New Cell Block Method to Enhance the Cellular Yield in Mucous and/or Bloody Samples

2019 ◽  
Vol 64 (3) ◽  
pp. 265-269 ◽  
Author(s):  
Heli Hakso-Mäkinen ◽  
Ivana Kholová
Keyword(s):  
Diagnostic Accuracy ◽  
Study Design ◽  
Diagnostic Utility ◽  
Cytological Diagnosis ◽  
Cell Block ◽  
Block Method

Objective: Cell blocks (CBs) are used to complement cytological diagnosis and for ancillary testing. Dissatisfaction with the cellular yield of the CB is widely recognized. Various techniques have been developed to increase the diagnostic utility of CBs. Study Design: We invented a new CB technique to increase cellular yield and diagnostic accuracy suitable especially for mucous and/or bloody cytological samples. Results: The new CB technique is described in detail with illustrations and cases, where it increased the cellular yield and diagnostic accuracy. CBs prepared by this method are suitable also for ancillary techniques, namely immunocytochemistry. Conclusions: The newly described method showed a better cellular yield in mucous and/or bloody cytological specimens.

scholarly journals A STUDY OF BODY CAVITY EFFUSIONS AND ITS DIAGNOSTIC UTILITY OF CELL BLOCK AS AN ADJUNCT TO CONVENTIONAL CYTOLOGICAL SMEARS

2020 ◽  
pp. 10-11
Author(s):  
Ankan Nandi ◽  
Debarshi Jana
Keyword(s):  
Observational Study ◽  
Body Cavity ◽  
Diagnostic Utility ◽  
Cytological Diagnosis ◽  
Cell Block ◽  
Tissue Architecture ◽  
Block Method ◽  
Exclusion Criteria ◽  
Cross Sectional ◽  
Malignant Effusions

Background: The cell block technique is one of the oldest method for evaluation of body cavity fluids and it increases the sensitivity of the diagnosis in comparison to conventional smear study. The main advantage of cell block technique is preservation of tissue architecture and obtaining multiple sections for special stains. Aim: To assess the utility of cell block method in increasing the sensitivity of cytological diagnosis of body cavity effusions as an adjunct to conventional smear method. Material and methods: It was institution based cross sectional observational study. All patients presenting with effusion and those who was give consent for participating in the study was included in this study. The study was conducted in Dept. Of Pathology, Nandigram Superspeciality Hospital. 100 cases fulfilling the predetermined inclusion and exclusion criteria was taken during the study period. Result: Association of Cellularity vs cell block was statistically significant (p<0.0001). Association of Architectural Pattern vs cell block was not statistically significant (p=0.7883). Association of cytological smears vs cell block was statistically significant (p=<0.0001). Conclusion: Cell block technique is superior to conventional smear technique, especially for malignant effusions.

DIAGNOSTIC UTILITY OF CELL BLOCK METHOD IN PLEURAL FLUID CYTOLOGY

2014 ◽  
Vol 1 (9) ◽  
pp. 1240-1245
Author(s):  
Gayathri M N ◽  
Kunal Puri ◽  
Satish M K ◽  
Ravikumar T ◽  
Bharathi M
Keyword(s):  
Pleural Fluid ◽  
Diagnostic Utility ◽  
Cell Block ◽  
Block Method ◽  
Fluid Cytology

scholarly journals Diagnostic utility of the cell block method versus the conventional smear study in pleural fluid cytology

2012 ◽  
Vol 29 (1) ◽  
pp. 11 ◽  
Author(s):  
Udasimath Shivakumarswamy ◽  
MaheshH Karigowdar ◽  
SurekhaU Arakeri ◽  
BR Yelikar
Keyword(s):  
Pleural Fluid ◽  
Diagnostic Utility ◽  
Cell Block ◽  
Block Method ◽  
Fluid Cytology

scholarly journals Limited Diagnostic Capacities of Two Commercial Assays for the Detection of Leptospira Immunoglobulin M Antibodies in Laos

