scholarly journals Probiotic Saccharomyces cerevisiae var. boulardii supernatant inhibits survivin gene expression and induces apoptosis in human gastric cancer cells

2020 ◽  
Author(s):  
Babak Pakbin ◽  
Shaghayegh Pishkhan Dibazar ◽  
Samaneh Allahyari ◽  
Maryam Javadi ◽  
Alireza Farasat ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Saccharomyces Cerevisiae ◽  
Cancer Cells ◽  
Human Gastric Cancer ◽  
Gastric Cancer Cells ◽  
Survivin Gene

POT1 deficiency alters telomere length and telomere-associated gene expression in human gastric cancer cells

2010 ◽  
Vol 19 (5) ◽  
pp. 345-351 ◽  
Author(s):  
Xiaoyan Ning ◽  
Shiming Yang ◽  
Rongquan Wang ◽  
Rugang Zhang ◽  
Liping Guo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Cancer Cells ◽  
Telomere Length ◽  
Human Gastric Cancer ◽  
Gastric Cancer Cells

Enhanced bax gene expression reduces cell growth and sensitizes human gastric cancer cells to chemotherapeutic agents

2000 ◽  
Vol 118 (4) ◽  
pp. A535
Author(s):  
Koga Komatsu ◽  
Jun-ichi Miyazaki ◽  
Susumu Suzuki ◽  
Michiro Otaka ◽  
Sumio Watanabe ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Cell Growth ◽  
Cancer Cells ◽  
Chemotherapeutic Agents ◽  
Human Gastric Cancer ◽  
Gastric Cancer Cells ◽  
Bax Gene

scholarly journals Activated Integrated Stress Response Induced by Salubrinal Promotes Cisplatin Resistance in Human Gastric Cancer Cells via Enhanced xCT Expression and Glutathione Biosynthesis

2018 ◽  
Vol 19 (11) ◽  
pp. 3389 ◽  
Author(s):  
Sheng-Fan Wang ◽  
Chih-Hsuan Wung ◽  
Meng-Shian Chen ◽  
Chian-Feng Chen ◽  
Pen-Hui Yin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Stress Response ◽  
Cancer Cells ◽  
Cisplatin Resistance ◽  
Translation Initiation Factor ◽  
Heme Oxygenase 1 ◽  
Human Gastric Cancer ◽  
Integrated Stress Response ◽  
Gastric Cancer Cells

The integrated stress response (ISR) pathway is essential for adaption of various stresses and is related to mitochondrion-to-nucleus communication. Mitochondrial dysfunction-induced reactive oxygen species (ROS) was demonstrated to activate general control nonderepressible 2 (GCN2)–eukaryotic translation initiation factor 2α (eIF2α)–activating transcription factor-4 (ATF4) pathway-mediated cisplatin resistance of human gastric cancer cells. However, whether or how ISR activation per se could enhance chemoresistance remains unclear. In this study, we used eIF2α phosphatase inhibitor salubrinal to activate the ISR pathway and found that salubrinal reduced susceptibility to cisplatin. Moreover, salubrinal up-regulated ATF4-modulated gene expression, and knockdown of ATF4 attenuated salubrinal-induced drug resistance, suggesting that ATF4-modulated genes contribute to the process. The ATF4-modulated genes, xCT (a cystine/glutamate anti-transporter), tribbles-related protein 3 (TRB3), heme oxygenase 1 (HO-1), and phosphoenolpyruvate carboxykinase 2 (PCK2), were associated with a poorer prognosis for gastric cancer patients. By silencing individual genes, we found that xCT, but not TRB3, HO-1, or PCK2, is responsible for salubrinal-induced cisplatin resistance. In addition, salubrinal increased intracellular glutathione (GSH) and decreased cisplatin-induced lipid peroxidation. Salubrinal-induced cisplatin resistance was attenuated by inhibition of xCT and GSH biosynthesis. In conclusion, our results suggest that ISR activation by salubrinal up-regulates ATF4-modulated gene expression, increases GSH synthesis, and decreases cisplatin-induced oxidative damage, which contribute to cisplatin resistance in gastric cancer cells.

scholarly journals The Antioxidant Alpha-Lipoic Acid Inhibits Proliferation and Invasion of Human Gastric Cancer Cells via Suppression of STAT3-Mediated MUC4 Gene Expression

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Yu Yang ◽  
Erhu Fang ◽  
Jiajun Luo ◽  
Hongxue Wu ◽  
Yue Jiang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Cancer Cells ◽  
Human Gastric Cancer ◽  
Alpha Lipoic Acid ◽  
Gastric Cancer Cells ◽  
Muc4 Expression ◽  
Cancer Tissues ◽  
Muc4 Gene ◽  
Proliferation And Invasion

Background. Metastasis and invasion are the main causes of mortality in gastric cancer. To improve the treatment of gastric cancer, the development of effective and innovative antitumor agents toward invasion and proliferation is needed. Alpha-lipoic acid (ALA), a naturally occurring thiol antioxidant, showed antiproliferative and cytotoxic effects on several cancers. So it is feasible to explore whether ALA can be used to inhibit proliferation and invasion in human gastric cancer. Methods. The expression of MUC4 in human gastric cancer tissues was assayed by immunohistochemistry. Then, we performed in vitro cell proliferation and invasion analysis to explore the antitumor effect of ALA using AGS, BGC-823, and MKN-28 cells. To further explore the mechanism of ALA-mediated downregulation of MUC4, we cotransfected human gastric cancer cells with STAT3 siRNA and STAT3 overexpression construct. ChIP assays were carried out to find the relationship between MUC4 and STAT3. Results. We found that the MUC4 gene was strongly expressed in human gastric cancer tissues. Meanwhile, ALA reduced proliferation and invasion of human gastric cancer cells by suppressing MUC4 expression. We also found that STAT3 was involved in the inhibition of MUC4 by ALA. Mechanistically, ALA suppressed MUC4 expression by inhibiting STAT3 binding to the MUC4 promoter region. Conclusion. ALA inhibits both proliferation and invasion of gastric cancer cells by suppression of STAT3-mediated MUC4 gene expression.

Quercetin induced cell apoptosis and altered gene expression in AGS human gastric cancer cells

2018 ◽  
Vol 33 (11) ◽  
pp. 1168-1181 ◽  
Author(s):  
Hung-Sheng Shang ◽  
Hsu-Feng Lu ◽  
Ching-Hsiao Lee ◽  
Han-Sun Chiang ◽  
Yung-Lin Chu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Cancer Cells ◽  
Cell Apoptosis ◽  
Human Gastric Cancer ◽  
Gastric Cancer Cells ◽  
Altered Gene Expression ◽  
Altered Gene
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