The Prevalence of Domesticated Geriatri Cats in Surabaya City at April-May 2019

This study is beneficial to give information about population of domesticated geriatric cats in Surabaya period from April to May 2019. This study is also beneficial to give information about likely risks of domesticated geriatric cats in Surabaya City based on gender, cat breed, and cat’s disease. The design of this study was Observational Retrospective using cross-sectional method. Sampling used stratified random sampling method. This study collected primary data and was analyzed descriptively using prevalence test and relative risks. That domesticated geriatric cats in Surabaya City period from April-May 2019, the oldest cats were written 17 years for male cats and 9 years for female cats. The prevalence of domesticated geriatric cats in Surabaya City period from April-May 2019 was 9 (7,5%). Geriatric cats distributed by the gender were 5 (4,2%) male and 4 (3,3%) female, where geriatric cats distributed by the cat breed were 5 (4,2%) pure breed, 4 (3,3%) mixed breed, and 0 (0%) domestic sample. The result of geriatric cats analysis based on gender showed that male cats had a change of 1,4 times higher compared to the female to reach geriatric, whereas based on cat breed it showed pure breed had a change 3,3 times compared to mixed breed to reach geriatric. On this study, the disease often appeared in domesticated geriatric cats in Surabaya City period from April -May 2019 were: on skin, respiration, digestion, and eyes. Based on relative risk analysis on geriatric cats, it had a change of gastrointetinal tract that was higher compared to other diseases.

Genomic prediction using a reference population of multiple pure breeds and admixed individuals

Background In dairy cattle populations in which crossbreeding has been used, animals show some level of diversity in their origins. In rotational crossbreeding, for instance, crossbred dams are mated with purebred sires from different pure breeds, and the genetic composition of crossbred animals is an admixture of the breeds included in the rotation. How to use the data of such individuals in genomic evaluations is still an open question. In this study, we aimed at providing methodologies for the use of data from crossbred individuals with an admixed genetic background together with data from multiple pure breeds, for the purpose of genomic evaluations for both purebred and crossbred animals. A three-breed rotational crossbreeding system was mimicked using simulations based on animals genotyped with the 50 K single nucleotide polymorphism (SNP) chip. Results For purebred populations, within-breed genomic predictions generally led to higher accuracies than those from multi-breed predictions using combined data of pure breeds. Adding admixed population’s (MIX) data to the combined pure breed data considering MIX as a different breed led to higher accuracies. When prediction models were able to account for breed origin of alleles, accuracies were generally higher than those from combining all available data, depending on the correlation of quantitative trait loci (QTL) effects between the breeds. Accuracies varied when using SNP effects from any of the pure breeds to predict the breeding values of MIX. Using those breed-specific SNP effects that were estimated separately in each pure breed, while accounting for breed origin of alleles for the selection candidates of MIX, generally improved the accuracies. Models that are able to accommodate MIX data with the breed origin of alleles approach generally led to higher accuracies than models without breed origin of alleles, depending on the correlation of QTL effects between the breeds. Conclusions Combining all available data, pure breeds’ and admixed population’s data, in a multi-breed reference population is beneficial for the estimation of breeding values for pure breeds with a small reference population. For MIX, such an approach can lead to higher accuracies than considering breed origin of alleles for the selection candidates, and using breed-specific SNP effects estimated separately in each pure breed. Including MIX data in the reference population of multiple breeds by considering the breed origin of alleles, accuracies can be further improved. Our findings are relevant for breeding programs in which crossbreeding is systematically applied, and also for populations that involve different subpopulations and between which exchange of genetic material is routine practice.

HIBRIDISASI INTERSPESIFIK TIGA SPESIES IKAN TOR (Tor soro, Tor douronensis, DAN Tor tambroides) SECARA RESIPROKAL PADA FASE LARVA

