scholarly journals Molecular Typing of Human Brucella melitensis Isolated from Patients in Erbil, Iraq

2019 ◽  
Vol 7 (1) ◽  
pp. 1 ◽  
Author(s):  
Bushra K. Amin ◽  
Khulod I. Hassan
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Typing ◽  
Brucella Melitensis ◽  
Control Strategies ◽  
Human Brucellosis ◽  
Kurdistan Region ◽  
Specific Primer ◽  
Molecular Method ◽  
Chain Reaction ◽  
Polymerase Chain

Brucellosis is a reemerging infectious zoonotic disease of worldwide importance. In the Kurdistan Region of Iraq, it is a widely spread disease and remains a challenging health problem. This disease is mainly caused by Brucella melitensis, in human. For confirmation of these isolates, a study was performed, by isolation and molecular typing of Brucella Spp. from human patients in Rizgari Hospital at Erbil city (Iraq), between March 2014 and November 2016. One hundred sixty seven samples of blood collected from patients suspected for brucellosis, one hundred twenty one samples from these were recorded as genus of Brucella, using biochemical test and confirmed by applying polymerase chain reaction (PCR), using genus specific primer for omp31 gene which was specific for B. melitensis. These results support using molecular method that based on PCR as diagnostic test for the control of brucellosis in Erbil. Further studies are needed from different geographical areas of the country with different level of endemicity to plan and execute control strategies against human brucellosis.

scholarly journals Comparison of multiplex real-time polymerase chain reaction with serological tests and culture for diagnosing human brucellosis

2019 ◽  
Vol 12 (3) ◽  
pp. 337-342 ◽  
Author(s):  
Tuba Dal ◽  
Soner Sertan Kara ◽  
Aytekin Cikman ◽  
Cigdem Eda Balkan ◽  
Ziya Cibali Acıkgoz ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Human Brucellosis ◽  
Chain Reaction ◽  
Serological Tests ◽  
Polymerase Chain

scholarly journals Streptococcus agalactiae mastitis of bovine detection by Polymerase Chain Reaction (PCR) test in AL-Diwanyia province

2013 ◽  
Vol 12 (2) ◽  
pp. 101
Author(s):  
Gh. K. A. Al-kuzaay ◽  
Q. H. Kshash
Keyword(s):  
Polymerase Chain Reaction ◽  
Streptococcus Agalactiae ◽  
Specific Primer ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Milk Samples ◽  
Bacteriological Testing ◽  
Polymerase Chain ◽  
Pcr Test

This study was conducted for exam 348 milk samples from (clinically mastitic and other healthy cows) in many areas in AL-Diwanyia province by using CMT and bacteriological testing , which appeared that (64.9%) as percentage of mastitis ( clinically 15.9% , subclinically 84.0% ) Streptococcus agalactiae mastitis 13.2% ( 26.6% clinically , 73.3 % subclinicaly) diagnose by PCR assay by using specific primer (16SrRNA). Streptococcus agalactiae (30 isolates) after classical methods applied for streptococcus agalactiae identification (86 isolates).

Polymerase chain reaction-based detection of dermatophyte DNA with a fungus-specific primer system

Mycoses ◽  
2009 ◽  
Vol 37 (3-4) ◽  
pp. 79-84 ◽  
Author(s):  
M. Bock ◽  
M. Maiwald ◽  
R. Kappe ◽  
P. Nickel ◽  
H. Näher
Keyword(s):  
Polymerase Chain Reaction ◽  
Specific Primer ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Simplified polymerase chain reaction (PCR)-based sexing assists conservation of an endangered owl, the Norfolk Island Boobook Ninox novaeseelandiae undulata

1997 ◽  
Vol 7 (3) ◽  
pp. 283-286 ◽  
Author(s):  
Michael Double ◽  
Penny Olsen
Keyword(s):  
Polymerase Chain Reaction ◽  
New Zealand ◽  
Second Generation ◽  
Molecular Method ◽  
Chain Reaction ◽  
Norfolk Island ◽  
In The Wild ◽  
Polymerase Chain ◽  
Recovery Effort ◽  
Main Factor

