Green Sample Preparation for Liquid Chromatography and Capillary Electrophoresis of Anionic and Cationic Analytes

2015 ◽  
Vol 87 (8) ◽  
pp. 4117-4123 ◽  
Author(s):  
Alain Wuethrich ◽  
Paul R. Haddad ◽  
Joselito P. Quirino
Keyword(s):  
Capillary Electrophoresis ◽  
Liquid Chromatography ◽  
Sample Preparation ◽  
Cationic Analytes ◽  
Green Sample

Study of the polymorphism of caprine milk caseins by capillary electrophoresis

1997 ◽  
Vol 64 (4) ◽  
pp. 515-523 ◽  
Author(s):  
ISIDRA RECIO ◽  
MARIA LUISA PÉREZ-RODRÍGUEZ ◽  
LOURDES AMIGO ◽  
MERCEDES RAMOS
Keyword(s):  
Capillary Electrophoresis ◽  
Liquid Chromatography ◽  
Sample Preparation ◽  
Cation Exchange ◽  
Genetic Polymorphisms ◽  
Quantitative Study ◽  
Milk Proteins ◽  
Capillary Electrophoresis Method ◽  
Caprine Milk ◽  
Electrophoresis Method

Polymorphism of caprine milk proteins was studied by capillary electrophoresis. Identification of casein (CN) fractions was effected by using isolated fractions from cation-exchange fast protein liquid chromatography. Genetic polymorphisms in caprine αs2-CN, αs1-CN, β-CN and κ-CN have been determined. κ-CN A and B, β-CN A and null, αs2-CN A, B and C, αs1-CN A, B, C and null, and other forms with intermediate and low αs1-CN content have been identified. The capillary electrophoresis method made it possible to analyse whole caprine milk using simple sample preparation and was rapid, automated and suitable for phenotyping studies. This method may also permit the quantitative study of different protein fractions.

Sulphonamide: Distribution, Toxicology, Environmental Characteristics, and Analysis - A Review

2020 ◽  
Vol 16 ◽  
Author(s):  
Kirubanandam Grace Pavithra ◽  
Vasudevan Jaikumar ◽  
Ponnusamy Senthil Kumar ◽  
PanneerSelvam SundarRajan
Keyword(s):  
Aqueous Solution ◽  
High Performance Liquid Chromatography ◽  
Capillary Electrophoresis ◽  
Liquid Chromatography ◽  
High Performance ◽  
Uv Spectroscopy ◽  
Relevant Information ◽  
Fluorometric Determination ◽  
Distinctive Features ◽  
The World

Background: Many antibiotics were widely used as medication based on their distinctive features. Among them, sulphonamides were commonly used, however their recalcitrant nature makes them difficult to dispose. Hence, their interaction with environment and analytic technique requires considerable attention globally. Objective: Therefore, this review aimed to provide detailed discussion about environmental as well as human health behaviour and analytic techniques corresponding to sulphonamides. Methods: Various results and discussion were extracted from technical journals and books published by different researchers from all over the world. The cited bibliographic references were intentionally investigated in order to extract relevant information related to proposed work. Results: In this review, the determination techniques such as UV-spectroscopy, Enthalpimetry, Immunosensor, Chromatography, Chemiluminescence, Photoinduced fluorometric determination, Capillary electrophoresis for sulphonamide determination were discussed in detail. Among them, High performance liquid chromatography (HPLC) and UV-spectroscopy was effective and extensively used for screening sulphonamide. Conclusion: Knowing the quantification and behaviour of sulphonamide in aqueous solution is mandatory to opt the suitable wastewater treatment required. Hence, choosing appropriate high precision and feasible screening techniques is necessary, which can be attained with this review.

Determination of Free Solanesol Levels in Cigarette Filters by Liquid Chromatography–Mass Spectrometry

2021 ◽  
Author(s):  
Roberto Bravo Cardenas ◽  
Phuong Ngac ◽  
Clifford Watson ◽  
Liza Valentin-Blasini
Keyword(s):  
Liquid Chromatography ◽  
Sample Preparation ◽  
Tobacco Smoke ◽  
Limit Of Detection ◽  
Internal Standard ◽  
Filter Material ◽  
Previous Method ◽  
Tobacco Smoke Exposure ◽  
Triple Quadrupole Mass Spectrometer ◽  
Cigarette Butts

Abstract Solanesol, a naturally occurring constituent of tobacco, has been utilized as a good marker for environmental tobacco smoke particulate and as a noninvasive predictor of mainstream cigarette smoke tar and nicotine intake under naturalistic smoking conditions. A fast and accurate method for measuring free solanesol to assess tobacco smoke exposure is highly desirable. We have developed and validated a new environmentally friendly, high-throughput method for measuring solanesol content in discarded cigarette filter butts. The solanesol deposited in the used filters can be correlated with mainstream smoke deliveries of nicotine and total particle matter to estimate constituent delivery to smokers. A portion of filter material is removed from cigarette butts after machine smoking, spiked with internal standard solution, extracted and quantitatively analyzed using reverse-phase liquid chromatography coupled to a triple-quadrupole mass spectrometer. The new method incorporates a 48-well plate format for automated sample preparation that reduces sample preparation time and solvent use and increases sample throughput 10-fold compared to our previous method. Accuracy and precision were evaluated by spiking known amounts of solanesol on both clean and smoked cigarette butts. Recoveries exceeded 93% at both low and high spiking levels. Linear solanesol calibration curves ranged from 1.9 to 367 µg/butt with a 0.05 µg/butt limit of detection.

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