Denitrification following herbicide application to a grass sward
The application of the herbicide Roundup (glyphosate), and subsequent death of a predominately bromegrass (Bromus inermis Leyss.) and blue grass (Poa pratensis L.) sward, resulted in a 20- to 30-fold increase in denitrification rate 14 and 49 d after application compared to herbicide-untreated and fallowed soil treatments. The regulation of denitrification by O2, carbon and NO3− availabilities was assessed by measurement of various soil variables. The regulation of denitrification by C and NO3− availabilities was further studied in a laboratory experiment in which denitrification was measured following NO3− and glucose-C addition to soil from the field treatments. Elevated denitrification in the herbicide-treated soil was attributed to increased soil moisture and NO3− contents resulting from the death of vegetation. The death of the grass sward did not increase available C to denitrifiers, whereas the absence of vegetation in the fallowed soil 1 yr following herbicide application reduced available C. This study indicates that herbicide application to a grass sward increases denitrification and hence may contribute to greater nitrous oxide emission and N loss from soil. Key words: Denitrification, herbicide, plants, nitrate, regulation, soil moisture