Ethanol Production from the Biomass of Two Marine Algae, Padina tetrastromatica and Sargassum vulgare

2016 ◽  
Author(s):  
Sanjeeva Rao Durbha ◽  
Surya Santosh Mohan Dev Tavva ◽  
Girijashankar Guntuku ◽  
Prabhakar Tadimalla ◽  
Venkateswara Rao Yechuri ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Marine Algae ◽  
Padina Tetrastromatica ◽  
Sargassum Vulgare

Anticoagulant activity of sulfated polysaccharides and polyphenols extracted from marine algae

2020 ◽  
Vol 16 ◽  
Author(s):  
Saidani Karima ◽  
Ziani Nadia ◽  
Touati Naima ◽  
Merzouk Hafida ◽  
Bedjou Fatiha
Keyword(s):  
Marine Algae ◽  
Anticoagulant Activity ◽  
Sugar Content ◽  
Ethanol Extract ◽  
Acetone Extract ◽  
Sulfated Polysaccharides ◽  
Brown Seaweeds ◽  
Sargassum Vulgare ◽  
Rhodomela Confervoides

: The aim of this study was to evaluate the anticoagulant activity of five marine algae from Bejaia’s coast (Algeria). Higher contents of phenolic compounds were obtained with ethanol for Cystoseira humilis, Halopteris scoparia, Padina pavonica and Rhodomela confervoides (8.55±0.29, 7.84±0.47, 6.41±0.14 and 4.16±0.04 mg CE/g of dw, respectively). Whereas, for Sargassum vulgare, the extraction with acetone showed higher content (3.04±0.071 mg CE/g of dw).The determination of sugar content showed that acetone extract of the five seaweeds was the richest in sugar, this rate increasingly dropped in ethanol extract and the fractions A and B. The red algae Rhodomela confervoides showed a significant anticoagulant activity in ethanol extract and the fractions A, B and C, with an elongation up to 407.97±58.12 s in the fraction C, at a concentration of 10 mg/mL. An anticoagulant activity was observed in the fractions A, B and C of all the brown seaweeds. A procoagulant activity was observed in the fractions A, B of Cystoseira humilis and Sargassum vulgare as well as the fraction B of Padina pavonica with an indeterminate period, at 10 mg/mL.

scholarly journals Larvicidal Activity againstAedes aegyptiand Molluscicidal Activity againstBiomphalaria glabrataof Brazilian Marine Algae

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Elíca Amara Cecília Guedes ◽  
Cenira M. de Carvalho ◽  
Karlos Antonio Lisboa Ribeiro Junior ◽  
Thyago Fernando Lisboa Ribeiro ◽  
Lurdiana Dayse de Barros ◽  
...  
Keyword(s):  
Larvicidal Activity ◽  
Marine Algae ◽  
Biological Activities ◽  
Artemia Salina ◽  
Hypnea Musciformis ◽  
Toxic Compounds ◽  
Padina Gymnospora ◽  
Sargassum Vulgare ◽  
Bioassay Guided Fractionation ◽  
Molluscicidal Activity

This study investigated the biological activities of five benthic marine algae collected from Northeastern Region of Brazil. The tested activities included larvicidal activity againstAedes aegypti, molluscicidal activity againstBiomphalaria glabrata,and toxicity againstArtemia salina. Extracts ofUlva lactuca(Chlorophyta),Padina gymnospora, Sargassum vulgare(Phaeophyta),Hypnea musciformis,andDigenea simplex(Rhodophyta) were prepared using different solvents of increasing polarity, including dichloromethane, methanol, ethanol, and water. Of the extracts screened, the dichloromethane extracts ofH. musciformisandP. gymnosporaexhibited the highest activities and were subjected to bioassay-guided fractionation in hexane and chloroform. The chloroform fractions of theP. gymnosporaandH. musciformisextracts showed molluscicidal activity at values below 40 μg·mL−1(11.1460 μg·mL−1and 25.8689 μg·mL−1, resp.), and the chloroform and hexane fractions ofP. gymnosporashowed larvicidal activity at values below 40 μg·mL−1(29.018 μg·mL−1and 17.230 μg·mL−1, resp.). The crude extracts were not toxic toA. salina, whereas the chloroform and hexane fractions ofP. gymnospora(788.277 μg·mL−1and 706.990 μg·mL−1) showed moderate toxicity, indicating that the toxic compounds present in these algae are nonpolar.

