Discrepancy between nitrate reduction rates in intact leaves and nitrate reductase activity in leaf extracts: What limits nitrate reduction in situ?

Planta ◽  
2000 ◽  
Vol 210 (5) ◽  
pp. 801-807 ◽  
Author(s):  
Werner M. Kaiser ◽  
Andrea Kandlbinder ◽  
Maria Stoimenova ◽  
Johanna Glaab
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reduction ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Leaf Extracts ◽  
Intact Leaves

Differential effects of ozone on in vivo nitrate reduction in soybean cultivars. I. Response to exogenous sugars

1978 ◽  
Vol 56 (13) ◽  
pp. 1540-1544 ◽  
Author(s):  
Albert C. Purvis
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reduction ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Foliar Injury ◽  
Glycolytic Intermediates ◽  
Glycine Max L ◽  
Growth Chambers ◽  
Visible Foliar Injury

Two cultivars of soybeans (Glycine max (L.) Merr.) differing widely in their resistance to ozone were exposed to 0.5 μl/ℓ ozone for 2 h in growth chambers. In vivo nitrate reduction was depressed by more than 50% in the primary leaves of Dare, the ozone-sensitive cultivar, but was not significantly altered in Hood, the ozone-resistant cultivar. Sucrose, up to 1.5% (w/v), added to excised seedlings of the Dare cultivar during exposure to ozone eliminated the ozone depression of in vivo nitrate reductase activity and also reduced foliar injury. Addition of two glycolytic intermediates, glyceraldehyde-3-phosphate and fructose-1,6-diphosphate, to the infiltration medium recovered some in vivo nitrate reduction in treated Dare leaves. The levels of extractable nitrate reductase and glyceraldehyde-3-phosphate dehydrogenase in the primary leaves of both cultivars were unaltered by ozone fumigations. These observations led to the conclusion that ozone depression of in vivo nitrate reduction is not due to ozone inactivation of nitrate reductase or of the enzymes coupling nitrate reduction to glycolysis, but may be caused by an inadequate supply of photosynthetic sugars. It was also noted that ozone depression of in vivo nitrate reduction only occurred with treatments which subsequently caused the development of visible foliar injury.

In situ nitrate reductase activity as an indicator of nitrate availability

1987 ◽  
Vol 102 (1) ◽  
pp. 145-148 ◽  
Author(s):  
L. Passama ◽  
A. Gojon ◽  
P. Robin ◽  
L. Salsac
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Nitrate Availability

scholarly journals Respiratory Nitrate Ammonification by Shewanella oneidensis MR-1

2006 ◽  
Vol 189 (2) ◽  
pp. 656-662 ◽  
Author(s):  
Claribel Cruz-García ◽  
Alison E. Murray ◽  
Joel A. Klappenbach ◽  
Valley Stewart ◽  
James M. Tiedje
Keyword(s):  
Nitrous Oxide ◽  
Nitrate Reductase ◽  
Electron Acceptor ◽  
Nitrate Reduction ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Shewanella Oneidensis ◽  
Gene Encoding ◽  
Nitrate Ammonification ◽  
Sequential Reduction

ABSTRACT Anaerobic cultures of Shewanella oneidensis MR-1 grown with nitrate as the sole electron acceptor exhibited sequential reduction of nitrate to nitrite and then to ammonium. Little dinitrogen and nitrous oxide were detected, and no growth occurred on nitrous oxide. A mutant with the napA gene encoding periplasmic nitrate reductase deleted could not respire or assimilate nitrate and did not express nitrate reductase activity, confirming that the NapA enzyme is the sole nitrate reductase. Hence, S. oneidensis MR-1 conducts respiratory nitrate ammonification, also termed dissimilatory nitrate reduction to ammonium, but not respiratory denitrification.

scholarly journals Influence of deproteinised foliage fluid liquid biofertilizer on nitrate reductase activity of Eleusine coracana plants

2018 ◽  
pp. 33-36 ◽  
Author(s):  
Rajesh K. Jadhav ◽  
Gare Chhaya
Keyword(s):  
Nitrate Reductase ◽  
Spectrophotometric Method ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Eleusine Coracana ◽  
Wheat Crop ◽  
Leaf Extracts ◽  
Cassia Tora ◽  
Number Of Leaves ◽  
Leaf Juice

Deproteinised leaf extracts previously found favourably enhancing plant and fungi growth. Present attempt has been made to observe its influence in enhancing the enzyme nitrate reductase. Crop of Eleusine coracana was cultivated  and  treated  by  deproteined  whey  from  the  leguminous  weed Cassia tora  and  its  influence examined on the  activity of  the  enzyme  nitrate  reductase  by  spectrophotometric method. Cassia tora  crop  was fractionated  for  the  purpose  to  isolate  crude  leaf  protein by  heating the juice  to  90°C  which gave  deproteinised  leaf  juice (DPJ). The crop growth was also compared with other monocotyledonous wheat,  Sorghum and  bajra   crops.  There  was  enhancement of nitrate  reductase  in  wheat  crop  by the application of  non  leguminous  Basella forage DPJ. There  was  lesser  activity  of  nitrate  reductase in  Eleusine  crop  by the influence  of Cassia  tora  DPJ probably because  of  the  deficiency  of  element  molybdenum in  the utilised  red  soil  for  the   growth  of  crops or allelopathy. Length  and  number  of  leaves of  Eleusine crop was  measured. 

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