Cryopreservation of semen of initial forms and chimeras of birds of two breeds: Sussex light and Poltava clay

Purpose: evaluation of the quality of seed chimer and source forms before and after cryopreservation.Materials and methods. For research, the breed of Sussex chickens and Poltava clay. Using these rocks, chimeras were obtained by the method of transplantation of blastodermal cells. An assessment of the quality of sperm of the roosters was carried out under a microscope at a temperature of 42 degrees. At various times, the activity of native sperm and its concentration was determined. Cryoconservation was carried out in small granules. The defrosting of the granules was produced on a heated metal plate at a temperature of 60 ° C.Results. A comparative analysis of the volume of the ejaculate showed that Himer on average it was somewhat larger than that of the initial forms. The activity of sperms in native genital cells was sufficiently high in all studied samples, and was 80% or more. The concentration of sperm retained the same trend and practically did not differ from each other in all studied samples. The activity of frozen-fatty seed of the initial breeds and their chimer was significantly different. The maximum activity was observed in the Sussex breed and reliably below (p≤ 0.01) it has the remaining experimental groups. The activity of frozen-fatty seed turned out to be equal in chimeric organisms and Poltava clay, although Himer recipients were sussexes. It should be noted that the swing of oscillations according to the degree of activity of frozen-fatty seed in chimeric birds was significant (from 10 to 40 points), and the number of observations is small.Conclusion. The study assess the quality of seed chimer and the source forms before and after cryopreservation. It was demonstrated that after defrosting the activity of frozen-fatty seed of the initial rocks and their chimer was different.

Vitrification of early avian blastodermal cells with a new type of cryocontainer

Although cryopreservation of avian semen is only applicable for singlegene traits, cryopreservation of avian blastodermal cells could facilitate preservation of the entire genome of endangered or rare-breed poultry. Slow freezing methods result in acceptable survival rates; however, there are apparently no reports regarding the use of vitrification. The aim of the study was to establish methods for chicken embryonic cell vitrification, including development of a container which supported cryopreservation of large numbers of cells (to increase the probability of chimera production). Based on a preliminary study, vitrification seemed to be practical for avian blastodermal cell preservation. Pieces of mosquito net as carrier increased live cell rates compared to pellet form in media containing two macromolecules. Furthermore, we concluded that fetal calf serum in the vitrification medium could be replaced by polyvinylpyrrolidone, a chemically defined substance free of unwanted growth factors and potential pathogens.