Targeting the human lysozyme gene on bovine αs1-casein gene locus in fibroblasts

2011 ◽  
Vol 10 (75) ◽  
Author(s):  
Gong Cheng
Keyword(s):  
Gene Locus ◽  
Human Lysozyme ◽  
Casein Gene ◽  
Lysozyme Gene

Organization of the bovine casein gene locus

1997 ◽  
Vol 8 (2) ◽  
pp. 148-152 ◽  
Author(s):  
M. Rijnkels ◽  
P. M. Kooiman ◽  
H. A. de Boer ◽  
F. R. Pieper
Keyword(s):  
Gene Locus ◽  
Casein Gene

Unusual clustering ofAlurepeats within the 5'-flanking region of the human lysozyme gene

DNA Sequence ◽  
1993 ◽  
Vol 4 (2) ◽  
pp. 129-134 ◽  
Author(s):  
Maria Letizia Riccio ◽  
Gian Maria Rossolini
Keyword(s):  
Human Lysozyme ◽  
Lysozyme Gene ◽  
Flanking Region

REPRODUCTIVE ABILITY OF COWS WITH DIFFERENT GENOTYPES OF DAIRY GENES

2015 ◽  
Vol 10 (2) ◽  
pp. 101-104
Author(s):  
Сибагатуллин ◽  
Fatikh Sibagatullin ◽  
Шайдуллин ◽  
Radik Shaydullin ◽  
Ганиев ◽  
...  
Keyword(s):  
Body Weight ◽  
Gene Locus ◽  
Reproductive Capacity ◽  
Early Age ◽  
Lower Body ◽  
Casein Gene ◽  
Reproductive Ability ◽  
Lower Body Weight

The aim of this work was to study the reproductive capacity of fresh cows with various genotypes of kappa-casein gene and diatsetilglitserin O-acetyl transferase. It was revealed that at an early age of insemination and calving, animals have a lower body weight at first calving. Cows on the gene locus of kappa-casein AB and BB, gene of butterfat AK and KK were observed elongated expectancy service- and between calving periods (106 and 394 days, 105 and 400 days) and a lower coefficient of reproductive ability and Doha’s index (0.93 and 46.2, 0.91 and 45.8), which suggests a slight decrease in their reproductive capacity.

High level expression of the synthetic human lysozyme gene in Aspergillus oryzae

1992 ◽  
Vol 38 (1) ◽  
Author(s):  
Kozo Tsuchiya ◽  
Setsuzo Tada ◽  
Katsuya Gomi ◽  
katsuhiko Kitamoto ◽  
Chieko Kumagai ◽  
...  
Keyword(s):  
Aspergillus Oryzae ◽  
High Level Expression ◽  
Human Lysozyme ◽  
Lysozyme Gene ◽  
High Level

scholarly journals The human lysozyme gene. Sequence organization and chromosomal localization

1989 ◽  
Vol 182 (3) ◽  
pp. 507-516 ◽  
Author(s):  
Christoph W. B. PETERS ◽  
Ulrich KRUSE ◽  
Renate POLLWEIN ◽  
Karl-Heinz GRZESCHIK ◽  
Albrecht E. SIPPEL
Keyword(s):  
Gene Sequence ◽  
Chromosomal Localization ◽  
Human Lysozyme ◽  
Sequence Organization ◽  
Lysozyme Gene

scholarly journals Expression of synthetic human lysozyme gene in Escherichia coli.