scholarly journals Anticoagulant Properties from Marine Algae – A Systemic Review

2021 ◽  
Vol 12 (2) ◽  
pp. 1529-1538
Author(s):  
Pandian P ◽  
Srinivasan N
Keyword(s):  
Marine Algae ◽  
Marine Species ◽  
Arthrospira Platensis ◽  
Systemic Review ◽  
Padina Tetrastromatica ◽  
Monostroma Nitidum ◽  
Monostroma Angicava ◽  
Sargassum Tenerrimum

The anticoagulants are used to eliminate mainly blood clots. They are offered to patients who are at risk of developing clots, in order to improve their general wellbeing. Anticoagulant namely heparin, has many disadvantages like thrombocytopenia, antithrombin defensives, bleeding disorders and etc. The marine algae are sulphated polysaccharides has many advantages from commercially available anticoagulant which is derived from the animal's sources. The anticoagulant is found in various species of marine algae, mainly from the red, brown and green algae and its determined by various methods, namely APTT, PT, TT, CT. The marine algae species are Padina tetrastromatica, Ulva fasciata, Corallina, Asparagopsis taxiformis, Grateloupia filicina, Ulva rigida, Bursatella leachii, Agardhiella subulate, Turbinaria ornate, Monostroma angicava, Arthrospira platensis, Sargassum tenerrimum, Sargassum wightii, Turbinaria conoides, Lomentaria catenate, Gracilaria debilis, and Monostroma nitidum  are some of the species revealed its anticoagulant properties. The anticoagulant produced by the marine species are compared with standard namely heparin by in vitro and in vivo methods and the review result reveal that the anticoagulant values produced are nearer to the standard and in some species, the value are more than that of standard. The sample are crude extracted one and some samples are isolated from different fraction.

Attenuation of hydrogenperoxide-induced oxidative damages in L929 fibroblast cells by sulfated polysaccharides isolated from the edible marine algae Padina tetrastromatica

2019 ◽  
Vol 34 (2) ◽  
pp. 150-162 ◽  
Author(s):  
Geena Mariya Jose ◽  
Mahadevan Raghavankutty ◽  
G Muraleedhara Kurup
Keyword(s):  
Reactive Oxygen Species ◽  
Marine Algae ◽  
Reactive Oxygen ◽  
Sulfated Polysaccharides ◽  
Fibroblast Cells ◽  
Oxygen Species ◽  
Glutathione System ◽  
L929 Cells ◽  
Padina Tetrastromatica ◽  
L929 Fibroblast

Sulfated polysaccharides are polymers with potent biological roles such as antioxidant, anticancer, anti-inflammatory, and immunomodulatory activities. They are gaining significance in advanced healthcare research, since they can control oxidative cellular damages by arresting the reactive oxygen species. Hence, this study aims to evaluate the cellular antioxidant potential of sulfated polysaccharides isolated from the marine algae Padina tetrastromatica in L929 cells. The monosaccharide composition of sulfated polysaccharides was determined using high-performance liquid chromatography. The protective effect of sulfated polysaccharides on L929 fibroblast cells under H2O2-induced oxidative stress has been evaluated using MTT assay, acridine orange/ethidium bromide staining, flow cytometry, comet assay, DCFH-DA staining, fluorometry, and biochemical assays. Sulfated polysaccharides were found to contain glucose, fucose, xylose, and uronic acid. It was non-toxic to L929 cells and effectively increased the viability of mouse fibroblast cells under induced oxidative stress. Various cellular damages such as apoptosis, plasma membrane alterations, DNA damage, and lipid peroxidation induced by H2O2 have been attenuated by sulfated polysaccharides. This might be due to the reduction of intracellular reactive oxygen species level by sulfated polysaccharides as observed in DCFH-DA staining. Sulfated polysaccharides improved the activity of intracellular antioxidant status and glutathione system. The augmentation of endogenous antioxidant enzymes and glutathione system by sulfated polysaccharides can be the possible reason for the reduction of intracellular reactive oxygen species, prevention of apoptosis, and betterment of viability in L929 cells. The study confirms the potent antioxidant activity of the sulfated polysaccharides in addition to their structural and nutritional roles.

