Polymerase chain reaction (PCR) diagnosis of human brucellosis (l7/l12 and 16srRNA genes) compared with immunocapture-agglutination test (brucellacapt) and common serological tests

2012 ◽  
Vol 6 (26) ◽  
Author(s):  
Pakzad I.,
Keyword(s):  
Polymerase Chain Reaction ◽  
Agglutination Test ◽  
Human Brucellosis ◽  
Chain Reaction ◽  
Serological Tests ◽  
Pcr Diagnosis ◽  
Polymerase Chain

scholarly journals Comparison of multiplex real-time polymerase chain reaction with serological tests and culture for diagnosing human brucellosis

2019 ◽  
Vol 12 (3) ◽  
pp. 337-342 ◽  
Author(s):  
Tuba Dal ◽  
Soner Sertan Kara ◽  
Aytekin Cikman ◽  
Cigdem Eda Balkan ◽  
Ziya Cibali Acıkgoz ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Human Brucellosis ◽  
Chain Reaction ◽  
Serological Tests ◽  
Polymerase Chain

scholarly journals Seroprevalence of brucellosis in patients with prolonged fever in Bangladesh

2016 ◽  
Vol 10 (09) ◽  
pp. 939-946 ◽  
Author(s):  
AKM Anisur Rahman ◽  
Dirk Berkvens ◽  
Claude Saegerman ◽  
David Fretin ◽  
Noor Muhammad ◽  
...  
Keyword(s):  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Brucellosis ◽  
Chain Reaction ◽  
Serological Tests ◽  
Human Sera ◽  
Polymerase Chain ◽  
Species Specific ◽  
First Time ◽  
Sera Samples

Introduction: This study describes the seroprevalence of human brucellosis among pyretic patients and detection of Brucella abortus DNA from seropositive pyretic patients using real-time polymerase chain reaction (rtPCR) for the first time in Bangladesh. Methodology: Blood samples were collected from 300 pyretic patients from October 2007 to May 2008 and subjected to three serological tests: Rose-Bengal plate test (RBT), standard tube agglutination test (STAT), and indirect enzyme-linked immunosorbent assay (iELISA). Risk factors were identified by multivariate Firth’s logistic regression analysis. Brucella genus (BCSP31) and species-specific (IS711) rtPCR were applied to six human sera samples. Results: The seroprevalence of brucellosis among pyretic patients was estimated to be 2.0% (95% confidence interval [CI]: 0.74–4.30). The odds of brucellosis seropositivity were 8.9 (95% CI: 1.26–63.0) times higher in pyretic patients who handled goats than those who handled only cattle, whereas the odds of brucellosis seropositivity were 9.7 (95% CI: 1.28–73.68) times higher in pyretic patients who had backache compared to those without backache. B. abortus DNA was amplified from all six human sera that tested positive by RBT, STAT, and iELISA. As the agreement between the tests was very strong, RBT is recommended as a screening test for the diagnosis of human brucellosis in Bangladesh because it is easier to use, cheaper, and faster. Conclusions: Brucellosis among pyretic patients is common, and B. abortus is responsible for brucellosis in such patients. Pyretic patients who handle goats and those with backaches should be screened for brucellosis.

scholarly journals USE OF THE POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF ASYMPTOMATIC Leishmania INFECTION IN A VISCERAL LEISHMANIASIS-ENDEMIC AREA

2013 ◽  
Vol 55 (2) ◽  
pp. 101-104 ◽  
Author(s):  
Luciana Almeida Silva ◽  
Héctor Dardo Romero ◽  
Aline Fagundes ◽  
Nédia Nehme ◽  
Otávio Fernandes ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Visceral Leishmaniasis ◽  
Endemic Area ◽  
Asymptomatic Infection ◽  
Control Measures ◽  
Skin Tests ◽  
Leishmania Infection ◽  
Chain Reaction ◽  
Serological Tests ◽  
Polymerase Chain

The diagnosis of asymptomatic infection with Leishmania (Leishmania) chagasi has become more important over recent years. Expansion of visceral leishmaniasis might be associated with other routes of transmission such as transfusion, congenital or even vector transmission, and subjects with asymptomatic infection are potential reservoirs. Moreover, the identification of infection may contribute to the management of patients with immunosuppressive conditions (HIV, transplants, use of immunomodulators) and to the assessment of the effectiveness of control measures. In this study, 149 subjects living in a visceral leishmaniasis endemic area were evaluated clinically and submitted to genus-specific polymerase chain reaction (PCR), serological testing, and the Montenegro skin test. Forty-nine (32.9%) of the subjects had a positive PCR result and none of them developed the disease within a follow-up period of three years. No association was observed between the results of PCR, serological and skin tests. A positive PCR result in subjects from the endemic area did not indicate a risk of progression to visceral leishmaniasis and was not associated with a positive result in the serological tests.