2006 ◽  
Vol 13 (10) ◽  
pp. 1166-1169 ◽  
Author(s):  
Stuart D. Blacksell ◽  
Lee Smythe ◽  
Rattanaphone Phetsouvanh ◽  
Michael Dohnt ◽  
Rudy Hartskeerl ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Immunosorbent Assay ◽  
Gold Standard ◽  
Agglutination Test ◽  
Immunoglobulin M ◽  
Microscopic Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Diagnostic Utility

ABSTRACT The diagnostic utility of immunochromatographic (Leptotek) and enzyme-linked immunosorbent assay (ELISA; Panbio) tests for the detection of Leptospira immunoglobulin M antibodies was assessed in febrile adults admitted in Vientiane, Laos. Both tests demonstrated poor diagnostic accuracy using admission serum (Leptotek sensitivity of 47.3% and specificity of 75.5%: ELISA sensitivity of 60.9% and specificity of 65.6%) compared to the Leptospira “gold standard” microscopic agglutination test.

Cell Block in Cytological Diagnostics: Review of Preparatory Techniques

2018 ◽  
Vol 62 (4) ◽  
pp. 237-243 ◽  
Author(s):  
Leena Krogerus ◽  
Ivana Kholová
Keyword(s):  
Targeted Therapy ◽  
Study Design ◽  
Molecular Techniques ◽  
Cell Block ◽  
Wide Range ◽  
Blood Clots ◽  
The Future ◽  
Golden Standard ◽  
Agar Method ◽  
Cytological Diagnostics

Objective: The cell block (CB) technique refers to the processing of sediments, blood clots, or grossly visible tissue fragments from cytological specimens into paraffin blocks that can be cut and stained by the same methods used for histopathology. The technique brings additional tissue architectural information. CB can be used for ancillary techniques such as immunocytochemistry and molecular techniques. Study Design: We reviewed the literature on the various preparatory techniques of CBs. Results: There is a wide range of preparatory techniques for CBs and no golden standard for CBs exists: tens of methods are used in various institutions. The majority of the methods are modified in house techniques with a few commercially available kits. The techniques most commonly used are the plasma/thrombin method, the agar method, and commercially available Histogel- and Cellient CB-methods. Dissatisfaction with the cellular yield of the CBs is common. Conclusions: In the CBs, the cytological material is preserved for future use, which is a tremendous advantage in the era of targeted therapy and biobanking. The CB is thus central to the future of cytology: more can be done with less material and with less invasiveness to the patient.

The cell block method is useful for predicting the primary site of cancer in cases of peritonitis carcinomatosa

2021 ◽  
Vol 60 (2) ◽  
pp. 86-93
Author(s):  
Yoshitomo KONISHI ◽  
Satoko KAGABU ◽  
Iwao ONO ◽  
Akira ISHII ◽  
Hiroto KON ◽  
...  
Keyword(s):  
Primary Site ◽  
Cell Block ◽  
Block Method ◽  
Peritonitis Carcinomatosa

scholarly journals A Study of the Diagnostic Accuracy of an Existing Multivariable Test to Predict Shoulder Dystocia

2019 ◽  
Vol 09 (03) ◽  
pp. e262-e267
Author(s):  
Henry Alexander Easley ◽  
Todd Michael Beste
Keyword(s):  
Diagnostic Accuracy ◽  
Prediction Model ◽  
Study Design ◽  
False Positive ◽  
False Positive Rate ◽  
Shoulder Dystocia ◽  
The Other ◽  
Predictive Values ◽  
Positive Rate ◽  
Sensitivity Specificity

Objectives To evaluate the diagnostic accuracy of a multivariable prediction model, the Shoulder Screen (Perigen, Inc.), and compare it with the American College of Obstetricians and Gynecologists (ACOG) guidelines to prevent harm from shoulder dystocia. Study Design The model was applied to two groups of 199 patients each who delivered during a 4-year period. One group experienced shoulder dystocia and the other group delivered without shoulder dystocia. The model's accuracy was analyzed. The performance of the model was compared with the ACOG guideline. Results The sensitivity, specificity, positive, and negative predictive values of the model were 23.1, 99.5, 97.9, and 56.4%, respectively. The sensitivity of the ACOG guideline was 10.1%. The false-positive rate of the model was 0.5%. The accuracy of the model was 61.3%. Conclusion A multivariable prediction model can predict shoulder dystocia and is more accurate than ACOG guidelines.

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