LINKAGE RELATIONSHIPS AMONG 19 POLYMORPHIC ALLOZYME LOCI IN COASTAL DOUGLAS-FIR (PSEUDOTSUGA MENZIESII var. MENZIESII)
Linkage relationships among 19 different polymorphic loci in coastal Douglas-fir (Pseudotsuga menziesii var. menziesii [Mirb.] Franco) were studied. A total of 115 of 171 possible two-locus combinations were tested. Out of the 115 combinations, a total of 106 conform to the hypothesis of independence between loci indicating that no linkage exists between these pairs. Of the remaining nine pairs, two were tightly linked with recombination frequencies of 0.02 (Aat2:Pgi2) and 0.22 (Aat3:Sod). The remaining were loosely linked, with recombination frequencies varying between 0.33 and 0.42. Two of these nine linked pairs have been detected in other conifer species. It was not possible to study three-point linkages due to the lack of appropriate combinations. To our knowledge, these results provide the first demonstration of genetic linkage in Douglas-fir.