The Hepatoprotective Effect of Acalypha wilkesiana Muell Arg. Leaves on some Biochemical Parameters in Wistar Albino Rats

2015 ◽  
Vol 4 (7) ◽  
pp. 652-661
Author(s):  
O. Sule ◽  
A. Abdu ◽  
I. Elekwa
Keyword(s):  
Biochemical Parameters ◽  
Hepatoprotective Effect ◽  
Albino Rats ◽  
Acalypha Wilkesiana ◽  
Wistar Albino Rats

scholarly journals THERAPEUTIC EFFECT OF NYMPHAEA ALBA LINN. FLOWERS AGAINST ISONIAZID-INDUCED HEPATOTOXICITY: AN EXPERIMENTAL STUDY

2018 ◽  
Vol 11 (5) ◽  
pp. 333
Author(s):  
Mohammad Nasiruddin ◽  
Irfan Ahmad Khan ◽  
Sayeedul Hasan Arif
Keyword(s):  
Biochemical Parameters ◽  
Ethanolic Extract ◽  
Antioxidant Property ◽  
Albino Rats ◽  
Marker Enzymes ◽  
Liver Marker Enzymes ◽  
Stress Markers ◽  
Significant Reversal ◽  
Nymphaea Alba ◽  
Wistar Albino Rats

Objective: This study was designed to evaluate the effect of ethanolic extract of Nymphaea alba (NAEE) Linn. flowers on liver marker enzymes, histology, and antioxidant tests against isoniazid (INH)-induced hepatotoxicity in rats.Methods: Wistar albino rats were treated with INH (50 mg/kg) for 28 days to induce hepatotoxicity. Silymarin (100 mg/kg) and NAEE Linn flowers in 200 mg/kg and 400 mg/kg doses, respectively, were used as standard and test drugs. Liver marker enzymes and histological examination of livers were performed to demonstrate the effect of NAEE against INH-induced hepatotoxicity. Catalase [CAT], glutathione, and malondialdehyde (MDA) levels were estimated to evaluate the antioxidant property of the NAEE extract.Results: The NAEE extract in 200 mg/kg and 400 mg/kg doses significantly decreased INH-induced elevation of liver marker enzymes as well as oxidative stress markers (CAT, glutathione, and MDA) in rats. Changes in biochemical parameters were supported by histological improvements of the liver.Conclusion: The NAEE Linn flowers in 200 mg/kg and 400 mg/kg doses showed a significant reversal of hepatic damage which was induced by INH in rats.

scholarly journals Biochemical Parameters, Activity Levels of Marker Enzymes and Serum Electolytes of Wistar Albino Rats Fed Mango (Mangifera indica) Pulp Formulated Diets

2019 ◽  
pp. 1-10
Author(s):  
Pauline N. Iheagwam ◽  
Eugene N. Onyeike ◽  
Benjamin A. Amadi
Keyword(s):  
Biochemical Parameters ◽  
Calcium Carbide ◽  
Albino Rats ◽  
Activity Levels ◽  
Marker Enzymes ◽  
Serum Electrolytes ◽  
Formulated Diets ◽  
Significant Difference ◽  
Wistar Albino Rats ◽  
Unripe Fruits

Biochemical parameters, activity levels of marker enzymes and serum electrolytes of Wistar albino rats fed naturally ripe (control), unripe and artificially ripened mango pulp formulated diets were investigated. Ripe and unripe fruits were collected and used for the study and artificially ripened mangoes were obtained by wrapping unripe fruits with dark polyethylene bag; treatment with calcium carbide and by dipping into hot water. After ripening, the mango samples were air-dried, pulverized and used to formulate the 10%, 20%, 30% diets fed to rats for 28 days and their plasma collected for biochemical investigations. At 10% level of incorporation, plasma protein concentration in the control (65.63±0.12 mg/dl) was significantly higher (p< 0.05) than values for unripe groups (55.50±1.91 mg/dl) and calcium carbide (56.97±1.27 mg/dl) while Albumin concentration was highest in the unripe (41.33±3.58 mg/dl) but lowest in the calcium carbide (33.50±0.69 mg/dl) but at 20 and 30% levels, no significant difference was observed for both parameters. Billirubin was significantly higher in the calcium carbide groups but lowest in the control groups at all levels of incorporation while Creatinine and Urea revealed no significant difference at all levels. Marker enzyme assay showed significant variations only in the concentrations of Alkaline phosphatase and Gamma glutamyl transterase at the 30% level of incorporation only. Electrolyte assay revealed that at 30% level of incorporation, significantly lower levels of potassium were observed in the calcium carbide (3.43±0.06 mg/dl) group compared to the control (3.80±0.17 mg/dl).Thus, artificial ripening of fruits may adversely affect Biochemical parameters, activity levels of marker enzymes and serum electrolytes.

