Association of Polymorphism in Survivin gene and the risk of Liver Cancer resulting from Hepatitis C Virus among Egyptian patients

Background: This study aims to investigate the relation between Survivin gene polymorphisms, and the risk of Hepatocellular carcinoma (HCC) resulting from hepatitis C infection among Egyptian population. Methods: This prospective study was conducted on 164 patients, 57 patients were diagnosed with hepatitis C, where other 57 were diagnosed with HCC in addition to 50 healthy volunteers as controls. Genotyping for Survivin rs1042489 and rs8073069 single nucleotide polymorphisms was carried out by the allelic discrimination Real-Time Polymerase Chain Reaction Single Nucleotide Polymorphisms genotyping technology. Results: The results of Survivin rs1042489 polymorphism, revealed that the TC and CC genotypes were significantly different between hepatocellular carcinoma patients (OR=15.5, 95%CI: 3.299-72.825,P<0.001), and controls (OR=44, 95%CI: 8.025-241.254, P<0.001). Furthermore, CC genotype was significantly different between cirrhotic and hepatocellular carcinoma patients (OR=19.2, 95%CI: 3.097-119.049, P=0.002). Moreover the TC genotype shows a significant different between controls and cirrhotic patients (OR=5.5, 95%CI: 2.111-14.328, P<0.001). However when comparing TT genotypes, CC+TC genotypes results showed a significant association with increasing the risk of cirrhosis and hepatocellular carcinoma (OR=4.812, 95%CI: 1.893-12.233, P=0.001), (OR=21.607, 95%CI: 4.738-98.532, P<0.01), respectively. On the other hand, there was no significant difference among all studied groups for all genotypes, regarding Survivin rs8073069. Also CC+GC genotype showed no significant association with increased the risk of hepatocellular carcinoma (P=0.999) compared with the GG genotypes. Conclusion: The study indicates that functional Survivin rs1042489 polymorphism may contribute to the risk of hepatocellular carcinoma while Survivin rs8073069 polymorphism has no significant association with increased risk of hepatocellular carcinoma among the studied groups.

LncRNA AB209371 up-regulated Survivin gene by down-regulating miR-203 in ovarian carcinoma

AB209371 gene has been characterized as an oncogenic lncRNA in liver cancer. However, its involvement in ovarian carcinoma (OC) is unknown. In the present study, we analyzed the roles of AB209371 in OC. We found that AB209371 gene and Survivin gene were up-regulated in OC and positively correlated with OC development. AB209371 over-expression led to up-regulated Survivin in OC cells, while Survivin over-expression failed to affect AB209371. In addition, AB209371 over-expression led to down-regulated miR-203. However, miR-203 over-expression failed to affect AB209371, but down-regulated the expression of Survivin. In addition, over-expressions of AB209371 and Survivin resulted in the increased proliferation rate of OC cells. Over-expression MiR-203 played the opposite role and attenuated the effects of AB209371 over-expression. Therefore, AB209371 may down-regulate miR-203 to up-regulate Survivin, thereby promoting OC cell proliferation. Our study provided novel insights into the pathogenesis of OC.

The survivin gene expression in neoplastic hepatocytes from chickens infected with Marek’s virus

P53 protein is one of the main proteins in apoptosis pathway. The role of survivin protein in inhibition of P53 in different human cancers has been proved. The expression of survivin can be a main marker in diagnosis and prognosis of different human cancer. Until now, the survivin gene expression in birds infected with Marek’s disease was not investigated. In this study, liver tissue samples of chickens infected with Marek’s disease virus (MDV) was collected. The identification of MDV was carried out with PCR and histopathologic examination. After identification of MDV, the pathogenicity of infected virus was investigated with specific primers targeted on 132 bp tandem repeat. After this, the survivin gene expression was examined in neoplastic liver samples by Real-Time PCR. The PCR amplification of tumor liver samples showed that all samples were infected with MDV. The result of histopathology and amplification of 132 bp tandem repeat in tumor samples showed that the chickens were infected to pathogenic MDV. Results showed that the survivin gene expression in neoplastic hepatocytes was significantly higher than normal hepatocytes. In conclusion, survivin gene expression can be utilized as a suitable biomarker in diagnosis of Marek’s disease in birds. It seems that viral meq oncogene in Marek’s disease can play role in induction of survivin expression in this disease that it is necessary to be proved.