scholarly journals Effects of n-butanol fraction of Gongronema latifolium leave extract on some biochemical parameters in CCl4- induced oxidative damage in Wistar albino rats

2014 ◽  
Vol 8 (2) ◽  
pp. 52-64 ◽  
Author(s):  
J. C Okpala, ◽  
I Sani, ◽  
R Abdullahi, ◽  
H. N Ifedilichukwu, ◽  
J. C Igwe,
Keyword(s):  
Oxidative Damage ◽  
Biochemical Parameters ◽  
Albino Rats ◽  
Butanol Fraction ◽  
Wistar Albino Rats ◽  
Gongronema Latifolium

scholarly journals Effects of n-butanol fraction of Gongronema latifolium leave extract on some kidney function and histological parameters in CCl4-induced oxidative damage in Wistar albino rats.

2012 ◽  
Vol 2 (2) ◽  
pp. 119-131
Author(s):  
Okpala J.C. ◽  
Ifedilichukwu H.N.
Keyword(s):  
Oxidative Damage ◽  
Olive Oil ◽  
Kidney Function ◽  
Normal Control ◽  
Design Method ◽  
Control Group ◽  
Albino Rats ◽  
Butanol Fraction ◽  
Wistar Albino Rats ◽  
Gongronema Latifolium

Effects of n-butanol fraction of Gongronema latifolium leave extract on some kidney function and histological parameters in (CCl4) carbon tetrachloride-induced oxidative damage in Wistar albino rats were assessed. Fifty-four (54) Wistar albino rats were divided into treatment and LD50 groups. The treatment group was further divided into seven groups of 6 animals each by the randomized random design method, each were allowed food and water ad libidium. Group A (normal control) was given feed and water, Group B (vehicle control) was injected with olive oil intraperitoneally, while the rest groups (C, D, E, F and G) were injected intraperitoneally with a single dose of CCl4 (148 mg/kg) at 1:1 (v/v) solution in olive oil and all the animals were fasted for 36 hours. This was repeated once every week for a period of four (4) weeks. At the end of 28 days of treatment, there was significant (p<0.05) reduction in weight change of CCl4-induced control rats when compared with the normal control and induced treated groups. Kidney function studies showed that there was significant (p<0.05) increase in creatinine and urea levels of CCl4-induced control group when compared with the normal control and induced treated groups but there was no significant (p>0.05) difference between the normal control and induced treated groups. Also, the kidney homogenate revealed significant (p<0.05) decrease in superoxide dismutase, catalase and glutathione peroxidase activities in CCl4-induced control rats when compared with the normal control rats but there was no significant (p>0.05) difference between the normal control and induced treated groups. However, malondialdehyde concentration was significantly (p<0.05) higher in CCl4-induced control rats when compared with the normal control and induced treated rats. These findings suggested that n-butanol fraction of methanolic leave extract of G. latifolium may have anti-nephrotoxic and antioxidative effects against CCl4-induced kidney damage in rats.

The protective role of ferulic acid on sepsis-induced oxidative damage in Wistar albino rats

2014 ◽  
Vol 38 (3) ◽  
pp. 774-782 ◽  
Author(s):  
Merve Bacanlı ◽  
Sevtap Aydın ◽  
Gökçe Taner ◽  
Hatice Gül Göktaş ◽  
Tolga Şahin ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Ferulic Acid ◽  
Protective Role ◽  
Albino Rats ◽  
Wistar Albino Rats

Does rosmarinic acid treatment have protective role against sepsis-induced oxidative damage in Wistar Albino rats?

2016 ◽  
Vol 35 (8) ◽  
pp. 877-886 ◽  
Author(s):  
M Bacanlı ◽  
S Aydın ◽  
G Taner ◽  
HG Göktaş ◽  
T Şahin ◽  
...  
Keyword(s):  
Dna Damage ◽  
Oxidative Damage ◽  
Rosmarinic Acid ◽  
Biological Activities ◽  
Albino Rats ◽  
Repair Capacity ◽  
Tnf Α ◽  
Liver And Kidney ◽  
Wistar Albino Rats ◽  
Α Level

Reactive oxygen species are believed to be involved in the development of sepsis. Plant-derived phenolic compounds are thought to be possible therapeutic agents against sepsis because of their antioxidant properties. Rosmarinic acid (RA) is a phenolic compound commonly found in various plants, which has many biological activities including antioxidant activity. The aim of this study was to investigate the effects of RA on sepsis-induced DNA damage in the lymphocytes and liver and kidney cells of Wistar albino rats by alkaline comet assay with and without formamidopyrimidine DNA glycosylase protein. The oxidative stress parameters such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and total glutathione (GSH) and malondialdehyde (MDA) levels in the liver and kidney tissues and an inflammatory cytokine, tumor necrosis factor α (TNF-α) level in plasma were also evaluated. It is found that DNA damage in the lymphocytes, livers, and kidneys of the RA-treated rats was significantly lower than that in the sepsis-induced rats. RA treatment also decreased the MDA levels and increased the GSH levels and SOD and GSH-Px activities in the livers and kidneys of the sepsis-induced rats. Plasma TNF-α level was found to be decreased in the RA-treated rats. It seems that RA might have a role in the attenuation of sepsis-induced oxidative damage not only by decreasing the DNA damage but also by increasing the antioxidant status and DNA repair capacity of the animals.

