Organization of the bovine casein gene locus

1997 ◽  
Vol 8 (2) ◽  
pp. 148-152 ◽  
Author(s):  
M. Rijnkels ◽  
P. M. Kooiman ◽  
H. A. de Boer ◽  
F. R. Pieper
Keyword(s):  
Gene Locus ◽  
Casein Gene

REPRODUCTIVE ABILITY OF COWS WITH DIFFERENT GENOTYPES OF DAIRY GENES

2015 ◽  
Vol 10 (2) ◽  
pp. 101-104
Author(s):  
Сибагатуллин ◽  
Fatikh Sibagatullin ◽  
Шайдуллин ◽  
Radik Shaydullin ◽  
Ганиев ◽  
...  
Keyword(s):  
Body Weight ◽  
Gene Locus ◽  
Reproductive Capacity ◽  
Early Age ◽  
Lower Body ◽  
Casein Gene ◽  
Reproductive Ability ◽  
Lower Body Weight

The aim of this work was to study the reproductive capacity of fresh cows with various genotypes of kappa-casein gene and diatsetilglitserin O-acetyl transferase. It was revealed that at an early age of insemination and calving, animals have a lower body weight at first calving. Cows on the gene locus of kappa-casein AB and BB, gene of butterfat AK and KK were observed elongated expectancy service- and between calving periods (106 and 394 days, 105 and 400 days) and a lower coefficient of reproductive ability and Doha’s index (0.93 and 46.2, 0.91 and 45.8), which suggests a slight decrease in their reproductive capacity.

Structure and expression of the mouse casein gene locus

1997 ◽  
Vol 8 (1) ◽  
pp. 9-15 ◽  
Author(s):  
M. Rijnkels ◽  
D. A. Wheeler ◽  
H. A. de Boer ◽  
F. R. Pieper
Keyword(s):  
Gene Locus ◽  
Casein Gene

Physical map and localization of the human casein gene locus

1997 ◽  
Vol 8 (4) ◽  
pp. 285-286 ◽  
Author(s):  
M. Rijnkels ◽  
E. Meershoek ◽  
H. A. de Boer ◽  
F. R. Pieper
Keyword(s):  
Gene Locus ◽  
Physical Map ◽  
Casein Gene

Knock-in Vector for Expression of Insulin-like Growth Factor 1 on the Bovine β-casein Gene Locus

2017 ◽  
Vol 41 (3) ◽  
pp. 51-55
Author(s):  
Sang Young Kim ◽  
Da Som Park ◽  
Se Eun Kim ◽  
Man-Jong Kang ◽  
Deog-Bon Koo
Keyword(s):  
Growth Factor ◽  
Gene Locus ◽  
Insulin Like Growth Factor ◽  
Casein Gene

Knock-in fibroblasts and transgenic blastocysts for expression of human FGF2 in the bovine β-casein gene locus using CRISPR/Cas9 nuclease-mediated homologous recombination

Zygote ◽  
2015 ◽  
Vol 24 (3) ◽  
pp. 442-456 ◽  
Author(s):  
Young-Hee Jeong ◽  
Yeong Ji Kim ◽  
Eun Young Kim ◽  
Se Eun Kim ◽  
Jiwoo Kim ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Gene Locus ◽  
Mammary Epithelial Cells ◽  
Culture Media ◽  
Expression System ◽  
Therapeutic Proteins ◽  
Mammary Epithelial ◽  
Vector System ◽  
Specific Gene ◽  
Casein Gene

SummaryMany transgenic domestic animals have been developed to produce therapeutic proteins in the mammary gland, and this approach is one of the most important methods for agricultural and biomedical applications. However, expression and secretion of a protein varies because transgenes are integrated at random sites in the genome. In addition, distal enhancers are very important for transcriptional gene regulation and tissue-specific gene expression. Development of a vector system regulated accurately in the genome is needed to improve production of therapeutic proteins. The objective of this study was to develop a knock-in system for expression of human fibroblast growth factor 2 (FGF2) in the bovine β-casein gene locus. The F2A sequence was fused to the human FGF2 gene and inserted into exon 3 of the β-casein gene. We detected expression of human FGF2 mRNA in the HC11 mouse mammary epithelial cells by RT-PCR and human FGF2 protein in the culture media using western blot analysis when the knock-in vector was introduced. We transfected the knock-in vector into bovine ear fibroblasts and produced knock-in fibroblasts using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system. Moreover, the CRISPR/Cas9 system was more efficient than conventional methods. In addition, we produced knock-in blastocysts by somatic cell nuclear transfer using the knock-in fibroblasts. Our knock-in fibroblasts may help to create cloned embryos for development of transgenic dairy cattle expressing human FGF2 protein in the mammary gland via the expression system of the bovine β-casein gene.

Characterization of rabbit κ-casein cDNA: control of κ-casein gene expression in vivo and in vitro

1993 ◽  
Vol 11 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Zs Bösze ◽  
E Devinoy ◽  
C Puissant ◽  
M L Fontaine ◽  
L M Houdebine
Keyword(s):  
Gene Expression ◽  
Mammary Gland ◽  
Gene Locus ◽  
Phosphorylation Sites ◽  
Casein Gene ◽  
Mouse Sequence ◽  
Partial Cdna

ABSTRACT The rabbit κ-casein cDNA was cloned and sequenced. One of the isolated clones included almost the entire 5′ end, while another clone corresponded to the 3′ end of the cDNA. No polyadenylation site was found and therefore this clone did not harbour the complete cDNA. The amino acid sequence of a full-length protein was deduced from the nucleotide sequence obtained for this partial cDNA. It revealed the presence of a chymosin cleavage site and five potential phosphorylation sites. Rabbit κ-casein was compared with those already described in other species. The rabbit sequence is closer to the ovine than to the mouse sequence. This result supports the idea that Lagomorpha are not closer to Rodentia than to Artiodactyla. The cDNA described above was used to study κ-casein gene expression in the rabbit mammary gland. This expression was induced primarily by prolactin in mammary gland organoids and was similar to αs1-casein gene expression in vivo. The κ-casein gene present in the casein gene locus is thus subject to the same regulation as the αs1-casein gene, although it has evolved from a fibrinogen gene.

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