Determination of Inorganic Ion in Biomaterials Using Capillary Ion Chromatography

Ion Chromatography is widely used analytical technique for determination of anionic and cationic analytes in various sample matrices. Columns of flexible fused-silica tubing packed with commercially available materials have been prepared. The microcolumns were prepared in the laboratory from fused silica tubing TSKgel IC-Anion-PWXL (100 mm long x 0,320 I.D). The parameters which affect the chromatographic retention behavior have been examined. For investigation of the optimum concentration of the eluent, the NaCl and KCl concentration was varied from 50 to 25 mM and operated at eluent flow rate 3 µL/min. The developed technique was successfully applied to the simultaneous determination of inorganic anions in real samples

Determination of Inorganic Ion in Biomaterials Using Capillary Ion Chromatography

Ion Chromatography is widely used analytical technique for determination of anionic and cationic analytes in various sample matrices. Columns of flexible fused-silica tubing packed with commercially available materials have been prepared. The microcolumns were prepared in the laboratory from fused silica tubing TSKgel IC-Anion-PWXL (100 mm long x 0,320 I.D). The parameters which affect the chromatographic retention behavior have been examined. For investigation of the optimum concentration of the eluent, the NaCl and KCl concentration was varied from 50 to 25 mM and operated at eluent flow rate 3 µL/min. The developed technique was successfully applied to the simultaneous determination of inorganic anions in real samples.

Kadar Asam Klorogenat (CGA) Dalam Biji Kopi Arabika (Coffea Arabica) Asal Wamena, Papua

Chlorogenic acid (CGA) is one of chemical compound in coffee and class of polyphenolic compounds that have activity as antioxidant. This study aims to determine the CGA contain in Arabica coffee beans from Wamena regency of Jayawijaya, Papua. Coffee beans were roasted by three roasting temperature i.e. 75, 150 and 2250 C while unroasted one was used as controls. Extraction was performed by soxhletation using metanol as a solvent for 5 hours. Separation and CGAcontent were determined using HPLC uses C-18 column, 150 mm length, 4,6 mm in diameter, with metanol-fosfat buffer 10 mM pH 2,6(30:70) as eluent, flow rate of 1 mL/minutes, injection volume of 20 µL and measured by spectrophotometer UV-Vis in 329 nm. The result of the study shows the CGAcontent in treated beans with temperature roasting of 75, 150 and 2250C respectively were 6,93 ; 9,33 and 7,12 % while the unroasted one was 7,73 %. The lowest CGA content was found in coffee beans roasted in 750C while the highest in 1500 C.