Ikan Tor merupakan genus yang potensi dibudidayakan karena memiliki nilai ekonomis tinggi. Masalah yang ada adalah lambatnya pertumbuhan dan rendahnya tingkat sintasan benih ikan tersebut dalam jumlah yang memadai untuk pembudidaya. Hibridisasi atau persilangan merupakan suatu upaya untuk mendapatkan kombinasi antara populasi yang berbeda untuk menghasilkan keturunan yang memiliki sifat unggul. Hibridisasi tiga spesies ikan Tor secara resiprokal antara Tor soro (Ts), Tor douronensis (Td), dan Tor tambroides (Tt) dilakukan guna untuk mengetahui keberhasilan hibridisasi beda spesies tetapi masih dalam genus. Metode yang digunakan pada penelitian ini adalah eksperimen dengan enam perlakuan dan tiga kali ulangan hasil hibrida. Hasil yang diperoleh menunjukkan nilai derajat pembuahan tertinggi dihasilkan dari hibridisasi Td >< Ts (93,66 ± 1,15%), daya tetas tertinggi dihasilkan dari persilangan Td >< Ts (88,00 ± 1,00%), laju pertumbuhan spesifik bobot tertinggi dihasilkan dari hibridisasi Ts >< Td dan Ts >< Tt (5,62 ± 0,17%; 5,44 ± 0,06%), laju pertumbuhan spesifik panjang tertinggi dihasilkan dari hibridisasi Ts >< Td (1,79 ± 0,11%) dan sintasan tertinggi dihasilkan dari hibridisasi Tt >< Ts (86,00 ± 3,60%). Nilai heterosisyang meliputi derajat pembuahan, daya tetas telur, pertumbuhan benih, dan sintasan ikan sampai umur 41 hari menunjukkan nilai negatif, kecuali laju pertumbuhan spesifik bobot. Hibridisasi berdampak pada meningkatnya jumlah larva abnormal yang dihasilkan dibanding tetuanya (pure breed).Tor fish has the potential to be commercially developed as a farmed fish due to its high economic value and market demand. However, slow growth and low survival rate of the fish larvae and insufficient seed availability for aquaculture farmers are the main bottlenecks to develop a profitable aquaculture for tor fish. Hybridization or crossbreed is an attempt to get a combination between different populations to produce offspring inheriting superior characters. A reciprocal hybridization between Tor soro (Ts), Tor douronensis (Td), and Tor tambroides (Tt) was carried out to assess the success rate of hybridization between the three species. The study used an experimental design with six treatments and three replications to measure and compare hybridization and survival parameters between the species. The highest fertilization rate attained by hybridization Td >< Ts (93.66 ± 1.15%, the highest hatching rate was achieved by Td >< Ts (88.00 ± 1.00%), the highest weight specific growth rate was gained by hybridization Ts >< Td and Ts >< Tt (5.62 ± 0.17%; 5.44 ± 0.06%), the highest lenght specific growth rate was achieved by hybridization Ts >< Td (1.79 ± 0.11%) and highest survival rate was resulted from hybridization between Tor tambroides >< Tor tambroides (90.33 ± 1.52%). The observed heterosis (degree of fertilization rate, hatching rate, growth rate, and survival rate until the age of 41 days) showed that all parameter characters were negative, except for the weight-specific growth rate characters. This study concludes that hybridization have an impact on increasing the number of abnormal larvae produced compared to their parents (pure-breed).

Study of the correlation between toxic, essential and macronutrients in the blood serum of stud horses

Studies were conducted to assess the elemental composition of the blood serum of 39 Arabian pure breed stud horses. The correlation between the content of toxic elements in the blood serum: aluminum (Al), arsenic (As), cadmium (Cd), lead (Pb), tin (Sn), mercury (Hg), essential trace elements: cobalt (Co), chromium (Cr), copper (Cu), iron (Fe), iodine (I), manganese (Mn), selenium (Se), zinc (Zn) and macronutrients: calcium (Ca), potassium (K), magnesium (Mg), phosphorus (P) was studied. A negative correlation was established between the content of mercury and iron in the blood serum of stud horses (r=-0.34; p=0.04). A negative correlation was also found between the levels of cadmium and calcium in the blood serum (r=-0.40; p=0.02). The establishment of these correlations suggests the presence of competitive relations between these elements in the body of horses. Positive correlations were established between the content of toxic elements in the blood serum: Al/As (r=0.38; p=0.02), Al/Pb (r=0.41; p=0.009), Al/Sn (r=0.56; p=0.002), As/Sn (r=0.46; p=0.0003), Pb/Sn (r=0.32; p=0.05).

The sperm longevity and freezability in the modified BHSV extender of Thai Pradu-hangdum chicken

Pradu-hangdum is a distinctive Thai native pure breed chicken. To conserve the pure breed of this chicken species, the artificial insemination was invested. The key to the highly successful output of this technique is high quality of semen. Hence, the objective of the present study is to characterize the Pradu-hangdam sperms and their longevity and freezability in the BHSV extender. The semen of the 30 randomized male chicken were collected. The macroscopic and microscopic examination were used to determine the sperm characters. The results revealed that the Pradu-hangdam sperms contained normal a spiral-shaped head, mid-piece and tail and normal white cream color. The mean number of sperm concentration was 5.24×109 ± 1.54 sperms/mL. The mean volume was 0.22 ± 0.08 mL. The results of longevity in mean total motility of sperm was 85.20%, 56.00% and 36.33% storage for 1, 24, and 48 hours after semen collection in extender, respectively. The longevity of sperm storage in extender decreased significantly at 24 and 48 hours (P<0.05). The freezability of Pradu-hangdam semen significant decreased from 81.45% to 57.02% of motility (P<0.05) but in the range of acceptable result for insemination. In conclusion, this study provides the basic knowledge of sperm characters and their longevity which decreases in relation to the time after collection even though it was preserved and frozen in the BHSV of the acceptable data. Furthermore, the freezing technique and fertility rate should be a further study in the long-term preservation of Pradu-hangdam sperms.

