Differential nitrate accumulation, nitrate reduction, nitrate reductase activity, protein production and carbohydrate biosynthesis in response to potassium and sodium nitrate

2011 ◽  
Vol 10 (78) ◽  
Author(s):  
Sadegh Balotf
Keyword(s):  
Nitrate Reductase ◽  
Sodium Nitrate ◽  
Nitrate Reduction ◽  
Nitrate Reductase Activity ◽  
Protein Production ◽  
Reductase Activity ◽  
Nitrate Accumulation

Differential effects of ozone on in vivo nitrate reduction in soybean cultivars. I. Response to exogenous sugars

1978 ◽  
Vol 56 (13) ◽  
pp. 1540-1544 ◽  
Author(s):  
Albert C. Purvis
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reduction ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Foliar Injury ◽  
Glycolytic Intermediates ◽  
Glycine Max L ◽  
Growth Chambers ◽  
Visible Foliar Injury

Two cultivars of soybeans (Glycine max (L.) Merr.) differing widely in their resistance to ozone were exposed to 0.5 μl/ℓ ozone for 2 h in growth chambers. In vivo nitrate reduction was depressed by more than 50% in the primary leaves of Dare, the ozone-sensitive cultivar, but was not significantly altered in Hood, the ozone-resistant cultivar. Sucrose, up to 1.5% (w/v), added to excised seedlings of the Dare cultivar during exposure to ozone eliminated the ozone depression of in vivo nitrate reductase activity and also reduced foliar injury. Addition of two glycolytic intermediates, glyceraldehyde-3-phosphate and fructose-1,6-diphosphate, to the infiltration medium recovered some in vivo nitrate reduction in treated Dare leaves. The levels of extractable nitrate reductase and glyceraldehyde-3-phosphate dehydrogenase in the primary leaves of both cultivars were unaltered by ozone fumigations. These observations led to the conclusion that ozone depression of in vivo nitrate reduction is not due to ozone inactivation of nitrate reductase or of the enzymes coupling nitrate reduction to glycolysis, but may be caused by an inadequate supply of photosynthetic sugars. It was also noted that ozone depression of in vivo nitrate reduction only occurred with treatments which subsequently caused the development of visible foliar injury.

scholarly journals Respiratory Nitrate Ammonification by Shewanella oneidensis MR-1

2006 ◽  
Vol 189 (2) ◽  
pp. 656-662 ◽  
Author(s):  
Claribel Cruz-García ◽  
Alison E. Murray ◽  
Joel A. Klappenbach ◽  
Valley Stewart ◽  
James M. Tiedje
Keyword(s):  
Nitrous Oxide ◽  
Nitrate Reductase ◽  
Electron Acceptor ◽  
Nitrate Reduction ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Shewanella Oneidensis ◽  
Gene Encoding ◽  
Nitrate Ammonification ◽  
Sequential Reduction

ABSTRACT Anaerobic cultures of Shewanella oneidensis MR-1 grown with nitrate as the sole electron acceptor exhibited sequential reduction of nitrate to nitrite and then to ammonium. Little dinitrogen and nitrous oxide were detected, and no growth occurred on nitrous oxide. A mutant with the napA gene encoding periplasmic nitrate reductase deleted could not respire or assimilate nitrate and did not express nitrate reductase activity, confirming that the NapA enzyme is the sole nitrate reductase. Hence, S. oneidensis MR-1 conducts respiratory nitrate ammonification, also termed dissimilatory nitrate reduction to ammonium, but not respiratory denitrification.

Effets d'un éclairage d'appoint sur la croissance et le métabolisme azoté primaire de la laitue mâche et de l'épinard cultivés en serre

1999 ◽  
Vol 79 (3) ◽  
pp. 421-426 ◽  
Author(s):  
Hassan Chadjaâ ◽  
Louis-Philippe Vézina ◽  
André Gosselin
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Climatic Conditions ◽  
Nitrate Accumulation ◽  
Artificial Lighting ◽  
Spinacea Oleracea ◽  
Supplementary Lighting ◽  
Nitrates Content ◽  
High Yields

Two cultivars of Lamb's lettuce (Valerianella sp. 'Valgros' et 'Vit') and one cultivar of spinach (Spinacea oleracea L. 'Martine RZ F1') were subjected to three light treatments. Two photoperiods, 12 h and 16 h were compared to natural light between January and April 1994. A photosynthetic flux of 50 µmol m–2 s–1 was provided as supplementary lighting using HPS lamps. The use of artificial lighting significantly increased biomass and nitrate reductase activity, while also reducing nitrate accumulation in leaves. The 16 h-photoperiod increased biomass and reduced nitrates content in leaves more than the 12 h photoperiod. In Lamb's lettuce, Valgos was more productive than Vit but accumulated more nitrates. The use of supplementary lighting gave high yields of lamb's lettuce and spinach in greenhouse under northern climatic conditions. Key words: Lamb's lettuce, spinach, nitrate reductase activity, nitrate, Valerianella sp., Spinacea oleracea L.

scholarly journals Nitrate Accumulation and Reduction in Papaw Fruits

1972 ◽  
Vol 25 (3) ◽  
pp. 531 ◽  
Author(s):  
RC Menary ◽  
RH Jones
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Phloem Transport ◽  
Fruit Maturation ◽  
Ripe Fruit ◽  
Nitrate Accumulation ◽  
Xylem Transport ◽  
Colour Changes ◽  
Green Stage

Nitrate reductase activity was present in roots, stems, leaves, and fruits of the papaw. In fruits, the location and activity of nitrate reductase varied with stage of maturation. Nitrate reductase activity was induced in the exocarp of green and mature green fruits by light and light plus exogenous nitrate. The endogenous nitrate in the exocarp of ripe fruit was not available for reduction and hence no induction occurred in the presence of light only. As fruit tissues comll1enced to show colour changes, there was a marked loss in their nitrate reductase activity. At the mature green stage of maturity, phloem transport appeared to predominate over xylem transport and this reduced the net influx of nitrate to fruit. The levels of nitrate observed in fruits at various stages of maturity may be explained in terms of the following: changes in nitrate reductase activity associated with fruit maturation; the availability of endogenous nitrate for reduction; factors controlling the net influx of nitrate into fruits and distribution of nitrate within fruits.

Nitrate reductase activities of rhizobia and the correlation between nitrate reduction and nitrogen fixation

1979 ◽  
Vol 25 (10) ◽  
pp. 1169-1174 ◽  
Author(s):  
James R. Manhart ◽  
Peter P. Wong
Keyword(s):  
Nitrogen Fixation ◽  
Nitrate Reductase ◽  
Nitrate Reduction ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Root Nodules ◽  
Sole Source ◽  
Rhizobium Japonicum ◽  
Rhizobium Trifolii ◽  
Rhizobium Species

All species of Rhizobium except R. lupini had nitrate reductase activity. Only R. lupini was incapable of growth with nitrate as the sole source of nitrogen. However, the conditions necessary for the induction of nitrate reductase varied among species of Rhizobium. Rhizobium japonicum and some Rhizobium species of the cowpea strains expressed nitrate reductase activities both in the root nodules of appropriate leguminous hosts and when grown in the presence of nitrate. Rhizobium trifolii, R. phaseoli, and R. legnminosarum did not express nitrate reductase activities in the root nodules, but they did express them when grown in the presence of nitrate. In bacteroids of R. japonicum and some strains of cowpea Rhizobium, high N2 fixation activities were accompanied by high nitrate reductase activities. In bacteroids of R. trifolii, R. leguminosarum, and R. phaseoli, high N2 fixation activities were not accompanied by high nitrate reductase activities.

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