The Effect of Water Mineralization on the Extraction of Active Compounds from Selected Herbs and on the Antioxidant Properties of the Obtained Brews

The objective of the study was to analyze the effect of total content of minerals in waters on the effectiveness of extraction of polyphenols and flavonoids and on the antioxidant properties of brews from leaves of green and black tea, leaves of peppermint, chamomile inflorescences, leaves of sage, and flowers of lavender. The process of brewing was conducted at an initial temperature of 95 °C, under cover, for 15 min, with the use of 10 waters differing in terms of mineral components. The content of total polyphenols and total flavonoids was determined in the brews obtained. The antioxidant properties were assayed with the use of stable DPPH radicals and Ferric Reducing Antioxidant Power (FRAP) reagent. A significant effect of water mineralization on the extraction of phenolic compounds and on the antioxidant properties of the brews obtained was observed. In the majority of cases, the highest concentrations of polyphenols and flavonoids were obtained in brews prepared with the use of deionized water and waters with medium levels of mineralization. It was also demonstrated that there was a significant reduction of antioxidant properties in brews prepared with the water that had the highest level of mineralization (2896 ppm), at 70% and 93%, respectively, for green and black teas.

Influence of water mineralization and dissolved main cations on survival, size and seismosensory characteristics of perch fry (Perca fluviatilis L.)

Within two months the growing of the perch fry Perca fluviatilis L. carried out in the aquarium experiment. Fertilized eggs of this fish species were taken from the spawning ground of a small and unpolluted natural reservoir and placed in laboratory conditions for subsequent incubation under different hydrochemical conditions. Experimental groups of perch were created in two replicates that developed under control conditions with a water salinity of 160 mg/l, under conditions of fresh water diluted to 100 mg/l, and under conditions with water whose salinity was increased relative to the control to 400 mg/l in three different ways - by adding calcium, magnesium or sodium chloride salts. Temperature, light, and oxygen conditions for growing fry in all groups were the same. The perch larvae were fed first with small natural zooplankton, then with artificially grown nauplia of Artemia; perch fry - with tubificid from vermiculture. The average survival rates of late larvae and early fry of perch in the second half of the experiment were maximal in the groups that developed under hydrochemical conditions with the addition of Na+ and Mg2+ (73% and 67%), and minimal in the groups that developed in the least mineralized water (46%). At the end of the experiment, the surviving fry from the groups affected by the increased content of sodium chloride had statistically significantly (p < 0.05) larger linear sizes than the fry from the control groups and groups that developed in the least mineralized water. Perch fry from the groups affected by increased concentrations of Ca2+ were characterized by a statistically significant (p < 0.05) smaller number of pores in the infraorbital and mandibular seismosensory canals of the head, compared with fry formed in conditions of least water mineralization and the minimal content of this main cation in it. Thus, salinity values and features of the ionic composition of fresh water can significantly affect the development of perch fry, which should be taken into account when breeding it in aquaculture.

The analysis of time series of river water mineralization in the Dnipro basin with the use of theoretical laws of random variables distribution

The analysis of current scientific work on the use of statistical methods in hydrochemical research has shown that this approach is sufficiently substantial, both in Ukraine and abroad. The purpose of this work is to determine the main statistical parameters and to research the possibility of applying theoretical laws of distribution to the time series of water mineralization.This research presents the results of the application of standard statistical methods of hydrometeorological information processing for data on water mineralization at 28 gauges of the Dnipro basin (within Ukraine) for the period from 1990 to 2015. The dynamics of the obtained statistical parameters (long-term annual average, coefficients of variation, asymmetry and autocorrelation) within the Dnipro basin in Ukraine has been analyzed. The average annual values of mineralization vary substantially within the studied part of the Dnipro basin - in the northern part the maximum value of the annual average mineralization is 447 mg/l, as it moves to the south, the mineralization increases and in the sub-basin of the Middle Dnipro it reaches a maximum of 971 mg/l; the highest values are observed in the south (sub-basin of the Lower Dnipro), where they can reach extremely high values for particular small rivers (the Solon River - Novopavlivka village, 3356 mg / l). The long-term variability of mineralization in the rivers of the studied area is insignificant, and the autocorrelation coefficients of the mineralization series are quite high, in most cases they are significant and tend to decrease from the sub-basin of the Prypyat’ river in the north to the sub-basin of the Lower Dnipro river in the south. Within the framework of the presented research, the possibility of using theoretical distribution curves known in hydrology to describe the series of river mineralization, using the example of the Dnipro basin, has also been analyzed. Using Pearson’s fitting criterion, the Pearson type III distributions and the three-parameter distributions by S.M.Krytsky and M.F.Menkel have been verified on their correspondence with the empirical series of mineralization. As a result, it was found that in 85% of cases the Pearson type III distribution can be used, and the three-parameter by S.M.Krytsky and M.F.Menkel can be used in 60% of cases.

