A case report on peritoneum mesothelioma-Effective application of cell block method and cell transfer method to body cavity fluid cytological analysis-

2009 ◽  
Vol 48 (4) ◽  
pp. 181-186
Author(s):  
Kaname SUZUMURA ◽  
Shotaro MAEDA ◽  
Masaru HOSONE ◽  
Hironori KATAYAMA ◽  
Keiko AZUMA ◽  
...  
Keyword(s):  
Case Report ◽  
Body Cavity ◽  
Cell Transfer ◽  
Cell Block ◽  
Cytological Analysis ◽  
Block Method ◽  
Effective Application ◽  
Transfer Method

Rapid Cell Transfer by Means of Nylon Mesh to Improve Cellular Diagnosis: The Role of Immunocytochemistry

2021 ◽  
pp. 1-6
Author(s):  
Satoshi Morito ◽  
Takao Nitanda ◽  
Ryuko Tsukamoto ◽  
Shingo Kamoshida ◽  
Hiroshi Yasui ◽  
...  
Keyword(s):  
Needle Aspiration ◽  
Turnaround Time ◽  
Body Cavity ◽  
Cell Transfer ◽  
Special Equipment ◽  
Nylon Mesh ◽  
Cytological Smear ◽  
Liquid Based Cytology ◽  
Transfer Method

Immunocytochemistry (ICC) is an important ancillary technique in clinical cytology for not only identifying and characterizing tumor cells but also gaining prognostic or therapeutic information. Although cell blocks are often prepared for immunocytochemical evaluation of body cavity fluid and fine-needle aspiration specimens, they are not suitable for hypocellular samples. Liquid-based cytology can help prepare additional smears from residual cytological specimens. However, since conventional methods are used for nongynecological specimens in most laboratories, ICC is often limited by the number of cytological smears. Cell transfer methods permit to evaluate several immunocytochemical markers in a single cytological smear. Yet, these methods have some limitations; for example, they are time-consuming (about 3–40 h) and medium membranes with their attached cells are occasionally stretched or torn when peeled off the slides. Therefore, in an attempt to solve these problems, we developed a rapid and reliable cell transfer method using a nylon mesh. Our method requires no special equipment or reagent and can significantly reduce the turnaround time, as compared to previous methods.

scholarly journals A STUDY OF BODY CAVITY EFFUSIONS AND ITS DIAGNOSTIC UTILITY OF CELL BLOCK AS AN ADJUNCT TO CONVENTIONAL CYTOLOGICAL SMEARS

2020 ◽  
pp. 10-11
Author(s):  
Ankan Nandi ◽  
Debarshi Jana
Keyword(s):  
Observational Study ◽  
Body Cavity ◽  
Diagnostic Utility ◽  
Cytological Diagnosis ◽  
Cell Block ◽  
Tissue Architecture ◽  
Block Method ◽  
Exclusion Criteria ◽  
Cross Sectional ◽  
Malignant Effusions

Background: The cell block technique is one of the oldest method for evaluation of body cavity fluids and it increases the sensitivity of the diagnosis in comparison to conventional smear study. The main advantage of cell block technique is preservation of tissue architecture and obtaining multiple sections for special stains. Aim: To assess the utility of cell block method in increasing the sensitivity of cytological diagnosis of body cavity effusions as an adjunct to conventional smear method. Material and methods: It was institution based cross sectional observational study. All patients presenting with effusion and those who was give consent for participating in the study was included in this study. The study was conducted in Dept. Of Pathology, Nandigram Superspeciality Hospital. 100 cases fulfilling the predetermined inclusion and exclusion criteria was taken during the study period. Result: Association of Cellularity vs cell block was statistically significant (p<0.0001). Association of Architectural Pattern vs cell block was not statistically significant (p=0.7883). Association of cytological smears vs cell block was statistically significant (p=<0.0001). Conclusion: Cell block technique is superior to conventional smear technique, especially for malignant effusions.

scholarly journals Analysis of diagnostic value of cytological smear method versus cell block method in body fluids with clinical and biochemical correlation: study of 150 cases

2021 ◽  
Vol 7 (1) ◽  
pp. 9-16
Author(s):  
Dr. Rajesh h. Chandan ◽  
◽  
Dr. Sumana Pawar ◽  
Dr Purushotham Redd ◽  
◽  
...  
Keyword(s):  
Primary Source ◽  
Diagnostic Value ◽  
Correlation Study ◽  
Body Cavity ◽  
Body Fluids ◽  
Invasive Technique ◽  
Cell Block ◽  
Block Method ◽  
Cytological Smear ◽  
Significant Difference

