Nitrous oxide emissions from denitrification and the partitioning of gaseous losses as affected by nitrate and carbon addition and soil aeration

National inventories of N2O emissions from agricultural situations are being developed; however, the factors controlling such emissions may vary with soil and environmental conditions and management practices. This study determined the relative importance of soil aeration, as measured by water-filled pore space (WFPS), NO3− addition, C addition and C source on the amount and partitioning of gaseous N losses from denitrification from an arable soil in Atlantic Canada. Denitrification (N2O + N2) and N2O emissions were measured on repacked soil cores using acetylene inhibition.The N2O:(N2O + N2) ratio was frequently 0.7 or higher, indicating that most emissions occurred as N2O. N2O emissions and denitrification were negligible at a WFPS of 0.45 m3 m-3, and high at WFPS of 0.75 m3 m-3, regardless of NO3−or C addition treatments. At a WFPS of 0.60 m3 m-3, N2O emissions and denitrification were low and were increased by both NO3− and C addition treatments. Carbon source was investigated by amendment with glucose, red clover or barley straw. Based on the quantity of soil respiration per unit of C added in the amendment, C in the red clover and barley straw was estimated to be 48 and 28% as available as glucose C. When corrected for C availability, cumulative N2O emissions averaged 0.010, 0.011 and 0.002 mg N kg-1 soil, and cumulative denitrification averaged 0.014, 0.014 and 0.003 mg N kg-1 soil, for each 1.0 mg C kg-1 soil of available C added as glucose, red clover or barley straw, respectively. NO3− addition had no effect on denitrification, but increased N2O emissions, especially where C availability was high. The amount of denitrification was controlled primarily by soil O2 supply, as controlled by WFPS and C availability. The N2O:(N2O + N2) ratio was generally high in cases where the supply of O2 or NO3− was sufficient to meet the demand for terminal electron acceptors. Key words: Denitrification, nitrous oxide, glucose, red clover, barley straw, carbon availability, terminal electron acceptor, aeration

Water-filled microbially habitable pores: Relation to denitrification

The onset of a decline in net N mineralization, primarily due to denitrification, has been related to water contents, θW, or water-filled porosities, fW, but values of these characteristics vary among soils thus limiting their use as diagnostic criteria. An implicit assumption in the use of these characteristics is that water-filled pores of all sizes are habitable by microorganisms participating in denitrification. However, microorganisms are excluded from small pores, and the volume fraction of these pores varies with soil structure. The objective of this study was to determine if variation in the volume fraction of water-filled microbially habitable pores, θMHP, contributes to the variation in denitrification in soils of different structure. Data were used from studies with and without growing maize (Zea maize L.) plants. Variation in soil structure was achieved by using soils of different texture and organic carbon contents that were packed to two different levels of relative compaction. At the onset of a decrease in net N mineralization, values of θMHP exhibited less variability among soils than either θW or fW. The θMHP will be of greatest value as a diagnostic criterion for the decline in net N mineralization in soils exhibiting variation in the volume fraction of pores ≤ 4 µm diameter. Key words: Denitrification, habitable pore space, soil structure

Seasonal response of denitrifiers to temperature in a Quebec cropped soil

While some studies indicate no denitrification activity in early spring, others have demonstrated that denitrifiers from temperate region soils can adapt to low temperatures. The aim of the present paper was to study how seasonal changes in temperature affect denitrifying enzyme activity (DEA) in a cropped humic gleysol located in a cold temperate climate (Quebec). Soil was sampled monthly during a 16-mo period and DEA was measured at nine temperatures from 2 to 35°C. A seasonal effect of temperature on DEA was significant at all incubation temperatures and was more important in November and in May–June. The effect of temperature on DEA was better fitted with the square root model of Ratkowsky than with the Arrhenius equation. The regression coefficient b (Ratkowsky parameter) varied seasonally with a trend similar to that of DEA. These results show that the Ratkowsky model should be used instead of Arrhenius equation to describe the effect of cold temperature on denitrification. Key words: Denitrification, temperature, cold, Arrhenius equation, Ratkowsky model

Denitrification and nitrous oxide emissions from a Black Chernozemic soil during spring thaw in Alberta

