scholarly journals Expression of synthetic human lysozyme gene in Escherichia coli.

1985 ◽  
Vol 49 (9) ◽  
pp. 2829-2831 ◽  
Author(s):  
Michiro MURAKI ◽  
Yoshifumi JIGAMI ◽  
Hideaki TANAKA ◽  
Fumitaka KISHIMOTO ◽  
Hideo AGUI ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Human Lysozyme ◽  
Lysozyme Gene

scholarly journals Expression of synthetic human lysozyme gene in Escherichia coli.

1986 ◽  
Vol 50 (3) ◽  
pp. 713-723 ◽  
Author(s):  
Michiro MURAKI ◽  
Yoshifumi JIGAMI ◽  
Hideaki TANAKA ◽  
Nobuhiro HARADA ◽  
Fumitaka KISHIMOTO ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Human Lysozyme ◽  
Lysozyme Gene

scholarly journals Expression of Synthetic Human Lysozyme Gene in Escherichia coli

1985 ◽  
Vol 49 (9) ◽  
pp. 2829-2831 ◽  
Author(s):  
Michiro Muraki ◽  
Yoshifumi Jigami ◽  
Hideaki Tanaka ◽  
Fumitaka Kishimoto ◽  
Hideo Agui ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Human Lysozyme ◽  
Lysozyme Gene

scholarly journals Overexpression of Trigger Factor Prevents Aggregation of Recombinant Proteins in Escherichia coli

2000 ◽  
Vol 66 (3) ◽  
pp. 884-889 ◽  
Author(s):  
Kazuyo Nishihara ◽  
Masaaki Kanemori ◽  
Hideki Yanagi ◽  
Takashi Yura
Keyword(s):  
Escherichia Coli ◽  
Recombinant Proteins ◽  
Protein Production ◽  
Trigger Factor ◽  
Human Lysozyme ◽  
E Coli ◽  
Constructed Plasmids ◽  
Expression Plasmids

ABSTRACT To examine the effects of overexpression of trigger factor (TF) on recombinant proteins produced in Escherichia coli, we constructed plasmids that permitted controlled expression of TF alone or together with the GroEL-GroES chaperones. The following three proteins that are prone to aggregation were tested as targets: mouse endostatin, human oxygen-regulated protein ORP150, and human lysozyme. The results revealed that TF overexpression had marked effects on the production of these proteins in soluble forms, presumably through facilitating correct folding. Whereas overexpression of TF alone was sufficient to prevent aggregation of endostatin, overexpression of TF together with GroEL-GroES was more effective for ORP150 and lysozyme, suggesting that TF and GroEL-GroES play synergistic roles in vivo. Although coexpression of the DnaK-DnaJ-GrpE chaperones was also effective for endostatin and ORP150, coexpression of TF and GroEL-GroES was more effective for lysozyme. These results attest to the usefulness of the present expression plasmids for improving protein production inE. coli.

Unusual clustering ofAlurepeats within the 5'-flanking region of the human lysozyme gene

DNA Sequence ◽  
1993 ◽  
Vol 4 (2) ◽  
pp. 129-134 ◽  
Author(s):  
Maria Letizia Riccio ◽  
Gian Maria Rossolini
Keyword(s):  
Human Lysozyme ◽  
Lysozyme Gene ◽  
Flanking Region

High level expression of the synthetic human lysozyme gene in Aspergillus oryzae

1992 ◽  
Vol 38 (1) ◽  
Author(s):  
Kozo Tsuchiya ◽  
Setsuzo Tada ◽  
Katsuya Gomi ◽  
katsuhiko Kitamoto ◽  
Chieko Kumagai ◽  
...  
Keyword(s):  
Aspergillus Oryzae ◽  
High Level Expression ◽  
Human Lysozyme ◽  
Lysozyme Gene ◽  
High Level

scholarly journals The human lysozyme gene. Sequence organization and chromosomal localization

1989 ◽  
Vol 182 (3) ◽  
pp. 507-516 ◽  
Author(s):  
Christoph W. B. PETERS ◽  
Ulrich KRUSE ◽  
Renate POLLWEIN ◽  
Karl-Heinz GRZESCHIK ◽  
Albrecht E. SIPPEL
Keyword(s):  
Gene Sequence ◽  
Chromosomal Localization ◽  
Human Lysozyme ◽  
Sequence Organization ◽  
Lysozyme Gene
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