scholarly journals N-Acetylcysteine Protects against the Anxiogenic Response to Cisplatin in Rats

Biomolecules ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 892 ◽  
Author(s):  
Rade Vukovic ◽  
Igor Kumburovic ◽  
Jovana Joksimovic Jovic ◽  
Nemanja Jovicic ◽  
Jelena S. Katanic Stankovic ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Elevated Plus Maze ◽  
Anxiolytic Effect ◽  
Control Group ◽  
Albino Rats ◽  
Behavioral Testing ◽  
Anxiogenic Effect ◽  
Plus Maze ◽  
Wistar Albino Rats ◽  
Cisplatin Therapy

Since cisplatin therapy is usually accompanied with numerous toxicities, including neurotoxicity, that involve tissue oxidative damage, the aim of this study was to evaluate the possible protective effect of N-acetylcysteine (NAC) on the anxiogenic response to cisplatin (CIS). Thirty-two male Wistar albino rats divided into four groups (control, cisplatin, NAC, and CIS + NAC). All treatments were delivered intraperitoneally. On day one, the control and cisplatin groups received saline while the NAC and CIS + NAC groups were administered with NAC (500 mg/kg). On the fifth day, the control group received saline while the CIS group was treated with cisplatin (7.5 mg/kg), the NAC group again received NAC (500 mg/kg), and the CIS + NAC group was simultaneously treated with cisplatin and NAC (7.5 and 500 mg/kg, respectively). Behavioral testing, performed on the tenth day in the open field (OF) and elevated plus maze (EPM) tests, revealed the anxiogenic effect of cisplatin that was significantly attenuated by NAC. The hippocampal sections evaluation showed increased oxidative stress (increased lipid peroxidation and decline in antioxidant enzymes activity) and proapoptotic action (predominantly by diminished antiapoptotic gene expression) following a single dose of cisplatin. NAC supplementation along with cisplatin administration reversed the prooxidative and proapoptotic effects of cisplatin. In conclusion, the results obtained in this study confirmed that antioxidant supplementation with NAC may attenuate the cisplatin-induced anxiety. The mechanism of anxiolytic effect achieved by NAC may include the decline in oxidative damage that down regulates increased apoptosis and reverses the anxiogenic action of cisplatin.

scholarly journals THERAPEUTIC EFFECT OF NYMPHAEA ALBA LINN. FLOWERS AGAINST ISONIAZID-INDUCED HEPATOTOXICITY: AN EXPERIMENTAL STUDY

2018 ◽  
Vol 11 (5) ◽  
pp. 333
Author(s):  
Mohammad Nasiruddin ◽  
Irfan Ahmad Khan ◽  
Sayeedul Hasan Arif
Keyword(s):  
Biochemical Parameters ◽  
Ethanolic Extract ◽  
Antioxidant Property ◽  
Albino Rats ◽  
Marker Enzymes ◽  
Liver Marker Enzymes ◽  
Stress Markers ◽  
Significant Reversal ◽  
Nymphaea Alba ◽  
Wistar Albino Rats

Objective: This study was designed to evaluate the effect of ethanolic extract of Nymphaea alba (NAEE) Linn. flowers on liver marker enzymes, histology, and antioxidant tests against isoniazid (INH)-induced hepatotoxicity in rats.Methods: Wistar albino rats were treated with INH (50 mg/kg) for 28 days to induce hepatotoxicity. Silymarin (100 mg/kg) and NAEE Linn flowers in 200 mg/kg and 400 mg/kg doses, respectively, were used as standard and test drugs. Liver marker enzymes and histological examination of livers were performed to demonstrate the effect of NAEE against INH-induced hepatotoxicity. Catalase [CAT], glutathione, and malondialdehyde (MDA) levels were estimated to evaluate the antioxidant property of the NAEE extract.Results: The NAEE extract in 200 mg/kg and 400 mg/kg doses significantly decreased INH-induced elevation of liver marker enzymes as well as oxidative stress markers (CAT, glutathione, and MDA) in rats. Changes in biochemical parameters were supported by histological improvements of the liver.Conclusion: The NAEE Linn flowers in 200 mg/kg and 400 mg/kg doses showed a significant reversal of hepatic damage which was induced by INH in rats.

