Genome Relationship among Nine Species of Millettieae (Leguminosae: Papilionoideae) Based on Random Amplified Polymorphic DNA (RAPD)

Random amplified polymorphic DNA (RAPD) marker was used to establish intergeneric classification and phylogeny of the tribe Millettieae sensu Geesink (1984) (Leguminosae: Papilionoideae) and to assess genetic relationship between 9 constituent species belonging to 5 traditionally recognized genera under the tribe. DNA from pooled leaf samples was isolated and RAPD analysis performed using 25 decamer primers. The genetic similarities were derived from the dendrogram constructed by the pooled RAPD data using a similarity index, which supported clear grouping of species under their respective genera, inter- and intra-generic classification and phylogeny and also merger of Pongamia with Millettia. Elevation of Tephrosia purpurea var. pumila to the rank of a species (T. pumila) based on morphological characteristics is also supported through this study of molecular markers.

Genome relationship between Thinopyrum bessarabicum and Thinopyrum elongatum

Two wheat/Thinopyrum amphiploids, wheat/T. bessarabicum and wheat/T. elongatum were crossed together and meiosis was studied in the F1 hybrid. The data show that although the wheat chromosomes pair fully, those of the two diploid Thinopyrum species rarely pair, 21 bivalents plus 14 univalents being the modal configuration. The genome symbols E for T. elongatum and Eb for T. bessarabicum are proposed.Key words: Thinopyrum elongatum, Thinopyrum bessarabicum, genome relationship.