scholarly journals Inheritance and linkage of allozymes in Juniperus phoenicea L. (Cupressaceae)

2014 ◽  
Vol 69 (3) ◽  
pp. 201-205 ◽  
Author(s):  
Andrzej Lewandowski ◽  
Joanna Samoćko ◽  
Adam Boratyński ◽  
Leon Mejnartowicz
Keyword(s):  
Mendelian Inheritance ◽  
Band Pattern ◽  
Men 1 ◽  
Enzyme Systems ◽  
Juniperus Phoenicea ◽  
Allozyme Loci ◽  
Linkage Relationships

Eight enzyme systems coding for 14 loci were assayed in <em>Juniperus phoenicea</em>. Mendelian inheritance was confirmed for allozymes at 10 loci (Fle 2, Gdh, Got 1, Idh, Mdh 1, Mdh 2, Men 1, Pgi, Pgm I, Pgm 2) by testing the fit of band-pattern segregation in macrogametophytes from heterozygous trees to the expected I: I ratio. Linkage relationships were examined for 21 pairs of allozyme loci and these pairs do not appear to be linked in our material.

scholarly journals Segregation and Linkage Relationships of Allozymes in Pinus Brutia Ten

2006 ◽  
Vol 55 (1-6) ◽  
pp. 155-160
Author(s):  
Nuray Kaya ◽  
K. Isik
Keyword(s):  
Seed Orchard ◽  
Mendelian Inheritance ◽  
Recombination Fraction ◽  
Starch Gel Electrophoresis ◽  
Mendelian Segregation ◽  
Pinus Brutia ◽  
Segregation Ratios ◽  
Starch Gel ◽  
Allozyme Loci ◽  
Linkage Relationships

Abstract Female gametophytes of Pinus brutia Ten. were analyzed to study Mendelian segregation and linkage among allozyme loci in a seed orchard composed of 28 clones. Isozyme variants of nine enzyme systems encoding 14 loci from megagametophytes were assayed by starch gel electrophoresis. While six of the 14 loci were monomorphic, the remaining eight were polymorphic. Analysis of observed segregation ratios of all polymorphic loci except for Sdh1, showed a good fit to the 1:1 ratios expected for Mendelian inheritance. Linkage relationships of the eight polymorphic allozyme loci were analyzed by testing 23 of the 28 possible two-locus combinations. Two-locus segregations in three pairs (Got2- Mdh1, Got2-Pgi2 and Pgd3-Sdh1) were significantly heterogeneous (P ≤ 0.05). Relatively weak linkage was observed between only one pair of genes (Adh2:Got2). Recombination fraction for Adh2:Got2 was found to be 0.24 (± 0.06).

scholarly journals 147 GENETIC DIVERSITY, AND INHERITANCE AND LINKAGE OF ISOZYME LOCI IN MELON (Cucumis melo L.)

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 449f-449 ◽  
Author(s):  
V. V. Meglic ◽  
T. F. Horejsi ◽  
J. E. Staub ◽  
J. D. McCreight
Keyword(s):  
Genetic Diversity ◽  
Cucumis Melo ◽  
Multivariate Analyses ◽  
Mendelian Inheritance ◽  
Linkage Groups ◽  
Plant Introductions ◽  
Enzyme Systems ◽  
Isozyme Loci ◽  
Cucumis Melo L ◽  
Linkage Relationships

The genetic diversity of 400 U.S. melon germplasm plant introductions was assessed using 35 enzyme systems. Polymorphisms were observed at 24 putative loci (Ac, Acp1, Acp4, Ak2, Ak3. Ak4, Fdp1, Fdp2, Fdp4, Gpi, Idh, Mdh2, Mdh4, Mdh5, Mdhb, Mpi1, Mpi2, Pgd1, Pgd2, Pgm, Pep-g1, Pep-1a, Pep-pap, Skdh) representing 17 different enzymes. Sixteen loci demonstrated simple Mendelian inheritance. Multivariate analyses aided in reduction of data using 16 loci and linkage relationships were observed among the plant introductions. Two of 16 loci (Pgd1 and Acp1) segregated independently. Fourteen loci were assigned into three linkage groups (A-C): A Fdp1, Fdp2, Acp4, Skdh; B Mdh2, Mdh4, Mdh5, Mdh6, Pep-g1, Pgm; C Mpi2, Ac, Idh.