scholarly journals Protective effect of sulphated polysaccharide from Padina tetrastromatica (Dictyotaceae) on thromboembolism

2015 ◽  
Vol 7 ◽  
Author(s):  
Sulaiman Mohsin ◽  
R Mahadevan ◽  
A.S. Sumayya ◽  
G. Muraleedhara Kurup
Keyword(s):  
Platelet Aggregation ◽  
Marine Algae ◽  
In Vitro Models ◽  
Significant Inhibition ◽  
Inhibitory Effects ◽  
Antithrombotic Activity ◽  
Padina Tetrastromatica ◽  
Significant Prolongation

<p>Even though anti thrombotic effects have been reported with polysaccharides isolated from various marine algae of dictyotaceae, containing uronic acid as the main constituent. But a new polysaccharide was isolated from a species of marine algae from the Kerala coast with a composition different from those reported so far. The antiplatelet and antithrombotic activities of polysaccharides from <em>Padina tetrastromatica</em> were investigated on platelet aggregation in vitro and on pulmonary thrombosis in vivo. The polysaccharide fractions showed concentration dependent inhibitory effects on ADP-induced platelet aggregation. Using an <em>in vivo </em>mouse thrombotic model in which mice were challenged with an intravenous injection of collagen and epinephrine mixture, oral administration of the polysaccharide prior to the injection produced a significant inhibition of thrombotic death or paralysis. Aspirin showed a significant inhibition of thrombotic death similar to polysaccharide of higher dose. Polysaccharide fractions showed significant prolongation of mouse tail bleeding time. The present findings showed clear evidence of protection against thromboembolism and had good antithrombotic activity. Available data obtained by in vitro models suggest that there is a correlation between the sulfate content and antithrombotic activity.</p>

scholarly journals Marine Algae Incorporated Polylactide Acid Patch: Novel Candidate for Targeting Osteosarcoma Cells without Impairing the Osteoblastic Proliferation

Polymers ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 847
Author(s):  
Salih Veziroglu ◽  
Mustafa Ayna ◽  
Theresa Kohlhaas ◽  
Selin Sayin ◽  
Jacek Fiutowski ◽  
...  
Keyword(s):  
Material Characterization ◽  
Marine Algae ◽  
Osteosarcoma Cells ◽  
Casting Method ◽  
Collagen Matrices ◽  
Composite Patch ◽  
Sargassum Vulgare ◽  
High Production ◽  
Polylactide Acid ◽  
High Production Cost

Biodegradable collagen-based materials have been preferred as scaffolds and grafts for diverse clinical applications in density and orthopedy. Besides the advantages of using such bio-originated materials, the use of collagen matrices increases the risk of infection transmission through the cells or the tissues of the graft/scaffold. In addition, such collagen-based solutions are not counted as economically feasible approaches due to their high production cost. In recent years, incorporation of marine algae in synthetic polymers has been considered as an alternative method for preparation grafts/scaffolds since they represent abundant and cheap source of potential biopolymers. Current work aims to propose a novel composite patch prepared by blending Sargassum vulgare powders (SVP) to polylactide (PLA) as an alternative to the porcine-derived membranes. SVP-PLA composite patches were produced by using a modified solvent casting method. Following detailed material characterization to assess the cytocompatibility, human osteoblasts (HOBs) and osteosarcoma cells (SaOS-2) were seeded on neat PLA and SVP-PLA patches. MTT and BrdU assays indicated a greater cytocompatibility and higher proliferation for HOBs cultured on SVP-PLA composite than for those cultured on neat PLA. SaOS-2 cells cultured on SVP-PLA exhibited a significant decrease in cell proliferation. The composite patch described herein exhibits an antiproliferative effect against SaOS-2 cells without impairing HOBs’ adhesion and proliferation.

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