Previous field research in Alberta has suggested that denitrification occurs mostly when soil thaws in the spring, with associated soil water saturation. Our objective was to determine if denitrification and N2O emission in fact take place in cold, thawing soil in the field. Denitrification and N2O flux were measured in two springs and the intervening summer. Cylinders were placed in soil in November, 1988, and 57 kg N ha−1 of 15Nlabeled KNO3 was added. Soil 15N mass balance technique showed 23 kg N ha−1 of added-N was lost by 15 May 1989. Gas trappings were made (28 March to 29 April) and nearly all of the N2O emission (3.5 kg N2O-N ha−1) occurred during an 11-d period of thaw. The accumulated N2O flux from 20 June to 31 August was small (0.5 kg N2O-N ha−1, or less); during that time there were no rainfall events intense enough to produce water saturated soil. In 1990, 15N-labeled KNO3 (100 kg N ha−1) was applied on 26 March (outset of the thaw) and mass balance showed 32.7 kg N ha−1of added-N was lost by 7 May. A flux of 16.3 kg N2O-N ha−1 occurred largely in a 10-d period during and immediately after soil thaw. The N2O emitted from soil left a considerable fraction of the lost N unaccounted for. This unaccounted N was most likely lost as gaseous N other than N2O (e.g., N2). We conclude that large amounts of soil nitrate may be denitrified, with smaller amounts emitted as N2O, as the soil thaws and soon thereafter. Key words: Denitrification, frozen soil, thawing soil, nitrogen, nitrous oxide

Nitrous oxide emission from agricultural soils

A review of the salient features of N2O emissions from agricultural soils was done to assess our current understanding and associated problems. Nitrous oxide is an important globe warming gas and a destructive agent of ozone in the stratosphere. A major concern is the increasing contribution of chemical fertilizers to atmospheric N2O buildup. There is only a limited understanding of the contributions from manures, biological N2 fixation and crop residues. A recent estimate suggests that agriculture's share of N2O emissions is 80% although such estimates are highly uncertain because of imprecise data and the physical and biological complexities of the production process. As a product of the nitrification and denitrification process in soils, a major problem is our understanding of the proportion of N2O produced, i.e. the product ratios, although there is a good general understanding of the processes involved. Measurements of N2O emissions from the soil surface fail to take into account N2O flux from the bottom of the root zone into the subsoil and aquifers although they are generally considered to be significant. There is a need to apply newly available methodology and for combining this methodology and modelling together to predict N2O emissions on the landscape (or field) scale taking climate, soil and cropping variables into account. There is enough information available now to exercise some control of N2O emissions from cultivated soils. It is suggested that this be done focusing on factors that directly affect the soil microbes involved with the nitrification (NH4+, O2) and denitrification (NO3−, C, O2) processes. Cropping practices and some soil characteristic amendments are suggested herein for this purpose. Key words: Denitrification, nitrification, emission control, gas ratios

Denitrification following herbicide application to a grass sward

The application of the herbicide Roundup (glyphosate), and subsequent death of a predominately bromegrass (Bromus inermis Leyss.) and blue grass (Poa pratensis L.) sward, resulted in a 20- to 30-fold increase in denitrification rate 14 and 49 d after application compared to herbicide-untreated and fallowed soil treatments. The regulation of denitrification by O2, carbon and NO3− availabilities was assessed by measurement of various soil variables. The regulation of denitrification by C and NO3− availabilities was further studied in a laboratory experiment in which denitrification was measured following NO3− and glucose-C addition to soil from the field treatments. Elevated denitrification in the herbicide-treated soil was attributed to increased soil moisture and NO3− contents resulting from the death of vegetation. The death of the grass sward did not increase available C to denitrifiers, whereas the absence of vegetation in the fallowed soil 1 yr following herbicide application reduced available C. This study indicates that herbicide application to a grass sward increases denitrification and hence may contribute to greater nitrous oxide emission and N loss from soil. Key words: Denitrification, herbicide, plants, nitrate, regulation, soil moisture