INFLUENCE OF WATER MINERALIZATION ON ZOOPLANKTON PRODUCTIVITY IN RESERVOIRS OF AKMOLA REGION

The effect of water mineralization on zooplankton productivity in the reservoirs of the Akmola region is a very relevant study. The chemical composition of natural waters is inextricably linked to the composition and structure of the soil, which, in turn, was formed during the long evolution of the earth's crust under the influence of climate. Natural waters have a wide variety of qualitative and quantitative chemical composition. The basis for systematization in existing classifications are the amount of mineralization, the predominant component or group, the relationship between different values of concentrations of various ions, the presence of increased amounts of any specific elements of the gas and salt regimes. The relevance of the work is due to insufficient study of the fishing potential of medium-sized reservoirs in Northern Kazakhstan. The work aimed to study the mineralization of water as a factor determining the life of aquatic organisms. As a result of this research, data confirmed the position that the factors that determine the viability of marine organisms are the mineralization of water. To characterize zooplankton across the entire lake area, samples were taken, taking into account different stations. The number of individual organisms in the sample was determined. The degree of mineralization of lakes in the Akmola region was indicated. The correlation coefficient for features in the "mineralization – zooplankton abundance" pair was calculated as r = -0.96, and in the "mineralization – zooplankton biomass" pair r = -0.85. When analyzing the data obtained, it was observed an inverse relationship between water mineralization and zooplankton abundance. When water mineralization increases by 4.03% in July, the number of zooplankton organisms decreases from 170.03 to 152.6 thousand copies/m3. When the salinity of the Uyali - Shalkar lake water increases from 362 (May) to 508 mg/L (July), the zooplankton biomass decreases from 6.02 to 5.73 mg/m3.

A METHODOLOGICAL ASPECTS OF MONITORING SPRINGS IN THE CONDITIONS OF THE UKRAINIAN CARPATHIANS

The purpose of the study is to generalize methodological approaches to monitoring springs in the territory of the Carpathian National Natural Park and to identify the main hydrological and hydrochemical laws of their functioning. We conducted research of springs outlined outside the Carpathian National Natural Park (CNNP) – the first (1980) and one of the largest (504.95 km2) nature parks in Ukraine, located in the territory of Ivano-Frankivsk region. The office is located in Yaremche, Ivano-Frankivsk region. Inventory and accounting of springs is included in the plan of annual nature conservation activities held in the park. In 2019, after the grant of two wetlands in the Carpathian National Park (Prut and Pogorelets) international status and their inclusion in the list of wetlands protected by the Ramsar Convention on Wetlands of International Imports of International Imports especially as Waterfowl Habitat, 1971 – the study of environmental components, including springs, becomes more relevant and practical.There are all the necessary conditions for a comprehensive study of natural groundwater output to the surface – scientific, logistical and informational base. For the period 2011-2019 about 40 expeditions were conducted to study the springs located in three main natural complexes on the territory of the Carpathian NNP: the Outer Gorgany, the Yasinsky-Verkhovynsky intermountain basin, the Chernogora massif. In the hydrological aspect, it is a Prut river basin (left tributary of the Danube). The main methodological approaches for monitoring springs were developed and consistently implemented. The general monitoring algorithm, which is considered in the article, can be presented in the form of a flowchart with successive steps: 1) collecting available information about the springs 2) field surveys of the first level (description of the springs and their mapping) 3) field surveys of the second level (measuring flow rates and water sampling); 4) regular observations on reference springs. The water mineralization of most springs in the Carpathian NPP (about 90%) is in the range of “very fresh” and “normally fresh” – 30-500 mg/dm3 (according to the classification of Valentyn Khilchevskyi). There are springs with very low salt content – “extremely fresh” (10-30 mg/dm3), and sometimes “super fresh” with a salinity of less than 10 mg/dm3. Such a low level of spring water mineralization corresponds to the norms of the regional natural background of the salt content in unpolluted atmospheric precipitation.