Genetics of isozyme variants and linkage relationships among allozyme loci in 35 eastern white pine clones

1981 ◽  
Vol 11 (3) ◽  
pp. 573-579 ◽  
Author(s):  
R. T. Eckert ◽  
R. J. Joly ◽  
D. B. Neale
Keyword(s):  
Gel Electrophoresis ◽  
Direct Evidence ◽  
Evidence Based ◽  
Starch Gel Electrophoresis ◽  
White Pine ◽  
Chi Square ◽  
Enzyme Systems ◽  
Starch Gel ◽  
Allozyme Loci ◽  
Linkage Relationships

Megagametophyte tissue from seeds of 35 clones of Pinusstrobus L. were subjected to horizontal starch gel electrophoresis. The resulting gels were tested for activity of 10 enzyme systems. Isozymes observed were under control of at least 17 loci. Direct evidence, based on segregation of allozymes in megagametophytes of heterozygous clones, is presented for nine loci in five systems (LAP, GPI, GOT, PGM, and AP). Three polymorphic loci were detected for LAP. No genetic variation was detected for IDH, ADH, GLY-3-PD, GDH, or 6 PDG.Five pairs of linked loci were detected by chi-square analyses of joint segregation. Twenty-five of 36 possible two-locus combinations among 18 clones were evaluated. Recombination estimates suggest relatively strong linkage between LAP1-LAP2 and GPI2-GOT1; weaker linkage between LAP2-GPI2 (based on one clone only); and weak linkage between LAP2-AP1 and GOT3-AP1.

scholarly journals Linkage relationships of allozyme loci in Pinus sylvestris

Hereditas ◽  
2008 ◽  
Vol 111 (2) ◽  
pp. 91-97 ◽  
Author(s):  
ALFRED E. SZMIDT ◽  
OUTI MUONA
Keyword(s):  
Pinus Sylvestris ◽  
Allozyme Loci ◽  
Linkage Relationships

scholarly journals Inheritance of Phosphoglucoisomerase in Fountain Grass

1995 ◽  
Vol 120 (3) ◽  
pp. 543-547
Author(s):  
M. Hockenberry Meyer ◽  
Donald B. White
Keyword(s):  
Gel Electrophoresis ◽  
Growth Habit ◽  
Mendelian Inheritance ◽  
Starch Gel Electrophoresis ◽  
Enzyme Systems ◽  
Segregation Ratios ◽  
Starch Gel ◽  
Pennisetum Alopecuroides ◽  
Slow Band ◽  
Growth Habits

Starch gel electrophoresis was used to screen 10 enzyme systems for variation in fountain grass, Pennisetum alopecuroides (L.) Spreng. plants exhibiting four different growth habits: dwarf(d), mound(m), prostrate(p), and upright (u). Only phosphoglucoisomerase (PGI; E.C. 5.3.1.9) was found to be polymorphic at one locus, PGI-2, and was expressed as two alleles, which appeared to be associated with growth habit. The dwarf form expressed one slow band (SS), the mound and prostrate forms exhibited one fast band (FF), and the upright form carried triple bands indicating a heterodimer (FS). Hybrids between FF and SS parents were detected as triple bands (FS). Three generations of progeny resulting from 16 crosses and selfs of these growth habits all followed the expected segregation ratios for typical Mendelian inheritance of this isozyme.

Linkage of allozyme loci in Japanese red pine (Pinusdensiflora)

1993 ◽  
Vol 23 (4) ◽  
pp. 680-687 ◽  
Author(s):  
Mohammad Na'iem ◽  
Yoshihiko Tsumura ◽  
Kohji Uchida ◽  
Toru Nakamura ◽  
Kihachiro Ohba
Keyword(s):  
Information Criterion ◽  
Linkage Study ◽  
Red Pine ◽  
Linkage Groups ◽  
Chi Square ◽  
Point Mapping ◽  
Japanese Red Pine ◽  
Plus Tree ◽  
Allozyme Loci ◽  
Linkage Relationships

Linkage relationships among 29 polymorphic loci were investigated in Japanese red pine, Pinusdensiflora Sieb. et Zucc. Haploid megagametophytes from 12 highly heterozygous plus-tree clones were used. A total of 217 pairs of two-locus combinations were available for the linkage study. Chi-square and Akaike Information Criterion analyses identified 19 pairs of linkages. Close linkages (r = 0–0.10) were seen for Est-2, 3–Aap-2, 3, Dia-1–Acp, and Shd-1–Got-1. Strong linkages (r = 0.10–0.20) were seen for Est-2, 3–Dia-1, Est-2, 3–Acp, Aap-2, 3–Dia-1, Got-2–Lap-1, and Pgm–Aap-1. Moderate linkages (r = 0.20–0.30) were seen for Adh–Sod, Adh–Pgi-1, 2, Adh–Lap-1, G2d–G6p, and Dia-2–Amy pair of linkages. The remaining six pair combinations, namely 6Pg-1–Est-2, 3, 6Pg-1–Acp, Est-1–Acp, Est-2, 3–Shd-1, Sod–Pgi-1, 2, and Adh–Got-2 were weakly linked (r = 0.30–0.40). In all, 22 loci were classified into five linkage groups by means of three-point mapping methods.

