scholarly journals Cloning and functional expression of a human lysozyme gene (hly) from human leukocytes in Pichia pastoris

2012 ◽  
Author(s):  
Min-Chen Wu
Keyword(s):  
Pichia Pastoris ◽  
Functional Expression ◽  
Human Lysozyme ◽  
Human Leukocytes ◽  
Lysozyme Gene

SECRETED EXPRESSION OF LITOPENAEUS VANNAMEI LYSOZYME GENE IN PICHIA PASTORIS AND ITS BIOACTIVITY DETECTION

2010 ◽  
Vol 36 (6) ◽  
pp. 1091-1096
Author(s):  
Shu-Guang BIAN ◽  
Hua-Xin CHEN ◽  
Peng JIANG ◽  
Hai-Bo ZHANG ◽  
Zhao-Pu LIU ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Litopenaeus Vannamei ◽  
Lysozyme Gene ◽  
Secreted Expression

Enhanced human lysozyme production by Pichia pastoris via periodic glycerol and dissolved oxygen concentrations control

2021 ◽  
Vol 105 (3) ◽  
pp. 1041-1050
Author(s):  
Luqiang Jia ◽  
Teng Li ◽  
Yixuan Wu ◽  
Chunsen Wu ◽  
Huaxiang Li ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Dissolved Oxygen ◽  
Human Lysozyme ◽  
Oxygen Concentrations

Functional expression of porcine interferon-α using a combinational strategy in Pichia pastoris GS115

2019 ◽  
Vol 122 ◽  
pp. 55-63 ◽  
Author(s):  
Huahua He ◽  
Chao Zhai ◽  
Meng Mei ◽  
Yi Rao ◽  
Yao Liu ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Functional Expression ◽  
Interferon Α ◽  
Pastoris Gs115 ◽  
Pichia Pastoris Gs115

Cloning, functional expression and characterization of Aspergillus sulphureus β-mannanase in Pichia pastoris

2007 ◽  
Vol 128 (3) ◽  
pp. 452-461 ◽  
Author(s):  
Xiaoling Chen ◽  
Yunhe Cao ◽  
Yuhua Ding ◽  
Wenqing Lu ◽  
Defa Li
Keyword(s):  
Pichia Pastoris ◽  
Functional Expression ◽  
Aspergillus Sulphureus

Unusual clustering ofAlurepeats within the 5'-flanking region of the human lysozyme gene

DNA Sequence ◽  
1993 ◽  
Vol 4 (2) ◽  
pp. 129-134 ◽  
Author(s):  
Maria Letizia Riccio ◽  
Gian Maria Rossolini
Keyword(s):  
Human Lysozyme ◽  
Lysozyme Gene ◽  
Flanking Region

scholarly journals Functional expression of the lactate permease Jen1p of Saccharomyces cerevisiae in Pichia pastoris

2003 ◽  
Vol 376 (3) ◽  
pp. 781-787 ◽  
Author(s):  
Isabel SOARES-SILVA ◽  
Dorit SCHULLER ◽  
Raquel P. ANDRADE ◽  
Fátima BALTAZAR ◽  
Fernanda CÁSSIO ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acetic Acid ◽  
Lactic Acid ◽  
Pichia Pastoris ◽  
Plasma Membranes ◽  
Membrane Vesicle ◽  
Dry Weight ◽  
Functional Expression ◽  
Acid Uptake ◽  
Methanol Induction

In Saccharomyces cerevisiae the activity for the lactate–proton symporter is dependent on JEN1 gene expression. Pichia pastoris was transformed with an integrative plasmid containing the JEN1 gene. After 24 h of methanol induction, Northern and Western blotting analyses indicated the expression of JEN1 in the transformants. Lactate permease activity was obtained in P. pastoris cells with a Vmax of 2.1 nmol·s−1·mg of dry weight−1. Reconstitution of the lactate permease activity was achieved by fusing plasma membranes of P. pastoris methanol-induced cells with Escherichia coli liposomes containing cytochrome c oxidase, as proton-motive force. These assays in reconstituted heterologous P. pastoris membrane vesicles demonstrate that S. cerevisiae Jen1p is a functional lactate transporter. Moreover, a S. cerevisiae strain deleted in the JEN1 gene was transformed with a centromeric plasmid containing JEN1 under the control of the glyceraldehyde-3-phosphate dehydrogenase constitutive promotor. Constitutive JEN1 expression and lactic acid uptake were observed in cells grown on either glucose and/or acetic acid. The highest Vmax (0.84 nmol·s−1·mg of dry weight−1) was obtained in acetic acid-grown cells. Thus overexpression of the S. cerevisiae JEN1 gene in both S. cerevisiae and P. pastoris cells resulted in increased activity of lactate transport when compared with the data previously reported in lactic acid-grown cells of native S. cerevisiae strains. Jen1p is the only S. cerevisiae secondary porter characterized so far by heterologous expression in P. pastoris at both the cell and the membrane-vesicle levels.

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