scholarly journals Copper-induced changes of lipid peroxidation and hemato-biochemical parameters in rat blood: Protective role of flavonoids

2014 ◽  
Vol 66 (3) ◽  
pp. 1271-1279 ◽  
Author(s):  
Jelena Mladenovic ◽  
Milica Paunovic ◽  
Milos Matic ◽  
Veroljub Knezevic ◽  
Branka Ognjanovic ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Lactate Dehydrogenase ◽  
Biochemical Parameters ◽  
Lipid Peroxides ◽  
Protective Role ◽  
Albino Rats ◽  
Biochemical Processes ◽  
Wistar Albino Rats ◽  
Induced Changes

The effects of subchronic exposure to copper (Cu) on lipid peroxidation, hemato-biochemical parameters, and the possible protective role of flavonoids Quercetin and (-)-Epicatechin were studied. Male Wistar albino rats were treated with Cu (560 mg/L, p.o. as CuCl2?2H2O for 5 weeks) and Quercetin and (-)-Epicatechin (40 mg/kg BW each, i.p., every third day during the last 3 weeks) alone or in combination. Cu increased the concentration of lipid peroxides, decreased the number of erythrocytes, hemoglobin and hematocrit values and increased the activities of aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase. Coadministration of Quercetin and (-)-Epicatechin with Cu lowered the process of lipid peroxidation and restored examined hemato-biochemical parameters to control values. Our results indicate that Cu induced oxidative damage in erythrocytes, which led to anemia, while Quercetin and (-)-Epicatechin showed a protective effect on the hemato-biochemical processes in the blood of rats.

scholarly journals The Effects of Subchronic Methionine Overload Administered Alone or Simultaneously with L-cysteine or N-acetyl-L-cysteine on Body Weight, Homocysteine Levels and Biochemical Parameters in the Blood of Male Wistar Rats

2016 ◽  
Vol 17 (3) ◽  
pp. 215-224
Author(s):  
Zarko Micovic ◽  
Aleksandra Stamenkovic ◽  
Tamara Nikolic ◽  
Marija Stojanovic ◽  
Ljiljana Scepanovic ◽  
...  
Keyword(s):  
Body Weight ◽  
Biochemical Parameters ◽  
Genetic Disorders ◽  
The Body ◽  
Control Group ◽  
Albino Rats ◽  
Rat Serum ◽  
Male Wistar Rats ◽  
Total Homocysteine ◽  
Wistar Albino Rats

AbstractHyperhomocysteinemia (HHC), both basal and after methionine load, may occur due to genetic disorders or deficiencies of nutrients that affect the remethylation or trans-sulphuration pathways during methionine metabolism. HHC is involved in the pathogenesis of many illnesses as a result of its prooxidative effect and its impairment of antioxidative protection. The aim was to examine the effects of subchronic methionine overload on the body weight and standard biochemical parameters in rat serum and to examine whether simultaneous subchronic intraperotoneal administration of methionine alone or together with L-cysteine or N-acetyl-cysteine resulted in a change in the body weight and biochemical parameters in the rat serum. The research was conducted during a three-week period (male Wistar albino rats, n=36, body weight of approximately 160 g, age of 15-20 days), and the animals were divided into a control group and three experimental groups of 8-10 animals each: a) control group (0.9% sodium chloride 0.1-0.2 ml/day); b) methionine (0.8 mmol/kg/bw/day) (MET group); c) methionine (0.8 mmol/kg/bw/day) + L-cysteine (7 mg/kg/bw/day) (L-cys+MET group); and d) methionine (0.8 mmol/kg/bw/day) + N-acetyl-L-cysteine (50 mg/kg/bw/day) (NAC+MET group). In addition to the body weight monitoring, the levels of total homocysteine and the standard biochemical parameters in blood samples (plasma or serum) were determined. The results indicated that monitoring the homocysteine levels and standard biochemical parameters in blood could be used for analysis and could provide an excellent guideline for distinguishing between toxic and non-toxic doses of methionine intake, which may be meaningful for clinical applications.