scholarly journals A preliminary study of the allozyme variation in the grey hamster, Cricetulus migratorius (Mammalia: Rodentia), from the Asian part of Turkey

2011 ◽  
Vol 63 (2) ◽  
pp. 381-391 ◽  
Author(s):  
Osman İbiş ◽  
Coşkun Tez ◽  
Servet Özcan ◽  
Metin Kiliç ◽  
Murat Telcioğlu
Keyword(s):  
Genetic Variation ◽  
Gene Flow ◽  
Gel Electrophoresis ◽  
Allozyme Variation ◽  
Genetic Distances ◽  
Average Value ◽  
Enzyme Systems ◽  
The Mean ◽  
Preliminary Study ◽  
Allozyme Loci

The aim of the present study was to evaluate the degree of genetic variation and divergence by cellulose acetate gel electrophoresis between samples of Cricetulus migratorius, a cricetine rodent distributed in the Asian part (Anatolia) of Turkey. Out of twenty allozyme loci scored for fifteen enzyme systems, eleven loci were detected to be polymorphic in at least one locality of the Turkish C. migratorius. Indices of genetic variability (the percentage of polymorphic loci, mean number of alleles per locus, and mean observed and expected heterozygosities) were found to be P(95%) = 28, A = 1.3, Ho = 0.226 and He = 0.218, respectively. Nei?s unbiased genetic distances ranged from 0.000 to 0.153, with an average value of 0.069. The mean gene flow was calculated to be Nm = 0.7484. This is a preliminary study describing the allozymic variations of C. migratorius from Turkey. As there are no extensive data on the allozymic variations of C. migratorius from other regions, our results could not be compared, in detail with those of other populations of the species C. migratorius.

LINKAGE RELATIONSHIPS AMONG 19 POLYMORPHIC ALLOZYME LOCI IN COASTAL DOUGLAS-FIR (PSEUDOTSUGA MENZIESII var. MENZIESII)

1982 ◽  
Vol 24 (1) ◽  
pp. 101-108 ◽  
Author(s):  
Y. A. El-Kassaby ◽  
O. Sziklai ◽  
F. C. Yeh
Keyword(s):  
Pseudotsuga Menziesii ◽  
Genetic Linkage ◽  
Douglas Fir ◽  
Conifer Species ◽  
Polymorphic Loci ◽  
Allozyme Loci ◽  
Linkage Relationships

Linkage relationships among 19 different polymorphic loci in coastal Douglas-fir (Pseudotsuga menziesii var. menziesii [Mirb.] Franco) were studied. A total of 115 of 171 possible two-locus combinations were tested. Out of the 115 combinations, a total of 106 conform to the hypothesis of independence between loci indicating that no linkage exists between these pairs. Of the remaining nine pairs, two were tightly linked with recombination frequencies of 0.02 (Aat2:Pgi2) and 0.22 (Aat3:Sod). The remaining were loosely linked, with recombination frequencies varying between 0.33 and 0.42. Two of these nine linked pairs have been detected in other conifer species. It was not possible to study three-point linkages due to the lack of appropriate combinations. To our knowledge, these results provide the first demonstration of genetic linkage in Douglas-fir.

scholarly journals DIVERSITY, INHERITANCE, AND LINKAGE RELATIONSHIPS OF ALLOZYME CODING LOCI IN CUCUMBER (Cucumis sativus L.).

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1153e-1153 ◽  
Author(s):  
Larry D. Knerr ◽  
Jack E. Staub
Keyword(s):  
Genetic Diversity ◽  
Cucumis Sativus ◽  
Germplasm Collection ◽  
Genetic Distances ◽  
Linkage Groups ◽  
Cucumis Sativus L ◽  
Plant Introductions ◽  
Set Up ◽  
Allozyme Loci ◽  
Linkage Relationships

The available U. S. Cucumis sativus germplasm collection (754 Plant Introductions) was electrophoretically screened for genetic diversity using 39 enzymes representing a total of 57 loci. Polymorphisms were observed at 18 loci which included g2dh, gpi1, gpi2, gr1, gr2, idh, mdh1, mdh2, mdh3, mpi2, pep-la2, pep-pap2, per4, pgd1, pgd2, pgm1, pgm3, and skdh. Appropriate crosses were set up to verify the inheritance of and test linkages among these loci. Four allozyme linkage groups have currently been identified. Representative linkages and their genetic distances include: gpi1 - mdh3 (20); pgm1 - pgd1 (25); and g2dh - pgd2 (19). Additionally, crosses were made to marker stocks to test for linkages between some allozyme loci and loci coding for resistance to downy mildew and anthracnose, long hypocotyl, divided leaf, short petiole, glabrous, compact plant, determinate, little leaf, and bitter free (bi).

Sign in / Sign up

Export Citation Format

Share Document