In 1986 a single Norfolk Island Boobook Owl Ninox novaeseelandiae undulata remained. As part of a re-establishment programme, two male New Zealand Moreporks N. n. novaeseelandiae were introduced, one of which survived to pair with the female in the wild and breed successfully. By 1995 the population numbered 12 or 13 individuals of which seven were second generation (F2). However, there were only two breeding pairs. As the 11 hybrids could not be sexed using morphometrics we developed a molecular method based on a recently described avian polymerase chain reaction (PCR)-based sexing technique. The population was found to contain six females and five males. A scarcity of mature males was established as the main factor slowing the recovery effort.

scholarly journals The Inc FII Plasmid and its Contribution in the Transmission of blaNDM-1 and blaKPC-2 in Klebsiella pneumoniae in Egypt

Antibiotics ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 266 ◽  
Author(s):  
Eman Ramadan Mohamed ◽  
Mamdouh Yones Ali ◽  
Nancy G F M Waly ◽  
Hamada Mohamed Halby ◽  
Rehab Mahmoud Abd El-Baky
Keyword(s):  
Polymerase Chain Reaction ◽  
Klebsiella Pneumoniae ◽  
Molecular Typing ◽  
University Hospital ◽  
Chain Reaction ◽  
Great Problem ◽  
E Coli ◽  
String Test ◽  
Resistance Patterns ◽  
Polymerase Chain

The emergence of blaKPC-2 and blaNDM-1 producing Klebsiella pneumoniae represents a great problem in many Egyptian hospitals. One hundred and twenty-six K. pneumoniae isolates from patients admitted to Assiut University Hospital were identified by an API20E kit. Carbapenemase-producing K. pneumoniae (CPKP) was detected by the modified carbapenem inactivation method (mCIM), the EDTA-modified carbapenem inactivation method (eCIM), and an E-test. Based on the polymerase chain reaction, all isolates were negative for bla-VIM-1 and bla-IMP-1, fifteen of these isolates were positive for both blaKPC-2 and blaNDM-1, two isolates were positive for blaKPC-2 only, and twenty-eight isolates were positive for bla-NDM-1 only. Although one isolate was positive for the string test, all CPKP isolates were negative for capsular genes. Only 71.1% of CPKP transferred their plasmids to their corresponding transconjugants (E. coli J53). The resistance patterns of the clinical isolates and their transconjugates were similar, except for 12 isolates, which showed differences with their transconjugates in the resistance profile of four antibiotics. Molecular typing of the plasmids based on replicon typing showed that Inc FIIK and FII plasmids predominated in isolates and their transconjugants carrying blaKPC-2 and/or blaNDM-1. Conjugative Inc FII plasmids play an important role in the spread of CPKP, and their recognition is essential to limit their spread.

scholarly journals Molecular Epidemiology of Rhodococcus equi Based on traA, vapA, and vapB Virulence Plasmid Markers

2007 ◽  
Vol 196 (5) ◽  
pp. 763-769 ◽  
Author(s):  
Alain A Ocampo-Sosa ◽  
Deborah A Lewis ◽  
Jesús Navas ◽  
Frances Quigley ◽  
Raquel Callejo ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Typing ◽  
Rhodococcus Equi ◽  
Virulence Plasmid ◽  
Chain Reaction ◽  
Gene Markers ◽  
Typing System ◽  
New Type ◽  
Polymerase Chain ◽  
Related Plasmid

AbstractMolecular typing of the actinomycete Rhodococcus equi is insufficiently developed, and little is known about the epidemiology and transmission of this multihost pathogen. We report a simple, reliable polymerase chain reaction typing system for R. equi based on 3 plasmid gene markers: traA from the conserved conjugal transfer machinery and vapA and vapB, found in 2 different plasmid subpopulations. This “TRAVAP” typing scheme classifies R. equi into 4 categories: traA+/vapA+B−, traA+/vapA−B+, traA+/vapAB−, and traA−/vapAB− (plasmidless). A TRAVAP survey of 215 R. equi strains confirmed the strong link between vapA (traA+/vapA+B− plasmids) and horse isolates and revealed other host-related plasmid associations: between traA+/vapA−B+ and pigs and between traA+/vapAB−—a new type of R. equi plasmid—and cattle. Plasmidless strains were more frequent among isolates from nonpathological specimens. All plasmid categories were common in human isolates, which possibly reflects the predominantly opportunistic nature of R. equi infection in this host and a zoonotic origin.

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