scholarly journals Profile analysis of hepatic porcine and murine brain tissue slices obtained with a vibratome

PeerJ ◽  
2015 ◽  
Vol 3 ◽  
pp. e932 ◽  
Author(s):  
G Mattei ◽  
I Cristiani ◽  
C Magliaro ◽  
A Ahluwalia
Keyword(s):  
Brain Tissue ◽  
Profile Analysis ◽  
Tissue Slices ◽  
Murine Brain

scholarly journals Profile analysis of hepatic porcine and murine brain tissue slices obtained with a vibratome

2014 ◽  
Author(s):  
Giorgio Mattei ◽  
Irene Cristiani ◽  
Chiara Magliaro ◽  
Arti Ahluwalia
Keyword(s):  
Brain Tissue ◽  
Profile Analysis ◽  
Brain Slices ◽  
Slice Thickness ◽  
Tissue Stiffness ◽  
Quantitative Investigation ◽  
Step Size ◽  
Tissue Slices ◽  
Porcine Liver ◽  
Murine Brain

This study is aimed at characterizing soft tissue slices using a vibratome. In particular, the effect of two sectioning parameters (i.e. step size and sectioning speed) on resultant slice thickness was investigated for fresh porcine liver as well as for paraformaldehyde-fixed (PFA-fixed) and fresh murine brain. A simple framework for embedding, sectioning and imaging the slices was established to derive their thickness, which was evaluated through a purposely developed graphical user interface. Sectioning speed and step size had little effect on the thickness of fresh liver slices. Conversely, the thickness of PFA-fixed murine brain slices was found to be dependent on the step size, but not on the sectioning speed. In view of these results, fresh brain tissue was sliced varying the step size only, which was found to have a significant effect on resultant slice thickness. Although precision-cut slices (i.e. with regular thickness) were obtained for all the tissues, slice accuracy (defined as the match between the nominal step size chosen and the actual slice thickness obtained) was found to increase with tissue stiffness from fresh liver to PFA-fixed brain. This quantitative investigation can be very helpful for establishing the most suitable slicing setup for a given tissue.

scholarly journals Profile analysis of hepatic porcine and murine brain tissue slices obtained with a vibratome

2014 ◽  
Author(s):  
Giorgio Mattei ◽  
Irene Cristiani ◽  
Chiara Magliaro ◽  
Arti Ahluwalia
Keyword(s):  
Brain Tissue ◽  
Profile Analysis ◽  
Brain Slices ◽  
Slice Thickness ◽  
Tissue Stiffness ◽  
Quantitative Investigation ◽  
Step Size ◽  
Tissue Slices ◽  
Porcine Liver ◽  
Murine Brain

This study is aimed at characterizing soft tissue slices using a vibratome. In particular, the effect of two sectioning parameters (i.e. step size and sectioning speed) on resultant slice thickness was investigated for fresh porcine liver as well as for paraformaldehyde-fixed (PFA-fixed) and fresh murine brain. A simple framework for embedding, sectioning and imaging the slices was established to derive their thickness, which was evaluated through a purposely developed graphical user interface. Sectioning speed and step size had little effect on the thickness of fresh liver slices. Conversely, the thickness of PFA-fixed murine brain slices was found to be dependent on the step size, but not on the sectioning speed. In view of these results, fresh brain tissue was sliced varying the step size only, which was found to have a significant effect on resultant slice thickness. Although precision-cut slices (i.e. with regular thickness) were obtained for all the tissues, slice accuracy (defined as the match between the nominal step size chosen and the actual slice thickness obtained) was found to increase with tissue stiffness from fresh liver to PFA-fixed brain. This quantitative investigation can be very helpful for establishing the most suitable slicing setup for a given tissue.

Imaging brain tissue slices with terahertz near‐field microscopy

2018 ◽  
Vol 35 (2) ◽  
pp. e2741 ◽  
Author(s):  
Guoshuai Geng ◽  
Guangbin Dai ◽  
Dandan Li ◽  
Shengling Zhou ◽  
Zaoxia Li ◽  
...  
Keyword(s):  
Brain Tissue ◽  
Near Field ◽  
Tissue Slices

Cells in human postmortem brain tissue slices remain alive for several weeks in culture

2002 ◽  
Vol 16 (1) ◽  
pp. 54-60 ◽  
Author(s):  
Ronald W. H. Verwer ◽  
Wim T. J. M. C. Hermens ◽  
Paul A. Dijkhuizen ◽  
Olivier Ter Brake ◽  
Robert E. Baker ◽  
...  
Keyword(s):  
Brain Tissue ◽  
Postmortem Brain ◽  
Tissue Slices ◽  
Human Postmortem Brain ◽  
Postmortem Brain Tissue

Single-Unit Activity of Hypothalamic Arcuate Neurons in Brain Tissue Slices

1986 ◽  
Vol 43 (2) ◽  
pp. 189-196 ◽  
Author(s):  
Jenn-Tser Pan ◽  
Lee-Ming Kow ◽  
Donald W. Pfaff
Keyword(s):  
Brain Tissue ◽  
Unit Activity ◽  
Single Unit ◽  
Single Unit Activity ◽  
Tissue Slices ◽  
Arcuate Neurons

Sample collection and amino acids analysis of extracellular fluid of mouse brain slices with low flow push–pull perfusion

The Analyst ◽  
2015 ◽  
Vol 140 (19) ◽  
pp. 6563-6570 ◽  
Author(s):  
G. Ojeda-Torres ◽  
L. Williams ◽  
D. E. Featherstone ◽  
S. A. Shippy
Keyword(s):  
Amino Acids ◽  
Brain Tissue ◽  
Mouse Brain ◽  
Brain Slices ◽  
Extracellular Fluid ◽  
Low Flow ◽  
Sample Collection ◽  
Tissue Slices ◽  
Extracellular Glutamate

Low flow push–pull perfusion is used to measure extracellular glutamate levels from mouse brain tissue slices.

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