MEAT PRODUCTIVITY OF CROSSBREEDS WHEN CROSSING BESTUZHEV BREED WITH CHARILAIS BREED

The article presents the results of research on fattening and meat qualities of bestuzhev-charolaise crossbreeds (bulls) of various bloodlines. At the same time, bulls of the II generation (ӏӏӏ group) had the highest average daily growth (1085g), respectively, absolute (216kg) and relative growth (59.34%). In animals of the same group, less feed was spent on 1 kg of growth (6.3 feed units) and the best payment for feed was made with an increase (14.49 kg). All of these indicators of crossbred bulls of I and III generation (II and IV groups) also exceed bestuzhev purebred bulls, but inferior to the hybrids of II generation (III group). The results of the control slaughter, carried out at the age of 15 months, compared with the pure breed Bestuzhev cattle weight more carcasses crossbred bulls in all three groups have more carcasses, but the hybrid animals have the greatest III generation, then II generation ( III group) and I generation. Economic indicators of growing and fattening bestuzhev-charolaise bulls of different blood types showed that with an increase in blood content for the charolaise breed, the profit per 1 animal sold increases and the level of profitability of beef production increases in comparison with purebred Bestuzhev animals. Therefore, crossing a half-breed bestuzhevskih heifers with bulls Charolais to II and III generation economically feasible. Using this method of crossing will allow farms in the Middle Volga region to significantly increase the production of beef and improve its quality.

Image-Based Goat Breed Identification and Localization Using Deep Learning

In this paper an attempt has been made to identify six different goat breeds from pure breed goat images. The images of goat breeds have been captured from different organized registered goat farms in India, and almost two thousand digital images of individual goats were captured in restricted (to get similar image background) and unrestricted (natural) environments without imposing stress to animals. A pre-trained deep learning-based object detection model called Faster R-CNN has been fine-tuned by using transfer-learning on the acquired images for automatic classification and localization of goat breeds. This fine-tuned model is able to locate the goat (localize) and classify (identify) its breed in the image. The Pascal VOC object detection evaluation metrics have been used to evaluate this model. Finally, comparison has been made with prediction accuracies of different technologies used for different animal breed identification.

PRESERVATION OF GENETIC POTENTIAL OF STAVROPOL SHEEP BREED FOR POVOLZHYE DREY STEPPE ZONE

In conditions of Povolzhye steppe zone development of productive qualities of Stavropol breed sheep was conducted by meat merino sheep. Experimental research was carried out in ZAO «Novaya Zhizn» in Novouzensky district of Saratov region, located in semi desert zone, bordering upon South-West Khazakhstan. The research aim was the increase and maintenance of genetic potential of Stavropol breed sheep of Povolzhye population. Working procedure is based on guidelines of the All-Union Academy of Agricultural sciences, All Union research institute of sheep and goat breeding. The study materials were Stavropol pure breed sheep and 1/8 –thoroughbred cross breed with Australian meat merino herdmates. As the result of stepped breeding of local Stavropol ewe with half bred by Australian meat merino ram- breeder of Stavropol breed animals of 1/2 ,1/4, 1/8 –blood were obtained, which then one- shear tupped by breeding «inter se». It is proved that in applying such selective method 1/8-blood by Australian meat merino sheep were characterized with higher body weight, better wool quality in contrast to Stavropol pure –breed herdmates. So, in 1/8-blood by Australian meat merino of hybrid ewe during breeding «inter se» live body at birth was higher than at pure breed herdmates for 5,56 %, during ablactation –at the age of 4 months - for 6,38 % (difference isn’t accurate). According to sharing of wool in grease, superiority of ewe of hybrid origin over pure breed was 5,6 % (Р≥0,99), but in terms of washed fiber it increases to 8,3 % (Р≥0,999). So, in dry steppe zone of Povolzhye new improved, according to its productive qualities, genotype of Stavropol breed sheep is created with retention of genetic resource that is essential economic factor for the modern maintenance of fine-wool sheep breeding field.