scholarly journals Comparison of multiplex real-time polymerase chain reaction with serological tests and culture for diagnosing human brucellosis

2019 ◽  
Vol 12 (3) ◽  
pp. 337-342 ◽  
Author(s):  
Tuba Dal ◽  
Soner Sertan Kara ◽  
Aytekin Cikman ◽  
Cigdem Eda Balkan ◽  
Ziya Cibali Acıkgoz ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Human Brucellosis ◽  
Chain Reaction ◽  
Serological Tests ◽  
Polymerase Chain

scholarly journals Molecular Characteristics of the Serological Weak D Phenotype in Koreans

Diagnostics ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 920
Author(s):  
Dajeong Jeong ◽  
Sujin Oh ◽  
Eun Young Song ◽  
Yun Ji Hong ◽  
Kyoung Un Park
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Characteristics ◽  
Chain Reaction ◽  
Serological Tests ◽  
Molecular Tests ◽  
Specific Amplification ◽  
Polymerase Chain

Serological weak D is a reaction of 2+ or less to anti-D reagent and includes weak D and partial D phenotypes. Although identifying the RhD subtype is important for transfusion safety, serological tests are insufficient for defining the RhD subtype, and molecular tests are needed. To analyze the molecular characteristics of D variants in Koreans to facilitate the formulation of individualized transfusion strategies, molecular tests such as RhD genotyping using real-time polymerase chain reaction (PCR) and partial-D and/or weak-D sequence-specific amplification (SSP) were performed on 105 Korean Rare Blood Program (KRBP) patients exhibiting serological weak D. In total, 58 out of 68 serologically determined weak D KRBP patients were typed as having weak D or partial D phenotypes via RhD genotyping. In detail, eight (13.8%) were typed as partial DVa or DBS, nine (15.5%) as weak D type 15, and four others (6.8%) as partial DVI, partial DVII, weak D type 2, or weak D type 41 or 45, whereas the rest (n = 37, 63.8%) was typed as having either weak D or partial D. This suggests that serological weak D Koreans who require transfusion should be treated as D-negative.

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain
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