Single-Unit Activity of Hypothalamic Arcuate Neurons in Brain Tissue Slices

1986 ◽  
Vol 43 (2) ◽  
pp. 189-196 ◽  
Author(s):  
Jenn-Tser Pan ◽  
Lee-Ming Kow ◽  
Donald W. Pfaff
Keyword(s):  
Brain Tissue ◽  
Unit Activity ◽  
Single Unit ◽  
Single Unit Activity ◽  
Tissue Slices ◽  
Arcuate Neurons

Effects of ouabain on neuronal thermosensitivity in hypothalamic tissue slices

1989 ◽  
Vol 257 (1) ◽  
pp. R21-R28
Author(s):  
M. C. Curras ◽  
J. A. Boulant
Keyword(s):  
Firing Rate ◽  
Neuronal Activity ◽  
Unit Activity ◽  
Single Unit ◽  
Single Unit Activity ◽  
Tissue Slices ◽  
Temperature Changes ◽  
Cold Sensitive ◽  
Hypothalamic Tissue

To determine the role of the electrogenic Na+-K+ pump in neuronal thermosensitivity, single-unit activity was recorded in rat hypothalamic tissue slices before, during, and after perfusions containing 10(-5) or 10(-6) M ouabain, a specific pump inhibitor. Most neurons were recorded in the preoptic-anterior hypothalamus. Some neurons were also tested with high magnesium-low calcium perfusions to determine ouabain's effects on neuronal activity during synaptic blockade. When the neurons were characterized according to thermosensitivity, 24% were warm sensitive, 8% were cold sensitive, and 68% were temperature insensitive. Ouabain increased the firing rate of 60% of all neurons. Ouabain did not reduce the thermosensitivity of cold-sensitive and warm-sensitive neurons; however, temperature-insensitive neurons became more warm sensitive during ouabain perfusion. This increase in warm sensitivity did not occur with ouabain plus high Mg2+-low Ca2+ perfusion, suggesting that Ca2+ is important in this response. These results indicate that the Na-K pump is not responsible for the thermosensitivity of hypothalamic cold-sensitive or warm-sensitive neurons; however, this pump may be actively employed by many neurons that remain insensitive to temperature changes.

Thermosensitive single-unit activity of in vitro hypothalamic slices

1982 ◽  
Vol 242 (1) ◽  
pp. R77-R84 ◽  
Author(s):  
S. R. Kelso ◽  
M. N. Perlmutter ◽  
J. A. Boulant
Keyword(s):  
Firing Rate ◽  
Unit Activity ◽  
Single Unit ◽  
Animal Studies ◽  
Afferent Input ◽  
Single Unit Activity ◽  
Tissue Slices ◽  
Cold Sensitive ◽  
Inhibitory Synaptic Input

Single-unit activity was recorded in vitro from tissue slices of rat preoptic area-anterior hypothalamus. The thermosensitivity of 139 units was determined by their changes in firing rate in response to changes in slice temperature. Of these neurons, 30% were warm sensitive, 10% were cold sensitive, and 60% were temperature insensitive. These proportions are similar to results obtained in whole-animal studies, indicating that this is a viable preparation. It also suggests that hypothalamic neuronal thermosensitivity is not dependent on peripheral afferent input. All units had low firing rates (less than 10 imp/s) at 37 degrees C, and 83% of the warm-sensitive units were most thermosensitive above 37 degrees C. This supports the concept that afferent input determines the level of firing rate and range of thermosensitivity of warm-sensitive neurons. The cold-sensitive units also displayed maximal thermosensitivity above 37 degrees C, which would be expected if cold-sensitive neurons received inhibitory synaptic input from nearby warm-sensitive neurons.

Effect of Eye Opening on Single-Unit Activity and Local Field Potentials in the Subthalamic Nucleus

2017 ◽  
Vol 20 (5) ◽  
pp. 471-477 ◽  
Author(s):  
Arun Chockalingam ◽  
Abigail Belasen ◽  
Nita Chen ◽  
Adolfo Ramirez-Zamora ◽  
Youngwon Youn ◽  
...  
Keyword(s):  
Subthalamic Nucleus ◽  
Local Field ◽  
Unit Activity ◽  
Single Unit ◽  
Local Field Potentials ◽  
Single Unit Activity ◽  
Field Potentials ◽  
Eye Opening
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