scholarly journals The Larval Stage of Taenia taeniaeformis in Wistar Albino Rats with an Illustration of Biochemical and Histopathological Changes

2021 ◽  
Author(s):  
S. Shivaraju ◽  
Swapan Kumar Maiti ◽  
Divya Mohan ◽  
E. Kalaiselvan ◽  
S.P. Sunil Kumar ◽  
...  
Keyword(s):  
Experimental Study ◽  
Biochemical Parameters ◽  
Histopathological Examination ◽  
Diagnostic Tools ◽  
Albino Rats ◽  
Gamma Glutamyl Transferase ◽  
Morphological Examination ◽  
Abdominal Exploration ◽  
Wistar Albino Rats ◽  
Glutamyl Transferase

Background: Performing all the diagnostic tools before subjecting animals for designed experimental study is time consuming and economically not feasible. Furthermore, it may divert the researchers from original planned work to other areas. But these factors not acceptable to compromise in the assessment of effect of different regenerative agents, drugs and herbal preparation on liver. This is important to prevent any diversions in the actual results of the research due to the prior infection caused by Strobilocercus fasciolaris the lab animals. The study aimed to detect Strobilocercus fasciolaris infected Wistar albino rats using biochemical parameters as a diagnostic marker before subjecting the animals to any designed experimental study. Methods: The study mainly utilizes abdominal exploration, morphological examination of the larvae, histopathological examination of liver and finally the biochemical parameters changes in the concerned animal. 192 animals used for this study in that 32 had single to multiple cysts in the liver on abdominal exploration. Characteristic Strobilocercus larval morphology, histopathological alterations on liver and serological biomarkers was studied. Result: Our results showed that the serum levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, glucose, gamma glutamyl transferase, blood urea nitrogen, and total protein can be used as indicators to measure the severity of Strobilocercus fasciolaris larva infection in rats.

scholarly journals Acute and Sub chronic Toxicological evaluation of ethanolic leaf extract of Sida acuta Burm.F in Wistar albino rats by analyzing biochemical, heamatological and histochemical parameters

2020 ◽  
Vol 11 (3) ◽  
pp. 4111-4119
Author(s):  
Perumalsamy Muneeswari ◽  
Sri Rashmy Madathi ◽  
Kannappan Poornima
Keyword(s):  
Body Weight ◽  
Experimental Analysis ◽  
Biochemical Parameters ◽  
Histopathological Examination ◽  
Ethanolic Extract ◽  
Albino Rats ◽  
Toxicity Evaluation ◽  
Subchronic Toxicity ◽  
Sida Acuta ◽  
Wistar Albino Rats

Sida acuta burm.f belongs to Malvaceae, the mallow family and enjoys tropical and pantropical distribution. The plant is usually known as wireweed in the countryside, and it is highly medicinally valued traditionally and ethnobotanically promised. The species have literature reports on scientific attributes like abortifacient, anthelmintic, diuretic, anti-rheumatic, antipyretic and wound healing properties. The present study is concerned with acute and sub-chronic toxicity evaluation of ethanolic extract of Sida acuta Burm.f leaves in Wistar albino rats. Acute toxicity evaluation was conducted for 14 days. Acute doses of 100, 250, 500, 1000 and 2000 mg/kg BW were administrated to test groups of animals under consideration on the first day of experimental evaluation with three animals in each of total six groups along with control. For the remaining 13 days, animals were observed for noted behavioural changes and body weight were recorded respectively for 7th and 14th day of experimental analysis. At the end of the trial period, all the animals were euthanised, and various biochemical parameters and histopathological examination were carried out to assess the toxicity of extract. The present study revealed that the ethanolic extract of Sida acuta Burm.f leaves is non-toxic up to 2000mg/kg body weight. Subchronic toxicity evaluation was conducted for 28 days with several doses 100, 200, 300, 400 and 500mg/kg BW. Control rats without any treatment were maintained during the entire period of experimental analysis. The results of subchronic toxicity parameters indicate no significant changes to the biochemical parameters (glucose, urea, uric acid, creatinine, AST, ALT and Cholesterol) haematological and histopathological observation in comparison to the control groups. Based on subchronic toxicity parameters data, effective doses (200 and 400mg/kg BW) is determined for further cancer (colon) study in Wistar albino rats.

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