scholarly journals Polymerase chain reaction–based assays for the diagnosis of human brucellosis

2014 ◽  
Vol 13 (1) ◽  
Author(s):  
Ying Wang ◽  
Zhanli Wang ◽  
Yaxian Zhang ◽  
Liyun Bai ◽  
Yue Zhao ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Brucellosis ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Molecular Typing of Human Brucella melitensis Isolated from Patients in Erbil, Iraq

2019 ◽  
Vol 7 (1) ◽  
pp. 1 ◽  
Author(s):  
Bushra K. Amin ◽  
Khulod I. Hassan
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Typing ◽  
Brucella Melitensis ◽  
Control Strategies ◽  
Human Brucellosis ◽  
Kurdistan Region ◽  
Specific Primer ◽  
Molecular Method ◽  
Chain Reaction ◽  
Polymerase Chain

Brucellosis is a reemerging infectious zoonotic disease of worldwide importance. In the Kurdistan Region of Iraq, it is a widely spread disease and remains a challenging health problem. This disease is mainly caused by Brucella melitensis, in human. For confirmation of these isolates, a study was performed, by isolation and molecular typing of Brucella Spp. from human patients in Rizgari Hospital at Erbil city (Iraq), between March 2014 and November 2016. One hundred sixty seven samples of blood collected from patients suspected for brucellosis, one hundred twenty one samples from these were recorded as genus of Brucella, using biochemical test and confirmed by applying polymerase chain reaction (PCR), using genus specific primer for omp31 gene which was specific for B. melitensis. These results support using molecular method that based on PCR as diagnostic test for the control of brucellosis in Erbil. Further studies are needed from different geographical areas of the country with different level of endemicity to plan and execute control strategies against human brucellosis.

scholarly journals Diagnosis of Brucellosis in Recently Aborted Ewes Using Serological Tests and Polymerase Chain Reaction

2019 ◽  
Vol 19 (2) ◽  
pp. 77-81 ◽  
Author(s):  
Hassan Ali Hussei ◽  
Ragab Hassan Moh ◽  
Aml Mohammed A ◽  
Eman Mahmoud Ab ◽  
Rasha Salah Moha ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Serological Tests ◽  
Polymerase Chain

scholarly journals Previous Syphilis Alters the Course of Subsequent Episodes of Syphilis

2019 ◽  
Vol 71 (5) ◽  
pp. 1243-1247 ◽  
Author(s):  
Christina M Marra ◽  
Clare L Maxwell ◽  
Sharon K Sahi ◽  
Lauren C Tantalo ◽  
Shelia B Dunaway ◽  
...  
Keyword(s):  
Public Health ◽  
Polymerase Chain Reaction ◽  
Cerebrospinal Fluid ◽  
Logistic Regression ◽  
Treponema Pallidum ◽  
Chain Reaction ◽  
Serological Tests ◽  
Health Records ◽  
Polymerase Chain ◽  
Diagnosis Date

Abstract Background Individuals with previous syphilis may be more likely to be asymptomatic when they are reinfected with Treponema pallidum. Methods Individuals enrolled in a study of cerebrospinal fluid (CSF) abnormalities in syphilis were allowed to enroll in the study again with subsequent syphilis. For each participant, the index episode was defined as the most recent syphilis episode for which the study entry visit was performed within 30 days of the syphilis diagnosis date. Venipuncture and lumbar puncture were performed. The total number of syphilis episodes was determined by review of medical and public health records. Treponema pallidum DNA in blood and rRNA in CSF were detected using polymerase chain reaction (PCR) and reverse transcriptase PCR. Odds ratios (ORs) with 95% confidence intervals (CIs) were determined using logistic regression. Results 701 individuals had 1 (n = 478), 2 (n = 155), or ≥3 (n = 68) episodes of syphilis. The proportion of individuals whose index episode was asymptomatic significantly increased with increased number of syphilis episodes (P < .001). This difference was not explained by frequency of serological tests. Adjusted ORs (aORs) of detection of T. pallidum DNA in blood or rRNA in CSF at the index episode were significantly lower in those with previous syphilis (0.13; 95% CI, .08–.23, and 0.06, 95% CI, .02–.17). The aOR of neurosyphilis at the index episode was also significantly lower in individuals with previous syphilis (0.43; 95% CI, .27–.68). Conclusions Previous syphilis attenuates clinical and laboratory manifestations of infection with T. pallidum.

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