Background: Aspiration of serous cavities is a simple and relatively non-invasive technique toachieve diagnosis. Cytological evaluation of body cavity fluid is diagnostically challenging. Especiallyin malignant effusions, helps in staging, prognosis and management of the patients. Aims: Toassess the utility and sensitivity of cell block method over conventional smear technique incytodiagnosis of the serous effusions. And to assess the utility and sensitivity of cytologicalevaluation of body fluids with biochemical and clinical correlation. Methods: A total of 150 fluidspecimens were examined for conventional cytological smear (CS) and cell block method (CB). Outof 150 fluids, 96 were pleural fluid, 48 were ascitic fluid, 04 fluid from pouch of Douglas and 01 wasfrom synovial fluid. Results: In this study, the utility of the CB method in the cytodiagnosis ofmalignant effusions was found to be highly significant as compared to the CS method. The additionalyield of malignancy was 12% more as was obtained by the CB method. Conclusion: For the finalcytodiagnosis of body fluid, there is statistically significant difference between the two techniques. Inother words, CB is superior to CS method. It gives more information about the architecturalarrangement and the likely source of primary. More important is that diagnostic material in cellblocks is available for special studies for Immunohistochemistry which can further supplement ourknowledge about the primary source of metastasis.

scholarly journals Furuncular Botfly Myiasis – A Case Report

2018 ◽  
Vol 10 (1) ◽  
pp. 9-11
Author(s):  
Juliana Gao ◽  
Vera Tešić ◽  
Vesna Petronić Rosić
Keyword(s):  
Case Report ◽  
Life Cycle ◽  
South America ◽  
Treatment Options ◽  
Local Population ◽  
Body Cavity ◽  
Parasite Life Cycle ◽  
Susceptible Host ◽  
Dermatobia Hominis ◽  
Blood Sucking

Abstract Botfly myiasis is an infestation of the skin or a body cavity by developing larvae of Dermatobia hominis, one of the most common flies that cause human infestation among the local population, in regions ranging from Mexico into South America and in travelers. The life cycle starts when a female fly glues the eggs to the vector, a blood-sucking arthropod, which carries the unhatched larvae to the susceptible host. A case of furuncular botfly myiasis in an 85 year-old female with recent travel to Belize is presented here to highlight the parasite life cycle and review the different treatment options.

The cell block method is useful for predicting the primary site of cancer in cases of peritonitis carcinomatosa

2021 ◽  
Vol 60 (2) ◽  
pp. 86-93
Author(s):  
Yoshitomo KONISHI ◽  
Satoko KAGABU ◽  
Iwao ONO ◽  
Akira ISHII ◽  
Hiroto KON ◽  
...  
Keyword(s):  
Primary Site ◽  
Cell Block ◽  
Block Method ◽  
Peritonitis Carcinomatosa

scholarly journals Mucinous carcinoma originating in the peritoneum diagnosed by an ascites cell block: a case report

2020 ◽  
Vol 14 (1) ◽  
Author(s):  
Sachiko Nagao ◽  
Motoki Matsuura ◽  
Masato Tamate ◽  
Shintaro Sugita ◽  
Tsuyoshi Saito
Keyword(s):  
Case Report ◽  
Mucinous Carcinoma ◽  
Cell Block ◽  
Ascites Cell

New Cell Block Method to Enhance the Cellular Yield in Mucous and/or Bloody Samples

2019 ◽  
Vol 64 (3) ◽  
pp. 265-269 ◽  
Author(s):  
Heli Hakso-Mäkinen ◽  
Ivana Kholová
Keyword(s):  
Diagnostic Accuracy ◽  
Study Design ◽  
Diagnostic Utility ◽  
Cytological Diagnosis ◽  
Cell Block ◽  
Block Method

Objective: Cell blocks (CBs) are used to complement cytological diagnosis and for ancillary testing. Dissatisfaction with the cellular yield of the CB is widely recognized. Various techniques have been developed to increase the diagnostic utility of CBs. Study Design: We invented a new CB technique to increase cellular yield and diagnostic accuracy suitable especially for mucous and/or bloody cytological samples. Results: The new CB technique is described in detail with illustrations and cases, where it increased the cellular yield and diagnostic accuracy. CBs prepared by this method are suitable also for ancillary techniques, namely immunocytochemistry. Conclusions: The newly described method showed a better cellular yield in mucous and/or bloody cytological specimens.

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