An empirical model of denitrification

We used a simple empirical model to predict denitrification rates from measurements of bulk soil properties. Boundary analysis was used to define relationships between denitrification rate and each of air-filled porosity, respiration rate and mineralizable-C content. The ratio of measured denitrifying enzyme activity to the maximum measured value was used to account for variation in amounts of enzymes and numbers of denitrifiers in soil. Nitrate content had little effect on denitrification rate and was excluded from the model. Because the model did not account for microscale variability, it did not accurately predict rates in individual soil cores. Nevertheless, population means and distributions of predicted and measured values were similar. The seasonal patterns of mean values of predicted and measured denitrification rates were also similar over the second half of the sampling period, which extended from May to November. The model did not account for appreciable denitrification on three dates in May. This discrepancy indicated that environmental regulation of denitrification may not be uniform over the season. The model was not sufficiently sensitive to factors influencing episodic events. Key words: Denitrification rate, model, boundary line

Sulfide alleviation of acetylene inhibition of nitrous oxide reduction by Flexibacter Canadensis

The role of sulfide in the relief of acetylene inhibition of nitrous oxide reduction by Flexibacter canadensis was studied. In this organism, the reversal of acetylene inhibition of nitrous oxide reduction is correlated with a 90% decrease in the dissolved sulfide concentration. The fate of this sulfide is not known, since there was no concomitant increase in acid-soluble sulfide and volatile sulfur compounds were not detectable by flame photometric gas chromatography. Of the other sulfur-containing compounds tested (sulfate, sulfite, thiosulfate, cysteine, methionine, dithionite, dithionate, and glutathione), only cysteine relieved the acetylene block of nitrous oxide reduction by F. canadensis. Under similar experimental conditions, other denitrifiers tested (Azospirillum brasilense, Pseudomonas stutzeri, and a Flavobacterium isolate) failed to reduce nitrous oxide in the presence of sulfide and an inhibitory concentration of acetylene. It is concluded that both biological and abiological factors contribute to the sulfide relief of acetylene inhibition of nitrous oxide by pure cultures of F. canadensis. Key words: denitrification, nitrous oxide, acetylene, sulfide, Flexibacter canadensis.

Quantifying denitrification on a field scale in hummocky terrain

In this study a landscape classification and daily climatic data were used to extrapolate from a number of discrete denitrification rate measurements to an annual field average. Denitrification rates were calculated from modelled daily moisture contents and observed daily temperature using regression equations. Different regression equations were used for crop and fallowed fields, and for three distinct landscape groups; depositional, intermediate, and erosional. Good correlations were found between predicted and measured denitrification rates. Annual denitrification losses were greatest under fallow, ranging from 50.5 kg N ha−1 on the low level landscape element to 3.1 kg N ha−1 on the diverging blackslope. Losses from cropped soil ranged from 12.8 kg N ha−1 on the diverging footslope in 1988 to 0.7 kg N ha−1 on the diverging backslope in 1986. Field average denitrification losses were estimated to be 3 kg N ha−1 in 1986 (wheat), 16 kg N ha−1 in 1987 (the fallow year) and 4 kg N ha−1 in 1988 (canola). Key words: Denitrification, annual field averages, landscape elements, soil properties, simulation model

EFFECT OF WHEAT STRAW INCORPORATION ON DENITRIFICATION OF N UNDER ANAEROBIC AND AEROBIC CONDITIONS

The effects of wheat straw incorporation on denitrification, immobilization of N, and C mineralization were investigated at H2O contents of 60, 90 and 120% saturation. Incorporation of increasing levels of straw consistently increased the rate of denitrification for the first 4–8 d, followed by negligible N losses thereafter. In a total period of 96 d, the addition of 1.0% straw increased N losses from 2.5 to 10.1, and from 61.6 to 83.9 μg g−1 in the 60 and 120% water saturation treatments, respectively. The pattern of CO2-C evolved was practically identical to that of the denitrification rate for the initial period when sufficient [Formula: see text] was present. This study has confirmed that in flooded soils, high rates of denitrification will persist only when C is supplied by native or applied organic C sources, provided adequate [Formula: see text] is present. When [Formula: see text] was low, denitrification rates rapidly decreased, even with a sufficient supply of C. Immobilization of fertilizer N (50 μg N g−1 as K15NO3) was very rapid. Around 90% of the total immobilization of applied N occurred within 4 d. Incorporation of 1.0% straw increased the immobilization of fertilizer N from 8.4 to 42.8, and from 1.0 to 7.6% in the 60 and 120% water-saturated treatments, respectively. Remineralization of recently immobilized fertilizer N was observed after 32 d in the 60% saturation treatments only. Key words: Denitrification, wheat straw, mineralization of N