1985 ◽  
Vol 49 (9) ◽  
pp. 2829-2831 ◽  
Author(s):  
Michiro MURAKI ◽  
Yoshifumi JIGAMI ◽  
Hideaki TANAKA ◽  
Fumitaka KISHIMOTO ◽  
Hideo AGUI ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Human Lysozyme ◽  
Lysozyme Gene

Creation of Transgenic Bananas Expressing Human Lysozyme Gene for Panama Wilt Resistance

2005 ◽  
Vol 47 (8) ◽  
pp. 971-977 ◽  
Author(s):  
Xin-Wu PEI ◽  
Shi-Kai CHEN ◽  
Rui-Ming WEN ◽  
Shang YE ◽  
Jia-Qin HUANG ◽  
...  
Keyword(s):  
Human Lysozyme ◽  
Wilt Resistance ◽  
Lysozyme Gene

209 PRODUCTION OF BUFFALO (BUBALUS BUBALIS) EMBRYOS CONTAINING HUMAN LYSOZYME GENE

2017 ◽  
Vol 29 (1) ◽  
pp. 213
Author(s):  
A. Kumar ◽  
R. Kaushik ◽  
P. Mehta ◽  
K. P. Singh ◽  
M. S. Chauhan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Animal Health ◽  
Inflammatory Factor ◽  
Fibroblast Cells ◽  
Human Lysozyme ◽  
Transfected Cells ◽  
Lysozyme Gene ◽  
Immune Factor ◽  
Blastocyst Rate ◽  
Transgenic Embryos

Lysozyme is a ubiquitous enzyme found in all major taxa of living organisms with a diverse role in human and animal health. It plays an important role as a nonspecific immune factor and anti-inflammatory factor and is a part of the innate immune system. Research on the expression of recombinant human lysozyme is, thus, potentially valuable to the dairy industry. Therefore, the present study was carried out to observe the developmental competence and quality of cloned embryos containing the human lysozyme gene. Primary fetal fibroblast cells were obtained from a slaughterhouse-derived fetus. The initial passage cells were transfected with pAchLYZ vector containing human lysozyme gene and green fluorescence protein (GFP) via nucleofection, lipofectamine, and FuGene (Roche Diagnostics, Indianapolis, IN, USA). Transfected cells were selected by adding G418 (400–800 µg µL−1), which selectively killed the nontransfected cells in culture. The presence of hLYZ gene in transfected cells was confirmed by PCR amplification of this gene. For cloned embryo production, reconstructs were formed with 2 enucleated demi oocyte fused with 1 donor cell of hLYZ transfected cells or nontransfected fibroblast cells. Gene expression in the resulting embryos was assessed for apoptosis (BID, BAX, and BCL-XL) and development- (G6PD, IGF1R, and FGF) and pluripotency-related (OCT4, SOX2, and NANOG) genes. The transfection efficiency of the cells by nucleofection methods was the highest and toxicity to the cells was minimum as compared with chemical methods. Transfected cells expressed GFP within 48 to 72 h of transfection. When these cells were passaged the intensity of GFP expression was reduced and the reduced level was maintained in subsequent passages. A total of 114 and 60 reconstructed embryos were produced using transfected and nontransfected cells, respectively. When transfected cells were used, the cleavage, 4 cell, 8 to 16 cell, morula, and blastocyst rate was 62.01 ± 4.78, 43.54 ± 3.93, 27.69 ± 4.44, 24.55 ± 5.12, and 22.87 ± 5.39%, respectively, whereas rates were 73.34 ± 2.36, 68.34 ± 4.82, 59.89 ± 2.39, 46.44 ± 2.64, and 36.36 ± 2.53% for nontransfected cells, respectively. The cleavage rate had no significant (P < 0.05, Student’s t-test) difference in both type of donor cells but all other stages [i.e. 4 cell, 8 to 16 cell, morula, and blastocyst rate was significantly (P < 0.05) lower with transfected cells]. Most of the transgenic cloned embryos expressed GFP and integrated with hLYZ gene. We found that proapoptotic gene (BID and BAX) expression did not differ, whereas BCL-XL expression was significantly low (P < 0.05) in transgenic embryos. The development- (G6PD, IGF1R, and FGF) and pluripotency-related (OCT4, SOX2, and NANOG) gene expression was significantly (P < 0.05) lower in transgenic embryos. In conclusion, transgenic cloned embryo successfully developed up to the blastocyst stage in the preliminary study for producing genetically modified animal with human milk components having antimicrobial activity, which would be potentially valuable for human as well as animal health.

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