scholarly journals Biochemical Parameters, Activity Levels of Marker Enzymes and Serum Electolytes of Wistar Albino Rats Fed Mango (Mangifera indica) Pulp Formulated Diets

2019 ◽  
pp. 1-10
Author(s):  
Pauline N. Iheagwam ◽  
Eugene N. Onyeike ◽  
Benjamin A. Amadi
Keyword(s):  
Biochemical Parameters ◽  
Calcium Carbide ◽  
Albino Rats ◽  
Activity Levels ◽  
Marker Enzymes ◽  
Serum Electrolytes ◽  
Formulated Diets ◽  
Significant Difference ◽  
Wistar Albino Rats ◽  
Unripe Fruits

Biochemical parameters, activity levels of marker enzymes and serum electrolytes of Wistar albino rats fed naturally ripe (control), unripe and artificially ripened mango pulp formulated diets were investigated. Ripe and unripe fruits were collected and used for the study and artificially ripened mangoes were obtained by wrapping unripe fruits with dark polyethylene bag; treatment with calcium carbide and by dipping into hot water. After ripening, the mango samples were air-dried, pulverized and used to formulate the 10%, 20%, 30% diets fed to rats for 28 days and their plasma collected for biochemical investigations. At 10% level of incorporation, plasma protein concentration in the control (65.63±0.12 mg/dl) was significantly higher (p< 0.05) than values for unripe groups (55.50±1.91 mg/dl) and calcium carbide (56.97±1.27 mg/dl) while Albumin concentration was highest in the unripe (41.33±3.58 mg/dl) but lowest in the calcium carbide (33.50±0.69 mg/dl) but at 20 and 30% levels, no significant difference was observed for both parameters. Billirubin was significantly higher in the calcium carbide groups but lowest in the control groups at all levels of incorporation while Creatinine and Urea revealed no significant difference at all levels. Marker enzyme assay showed significant variations only in the concentrations of Alkaline phosphatase and Gamma glutamyl transterase at the 30% level of incorporation only. Electrolyte assay revealed that at 30% level of incorporation, significantly lower levels of potassium were observed in the calcium carbide (3.43±0.06 mg/dl) group compared to the control (3.80±0.17 mg/dl).Thus, artificial ripening of fruits may adversely affect Biochemical parameters, activity levels of marker enzymes and serum electrolytes.

scholarly journals Determination of Some Biochemical Parameters in Streptozotocin-Induced Diabetic Albino Rats Pre-Treated and Post-Treated with Vernonia amygdalina and Gongronema latifolium Extracts

2019 ◽  
pp. 1-12
Author(s):  
Ibifiri Bristol ◽  
Ebirien-Agana Samuel Bartimaeus ◽  
Holy Brown
Keyword(s):  
Diabetes Mellitus ◽  
Biochemical Parameters ◽  
Density Lipoprotein ◽  
Treatment Phase ◽  
Lipoprotein Cholesterol ◽  
Atherogenic Index ◽  
Albino Rats ◽  
Vernonia Amygdalina ◽  
Atherogenic Index Of Plasma ◽  
Gongronema Latifolium

The use of herbs in the management of diabetes mellitus and its complications have been reported. This study was thus aimed at determining the levels of some biochemical parameters in streptozotocin-induced diabetic albino rats pre-treated and post-treated with Vernonia amygdalina and Gongronema latifolium extracts. Fifty (50) albino rats weighing between 150 – 250 g were used for this study. 25 albino rats were used for each phase of the treatment. The pre-treatment phase involved the treatment of the rats with 400 mg/kg b.w (singly) and 200 mg/kg b.w (combined) extracts for 14 days, after which diabetes mellitus was induced using streptozotocin before the rats were sacrificed. The post-treatment phase involved the inducement of diabetes with streptozotocin after which the rats were treated with 400 mg/kg b.w (singly) and 200 mg/kg b.w (combined) extracts for 28 days before the animals were sacrificed. Blood was collected via cardiac puncture and plasma/serum was collected. Total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), and total antioxidant capacity (TAC) were determined using standard procedures while glucose was measured with a glucometer. Cardiovascular risk ratios such as Castelli risk ratio I and II and atherogenic index of plasma were also calculated. Results showed that extracts of V. amygdalina (only), G. latifolium (only) and V.A+ GL (combined)significantly (p<0.05) reduced the TC, LDL-C , FBS, MDA and TAC levels while HDL-C level significantly (p<0.05) increased. The cardiac risk indices (CRI-I and CRI-II, and Atherogenic index of plasma (AIP) in both the pre-treated and post-treated rats were also reduced when compared to diabetic control. It is thus evident that these plants’ extracts possess hypoglycaemic, hypolipidaemic, and antioxidant properties and thus could be used to reduce cardiovascular risks. Therefore, the tradomedicinal use of these plants in the management of cardiovascular complications is highly recommended.

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