Determination of Inorganic Ion in Biomaterials Using Capillary Ion Chromatography

Ion Chromatography is widely used analytical technique for determination of anionic and cationic analytes in various sample matrices. Columns of flexible fused-silica tubing packed with commercially available materials have been prepared. The microcolumns were prepared in the laboratory from fused silica tubing TSKgel IC-Anion-PWXL (100 mm long x 0,320 I.D). The parameters which affect the chromatographic retention behavior have been examined. For investigation of the optimum concentration of the eluent, the NaCl and KCl concentration was varied from 50 to 25 mM and operated at eluent flow rate 3 µL/min. The developed technique was successfully applied to the simultaneous determination of inorganic anions in real samples

Determination of Inorganic Ion in Biomaterials Using Capillary Ion Chromatography

Ion Chromatography is widely used analytical technique for determination of anionic and cationic analytes in various sample matrices. Columns of flexible fused-silica tubing packed with commercially available materials have been prepared. The microcolumns were prepared in the laboratory from fused silica tubing TSKgel IC-Anion-PWXL (100 mm long x 0,320 I.D). The parameters which affect the chromatographic retention behavior have been examined. For investigation of the optimum concentration of the eluent, the NaCl and KCl concentration was varied from 50 to 25 mM and operated at eluent flow rate 3 µL/min. The developed technique was successfully applied to the simultaneous determination of inorganic anions in real samples.