Partial characterization of the adsorbed protein layer onDirofilaria immitis (Nematoda) cuticle

1989 ◽  
Vol 75 (7) ◽  
pp. 554-558 ◽  
Author(s):  
A. K. Kadipasaoglu ◽  
F. H. Bilge
Keyword(s):  
Partial Characterization ◽  
Protein Layer ◽  
Adsorbed Protein ◽  
Adsorbed Protein Layer

Control and Characterization of Protein Adsorption on Ceramic Surfaces

1999 ◽  
Vol 599 ◽  
Author(s):  
M. J. Read ◽  
S. L. Burkett ◽  
A. M. Mayes
Keyword(s):  
Protein Adsorption ◽  
Conformational Changes ◽  
Surface Composition ◽  
Biological Properties ◽  
Protein Layer ◽  
Adsorption Mechanisms ◽  
Adsorbed Protein ◽  
Ceramic Surfaces ◽  
Model Protein

AbstractProtein adsorption to ceramic surfaces is an important early step in the function of implants. The types and amounts of adsorbed protein and the resulting conformational changes could mediate subsequent cell adhesion and inorganic deposition. Microporous silicoalumino-phosphates, which allow variations in surface composition within the same crystal structure, have been used as model surfaces. Effects of surface composition on adsorption isotherms, elutability, and biological activity of the adsorbed protein layer have been studied using lysozyme, a model protein. Control over protein adsorption mechanisms using well-characterized surface properties could be used to predict the biological properties of surfaces, and engineer coatings for a desired response.

scholarly journals Thermo-sensitive poly(DEGMMA-co-MEA) microgels: Synthesis, characterization and interfacial interaction with adsorbed protein layer

2015 ◽  
Vol 33 (11) ◽  
pp. 1516-1526 ◽  
Author(s):  
Zhen-bing Li ◽  
Yan-hui Xiang ◽  
Xian-jing Zhou ◽  
Jing-jing Nie ◽  
Mao Peng ◽  
...  
Keyword(s):  
Interfacial Interaction ◽  
Protein Layer ◽  
Adsorbed Protein ◽  
Adsorbed Protein Layer

Protein Adsorption on Amorphous Metal Oxide Thin Films: An FTIR/ATR and Ellipsometry study

2010 ◽  
Vol 1277 ◽  
Author(s):  
Phaedra Silva-Bermudez ◽  
Sandra E. Rodil
Keyword(s):  
Thin Films ◽  
Protein Adsorption ◽  
Mass Density ◽  
Oxide Thin Films ◽  
Protein Layer ◽  
Metal Oxide Thin Films ◽  
Surface Mass ◽  
Adsorbed Protein ◽  
Surface Mass Density ◽  
Adsorbed Protein Layer

The adsorption of bovine serum albumin (BSA) and fibrinogen proteins dissolved on Phosphate buffer solution onto Ta, Nb and Ti oxide thin films was studied. The metal oxide thin films were deposited by magnetron sputtering on Si(100) wafers and characterized by contact angle measurements and profilometry. Spectroscopic ellipsometry was employed to characterize the kinetics of the protein adsorption process in-situ at the solid-liquid interface and the optical properties of the adsorbed protein layer formed after 45 minutes of immersion of the thin film in the protein solution. Infrared spectroscopy was used to study the proteins within the adsorbed layer. A trend indicating that the surface mass density of the adsorbed protein layer increases as the Rt (peak-to-valley height) surface roughness parameter increases was observed for fibrinogen and BSA. An increment in the surface mass density of the adsorbed protein layer was also observed onto surfaces with higher polar components of the surface energy. BSA and fibrinogen seemed to more readily adsorbed onto tantalum oxide than onto titanium oxide.

scholarly journals Flow curves of an adsorbed protein layer at the saliva-air interface

1990 ◽  
Vol 268 (11) ◽  
pp. 1036-1043 ◽  
Author(s):  
H. J. Holterman ◽  
E. J. 's-Gravenmade ◽  
H. A. Waterman ◽  
J. Mellema ◽  
C. Blom
Keyword(s):  
Protein Layer ◽  
Flow Curves ◽  
Adsorbed Protein ◽  
Air Interface ◽  
Adsorbed Protein Layer

scholarly journals Unidirectional rotating molecular motors dynamically interact with adsorbed proteins to direct the fate of mesenchymal stem cells

2020 ◽  
Vol 6 (5) ◽  
pp. eaay2756 ◽  
Author(s):  
Qihui Zhou ◽  
Jiawen Chen ◽  
Yafei Luan ◽  
Petteri A. Vainikka ◽  
Sebastian Thallmair ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Molecular Motors ◽  
Molecular Motion ◽  
Transduction Pathway ◽  
Protein Layer ◽  
Adsorbed Protein ◽  
Adsorbed Proteins ◽  
Adsorbed Protein Layer ◽  
Controlled Motion

Artificial rotary molecular motors convert energy into controlled motion and drive a system out of equilibrium with molecular precision. The molecular motion is harnessed to mediate the adsorbed protein layer and then ultimately to direct the fate of human bone marrow–derived mesenchymal stem cells (hBM-MSCs). When influenced by the rotary motion of light-driven molecular motors grafted on surfaces, the adsorbed protein layer primes hBM-MSCs to differentiate into osteoblasts, while without rotation, multipotency is better maintained. We have shown that the signaling effects of the molecular motion are mediated by the adsorbed cell-instructing protein layer, influencing the focal adhesion–cytoskeleton actin transduction pathway and regulating the protein and gene expression of hBM-MSCs. This unique molecular-based platform paves the way for implementation of dynamic interfaces for stem cell control and provides an opportunity for novel dynamic biomaterial engineering for clinical applications.

Partial characterization of the N-linked oligosaccharides occurring on rat hepatocyte glycoproteins

2010 ◽  
Vol 108 (10) ◽  
pp. 323-329 ◽  
Author(s):  
Marti F. A. Bierhuizen ◽  
Moniek de Wit ◽  
Carin A. R. L. Govers ◽  
Willem van Dijk
Keyword(s):  
Partial Characterization ◽  
Rat Hepatocyte
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