Cells in human postmortem brain tissue slices remain alive for several weeks in culture

2002 ◽  
Vol 16 (1) ◽  
pp. 54-60 ◽  
Author(s):  
Ronald W. H. Verwer ◽  
Wim T. J. M. C. Hermens ◽  
Paul A. Dijkhuizen ◽  
Olivier Ter Brake ◽  
Robert E. Baker ◽  
...  
Keyword(s):  
Brain Tissue ◽  
Postmortem Brain ◽  
Tissue Slices ◽  
Human Postmortem Brain ◽  
Postmortem Brain Tissue

Tract tracing, neuronal morphology and ultrastructural studies in human postmortem brain

1992 ◽  
Vol 50 (1) ◽  
pp. 470-471
Author(s):  
Rosalinda C. Roberts ◽  
Michael W. Vogel ◽  
Maarten de Rijk ◽  
Frank J. Peretti ◽  
Robert R. Conley ◽  
...  
Keyword(s):  
Brain Tissue ◽  
Neuronal Morphology ◽  
Postmortem Brain ◽  
Human Brain Tissue ◽  
Tract Tracing ◽  
Human Postmortem Brain ◽  
Ultrastructural Studies ◽  
Carbocyanine Dye ◽  
Postmortem Brain Tissue ◽  
Golgi Staining

Classical and modern neuroanatomical techniques are applied to an analysis of the neuroanatomical basis of schizophrenia using postmortem brain tissue from the Maryland Brain Collection. Techniques for Golgi staining of cell bodies and dendritic arbors, axonal tract tracing with the carbocyanine dye, DiI and ultrastructural analyses will be used to study brain tissue with postmortem times of less than 6 hours. Although these techniques are routinely used in animal models, they are rarely used for studying human brain tissue. These techniques will allow us to characterize neuronal architecture in normal and diseased brains.

scholarly journals PAC1 receptor–mediated clearance of tau in postsynaptic compartments attenuates tau pathology in mouse brain

2021 ◽  
Vol 13 (595) ◽  
pp. eaba7394
Author(s):  
Ari W. Schaler ◽  
Avery M. Runyan ◽  
Catherine L. Clelland ◽  
Eric J. Sydney ◽  
Stephanie L. Fowler ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Brain Tissue ◽  
Mouse Brain ◽  
Subcellular Fractionation ◽  
Proteasome Activity ◽  
Postmortem Brain ◽  
Early Event ◽  
Pac1 Receptor ◽  
Human Postmortem Brain ◽  
Postmortem Brain Tissue

Accumulation of pathological tau in synapses has been identified as an early event in Alzheimer’s disease (AD) and correlates with cognitive decline in patients with AD. Tau is a cytosolic axonal protein, but under disease conditions, tau accumulates in postsynaptic compartments and presynaptic terminals, due to missorting within neurons, transsynaptic transfer between neurons, or a failure of clearance pathways. Using subcellular fractionation of brain tissue from rTg4510 tau transgenic mice with tauopathy and human postmortem brain tissue from patients with AD, we found accumulation of seed-competent tau predominantly in postsynaptic compartments. Tau-mediated toxicity in postsynaptic compartments was exacerbated by impaired proteasome activity detected by measuring lysine-48 polyubiquitination of proteins targeted for proteasomal degradation. To combat the accumulation of tau and proteasome impairment in the postsynaptic compartments of rTg4510 mouse brain, we stimulated the pituitary adenylate cyclase–activating polypeptide (PACAP) type 1 receptor (PAC1R) with its ligand PACAP administered intracerebroventricularly to rTg4510 mice. We observed enhanced synaptic proteasome activity and reduced total tau in postsynaptic compartments in mouse brain after PACAP treatment. The clearance of tau from postsynaptic compartments correlated with attenuated tauopathy and improved cognitive performance of rTg4510 transgenic mice on two behavioral tests. These results suggest that activating PAC1R could prevent accumulation of aggregate-prone tau and indicate a potential therapeutic approach for AD and other tauopathies.

scholarly journals Tryptophan is a marker of human postmortem brain tissue quality

2009 ◽  
Vol 110 (5) ◽  
pp. 1400-1408 ◽  
Author(s):  
Edna Grünblatt ◽  
Camelia Maria Monoranu ◽  
Manuela Apfelbacher ◽  
Daniela Keller ◽  
Tanja M. Michel ◽  
...  
Keyword(s):  
Brain Tissue ◽  
Postmortem Brain ◽  
Tissue Quality ◽  
Human Postmortem Brain ◽  
Postmortem Brain Tissue
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