Definitive diagnosis of mesothelioma by effusion cytology-Use of the cell transfer method or cell block method for immunochemical staining-

2008 ◽  
Vol 47 (2) ◽  
pp. 103-110 ◽  
Author(s):  
Shotaro MAEDA ◽  
Masaru HOSONE ◽  
Hironori KATAYAMA ◽  
Hiromi IWASE ◽  
Kiyoko KAWANO ◽  
...  
Keyword(s):  
Definitive Diagnosis ◽  
Cell Transfer ◽  
Cell Block ◽  
Block Method ◽  
Immunochemical Staining ◽  
Transfer Method

Rapid Cell Transfer by Means of Nylon Mesh to Improve Cellular Diagnosis: The Role of Immunocytochemistry

2021 ◽  
pp. 1-6
Author(s):  
Satoshi Morito ◽  
Takao Nitanda ◽  
Ryuko Tsukamoto ◽  
Shingo Kamoshida ◽  
Hiroshi Yasui ◽  
...  
Keyword(s):  
Needle Aspiration ◽  
Turnaround Time ◽  
Body Cavity ◽  
Cell Transfer ◽  
Special Equipment ◽  
Nylon Mesh ◽  
Cytological Smear ◽  
Liquid Based Cytology ◽  
Transfer Method

Immunocytochemistry (ICC) is an important ancillary technique in clinical cytology for not only identifying and characterizing tumor cells but also gaining prognostic or therapeutic information. Although cell blocks are often prepared for immunocytochemical evaluation of body cavity fluid and fine-needle aspiration specimens, they are not suitable for hypocellular samples. Liquid-based cytology can help prepare additional smears from residual cytological specimens. However, since conventional methods are used for nongynecological specimens in most laboratories, ICC is often limited by the number of cytological smears. Cell transfer methods permit to evaluate several immunocytochemical markers in a single cytological smear. Yet, these methods have some limitations; for example, they are time-consuming (about 3–40 h) and medium membranes with their attached cells are occasionally stretched or torn when peeled off the slides. Therefore, in an attempt to solve these problems, we developed a rapid and reliable cell transfer method using a nylon mesh. Our method requires no special equipment or reagent and can significantly reduce the turnaround time, as compared to previous methods.

The cell block method is useful for predicting the primary site of cancer in cases of peritonitis carcinomatosa

2021 ◽  
Vol 60 (2) ◽  
pp. 86-93
Author(s):  
Yoshitomo KONISHI ◽  
Satoko KAGABU ◽  
Iwao ONO ◽  
Akira ISHII ◽  
Hiroto KON ◽  
...  
Keyword(s):  
Primary Site ◽  
Cell Block ◽  
Block Method ◽  
Peritonitis Carcinomatosa

New Cell Block Method to Enhance the Cellular Yield in Mucous and/or Bloody Samples

2019 ◽  
Vol 64 (3) ◽  
pp. 265-269 ◽  
Author(s):  
Heli Hakso-Mäkinen ◽  
Ivana Kholová
Keyword(s):  
Diagnostic Accuracy ◽  
Study Design ◽  
Diagnostic Utility ◽  
Cytological Diagnosis ◽  
Cell Block ◽  
Block Method

Objective: Cell blocks (CBs) are used to complement cytological diagnosis and for ancillary testing. Dissatisfaction with the cellular yield of the CB is widely recognized. Various techniques have been developed to increase the diagnostic utility of CBs. Study Design: We invented a new CB technique to increase cellular yield and diagnostic accuracy suitable especially for mucous and/or bloody cytological samples. Results: The new CB technique is described in detail with illustrations and cases, where it increased the cellular yield and diagnostic accuracy. CBs prepared by this method are suitable also for ancillary techniques, namely immunocytochemistry. Conclusions: The newly described method showed a better cellular yield in mucous and/or bloody cytological specimens.

scholarly journals Preparation of frozen cell-block sections amenableto diagnostic immunocytochemistry: a technical report on cryo-embedded cell-block method

2020 ◽  
Vol 71 (2) ◽  
pp. 146-154
Author(s):  
Joo Lee ◽  
Suk Choi ◽  
Lucia Kim ◽  
In Park ◽  
Jee Han ◽  
...  
Keyword(s):  
Cell Block ◽  
Block Method ◽  
Technical Report

DIAGNOSTIC UTILITY OF CELL BLOCK METHOD IN PLEURAL FLUID CYTOLOGY

2014 ◽  
Vol 1 (9) ◽  
pp. 1240-1245
Author(s):  
Gayathri M N ◽  
Kunal Puri ◽  
Satish M K ◽  
Ravikumar T ◽  
Bharathi M
Keyword(s):  
Pleural Fluid ◽  
Diagnostic Utility ◽  
Cell Block ◽  
Block Method ◽  
Fluid Cytology
Sign in / Sign up

Export Citation Format

Share Document