adsorbed Protein ◽  
Adsorbed Proteins ◽  
Adsorbed Protein Layer ◽  
Controlled Motion

Artificial rotary molecular motors convert energy into controlled motion and drive a system out of equilibrium with molecular precision. The molecular motion is harnessed to mediate the adsorbed protein layer and then ultimately to direct the fate of human bone marrow–derived mesenchymal stem cells (hBM-MSCs). When influenced by the rotary motion of light-driven molecular motors grafted on surfaces, the adsorbed protein layer primes hBM-MSCs to differentiate into osteoblasts, while without rotation, multipotency is better maintained. We have shown that the signaling effects of the molecular motion are mediated by the adsorbed cell-instructing protein layer, influencing the focal adhesion–cytoskeleton actin transduction pathway and regulating the protein and gene expression of hBM-MSCs. This unique molecular-based platform paves the way for implementation of dynamic interfaces for stem cell control and provides an opportunity for novel dynamic biomaterial engineering for clinical applications.

scholarly journals Bone Marrow Mesenchymal Stem Cells (BM-MSCs) Improve Heart Function in Swine Myocardial Infarction Model through Paracrine Effects

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Min Cai ◽  
Rui Shen ◽  
Lei Song ◽  
Minjie Lu ◽  
Jianguang Wang ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Signal Transduction ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Glucose Metabolism ◽  
Signal Transduction Pathway ◽  
Transduction Pathway ◽  
Pet Ct ◽  
Marrow Mesenchymal Stem Cells

Abstract Stem cells are promising for the treatment of myocardial infarction (MI) and large animal models should be used to better understand the full spectrum of stem cell actions and preclinical evidences. In this study, bone marrow mesenchymal stem cells (BM-MSCs) were transplanted into swine heart ischemia model. To detect glucose metabolism in global left ventricular myocardium and regional myocardium, combined with assessment of cardiac function, positron emission tomography-computer tomography (PET-CT) and magnetic resonance imaging (MRI) were performed. To study the changes of glucose transporters and glucose metabolism-related enzymes and the signal transduction pathway, RT-PCR, Western-blot, and immunohistochemistry were carried out. Myocardium metabolic evaluation by PET-CT showed that mean signal intensity (MSI) increased in these segments at week 4 compared with that at week 1 after BM-MSCs transplantation. Moreover, MRI demonstrated significant function enhancement in BM-MSCs group. The gene expressions of glucose transporters (GLUT1, GLUT4), glucose metabolism-related enzymes phosphofructokinase (PFK), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH)) and 70-kDa ribosomal protein S6 kinase (p70s6k) in BM-MSCs injected areas were up-regulated at week 4 after BM-MSCs transplantation and this was confirmed by Western-blot and immunohistochemistry. In conclusions, BM-MSCs transplantation could improve cardiac function in swine MI model by activation of mTOR signal transduction pathway.

scholarly journals Thermo-sensitive poly(DEGMMA-co-MEA) microgels: Synthesis, characterization and interfacial interaction with adsorbed protein layer

2015 ◽  
Vol 33 (11) ◽  
pp. 1516-1526 ◽  
Author(s):  
Zhen-bing Li ◽  
Yan-hui Xiang ◽  
Xian-jing Zhou ◽  
Jing-jing Nie ◽  
Mao Peng ◽  
...  
Keyword(s):  
Interfacial Interaction ◽  
Protein Layer ◽  
Adsorbed Protein ◽  
Adsorbed Protein Layer

Protein Adsorption on Amorphous Metal Oxide Thin Films: An FTIR/ATR and Ellipsometry study

2010 ◽  
Vol 1277 ◽  
Author(s):  
Phaedra Silva-Bermudez ◽  
Sandra E. Rodil
Keyword(s):  
Thin Films ◽  
Protein Adsorption ◽  
Mass Density ◽  
Oxide Thin Films ◽  
Protein Layer ◽  
Metal Oxide Thin Films ◽  
Surface Mass ◽  
Adsorbed Protein ◽  
Surface Mass Density ◽  
Adsorbed Protein Layer

The adsorption of bovine serum albumin (BSA) and fibrinogen proteins dissolved on Phosphate buffer solution onto Ta, Nb and Ti oxide thin films was studied. The metal oxide thin films were deposited by magnetron sputtering on Si(100) wafers and characterized by contact angle measurements and profilometry. Spectroscopic ellipsometry was employed to characterize the kinetics of the protein adsorption process in-situ at the solid-liquid interface and the optical properties of the adsorbed protein layer formed after 45 minutes of immersion of the thin film in the protein solution. Infrared spectroscopy was used to study the proteins within the adsorbed layer. A trend indicating that the surface mass density of the adsorbed protein layer increases as the Rt (peak-to-valley height) surface roughness parameter increases was observed for fibrinogen and BSA. An increment in the surface mass density of the adsorbed protein layer was also observed onto surfaces with higher polar components of the surface energy. BSA and fibrinogen seemed to more readily adsorbed onto tantalum oxide than onto titanium oxide.

scholarly journals Flow curves of an adsorbed protein layer at the saliva-air interface

1990 ◽  
Vol 268 (11) ◽  
pp. 1036-1043 ◽  
Author(s):  
H. J. Holterman ◽  
E. J. 's-Gravenmade ◽  
H. A. Waterman ◽  
J. Mellema ◽  
C. Blom
Keyword(s):  
Protein Layer ◽  
Flow Curves ◽  
Adsorbed Protein ◽  
Air Interface ◽  
Adsorbed Protein Layer

Proliferation and multi-differentiation potentials of human mesenchymal stem cells on thermoresponsive PDMS surfaces grafted with PNIPAAm

2010 ◽  
Vol 30 (6) ◽  
pp. 455-455 ◽  
Author(s):  
Dongyan Shi ◽  
Dan Ma ◽  
Feiqing Dong ◽  
Chen Zong ◽  
Liyue Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells
Sign in / Sign up

Export Citation Format

Share Document