Genetics of isozyme variants and linkage relationships among allozyme loci in 35 eastern white pine clones

1981 ◽  
Vol 11 (3) ◽  
pp. 573-579 ◽  
Author(s):  
R. T. Eckert ◽  
R. J. Joly ◽  
D. B. Neale
Keyword(s):  
Gel Electrophoresis ◽  
Direct Evidence ◽  
Evidence Based ◽  
Starch Gel Electrophoresis ◽  
White Pine ◽  
Chi Square ◽  
Enzyme Systems ◽  
Starch Gel ◽  
Allozyme Loci ◽  
Linkage Relationships

Megagametophyte tissue from seeds of 35 clones of Pinusstrobus L. were subjected to horizontal starch gel electrophoresis. The resulting gels were tested for activity of 10 enzyme systems. Isozymes observed were under control of at least 17 loci. Direct evidence, based on segregation of allozymes in megagametophytes of heterozygous clones, is presented for nine loci in five systems (LAP, GPI, GOT, PGM, and AP). Three polymorphic loci were detected for LAP. No genetic variation was detected for IDH, ADH, GLY-3-PD, GDH, or 6 PDG.Five pairs of linked loci were detected by chi-square analyses of joint segregation. Twenty-five of 36 possible two-locus combinations among 18 clones were evaluated. Recombination estimates suggest relatively strong linkage between LAP1-LAP2 and GPI2-GOT1; weaker linkage between LAP2-GPI2 (based on one clone only); and weak linkage between LAP2-AP1 and GOT3-AP1.

scholarly journals Inheritance of ADH, 6-PGDH, PGM, and SKDH in Allium fistulosum L.

1994 ◽  
Vol 119 (2) ◽  
pp. 335-338 ◽  
Author(s):  
Paul D. Mangum ◽  
Ellen B. Peffley
Keyword(s):  
Alcohol Dehydrogenase ◽  
Gel Electrophoresis ◽  
Allium Fistulosum ◽  
Starch Gel Electrophoresis ◽  
Shikimate Dehydrogenase ◽  
Chi Square ◽  
Phosphogluconate Dehydrogenase ◽  
Enzyme Systems ◽  
Isozyme Loci ◽  
Starch Gel

Horizontal starch gel electrophoresis was used to study the inheritance of isozyme phenotypes of four enzyme systems [alcohol dehydrogenase (ADH), 6-phosphogluconate dehydrogenase (6-PGDH), phosphoglucomutase (PGM), and shikimate dehydrogenase (SKDH)] in Allium fistulosum L. by monitoring segregations in backcross and F2 progeny. Segregation for most of the polymorphisms fit the expected Mendelian ratios as tested by the chi-square statistic. Three new isozyme loci were defined for onion. Two loci were found for 6-PGDH. Locus one was dimeric with two alleles, and locus two was monomorphic. SKDH was monomeric with two alleles.

scholarly journals Inheritance and linkage relationships of allozyme variants of ilex paraguariensis St. Hil

2009 ◽  
Vol 52 (6) ◽  
pp. 1443-1451
Author(s):  
Simone Neumann Wendt ◽  
Valderês Aparecida de Sousa ◽  
Alexandre Magno Sebbenn ◽  
José Alfredo Sturion ◽  
Felipe Luíz ◽  
...  
Keyword(s):  
Linkage Disequilibrium ◽  
Segregation Distortion ◽  
Gel Electrophoresis ◽  
Ilex Paraguariensis ◽  
Starch Gel Electrophoresis ◽  
Polymorphic Loci ◽  
Gene Segregation ◽  
Enzyme Systems ◽  
Starch Gel ◽  
Linkage Relationships

Eighteen enzyme systems were examined in Ilex paraguariensis St. Hil. using starch gel electrophoresis. Seven out of 12 active isozyme systems revealed one or more polymorphic loci (PGI, GOT, MR, G-6PDH, MDH, NDH, and 6-PGDH). However, the segregation and linkage analyses were performed only for PGI, GOT, G-6PDH and 6-PGDH systems. Gene segregation at these loci was regular, except for a few trees that showed segregation distortion. Weak linkage disequilibrium between loci was detected, but it was not enough to influence the multilocus estimate.

scholarly journals INHERITANCE OF ISOZYMES: DESCRIPTION OF NEW LOCI IN ONIONS ISOZYMES

HortScience ◽  
1993 ◽  
Vol 28 (4) ◽  
pp. 269D-269
Author(s):  
Paul D. Mangum ◽  
Ellen B. Peffley
Keyword(s):  
Allium Cepa ◽  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Shikimate Dehydrogenase ◽  
Chi Square ◽  
Phosphogluconate Dehydrogenase ◽  
Enzyme Systems ◽  
Isozyme Loci ◽  
Starch Gel ◽  
Mode Of Inheritance

Horizontal starch gel electrophoresis was used to study the mode of inheritance of isozyme phenotypes of four enzyme systems (ADH, 6-PGDH, PGM, and SKDH) in Allium cepa L. and A. fistulosum L. by monitoring segregations in backcross and F2 progeny. Segregation for most of the polymorphisms fit the expected Mendelian ratios as tested by the chi-square statistic. Three new isozyme loci were defined for onion. 6-phosphogluconate dehydrogenase was dimeric in structure, with two alleles present at the first locus, while a second locus was monomorphic. Shikimate dehydrogenase was monomeric with two alleles.

Intraspecific genetic variability of isozymes in the ectomycorrhizal fungus Suillus tomentosus

1988 ◽  
Vol 66 (3) ◽  
pp. 588-594 ◽  
Author(s):  
Hong Zhu ◽  
Kenneth O. Higginbotham ◽  
Bruce P. Dancik ◽  
Stan Navratil
Keyword(s):  
Genetic Variability ◽  
Host Selection ◽  
Gel Electrophoresis ◽  
Genetic Similarity ◽  
Ectomycorrhizal Fungus ◽  
Habitat Isolation ◽  
Starch Gel Electrophoresis ◽  
Enzyme Systems ◽  
Forest Regions ◽  
Starch Gel

Mycelial extracts of 43 isolates of Suillus tomentosus (Kauffm.) Singer, Snell & Dick collected from four boreal forest regions in Alberta were subjected to starch gel electrophoresis. A total of 21 bands was resolved from eight different enzyme systems presumably representing 13 loci. Six loci were polymorphic among these isolates. Cluster and principal components analyses demonstrated that intraspecific genetic variability of this fungus existed among and within forest regions. Polymorphic loci of acid phosphatase and alkaline phosphatase exhibited the greatest genetic similarity among the isolates within forest regions. Habitat isolation and host selection could be the major sources of genetic variation among forest regions.

Isozyme Variation in Broom Snakeweed (Gutierrezia sarothrae)

Weed Science ◽  
1995 ◽  
Vol 43 (1) ◽  
pp. 156-162 ◽  
Author(s):  
Yanglin Hou ◽  
Tracy M. Sterling
Keyword(s):  
Cluster Analysis ◽  
New Mexico ◽  
Genetic Variability ◽  
Gel Electrophoresis ◽  
Isozyme Analysis ◽  
Starch Gel Electrophoresis ◽  
Enzyme Systems ◽  
Starch Gel ◽  
Morphological Differences ◽  
Gutierrezia Sarothrae

Broom snakeweed, a perennial rangeland shrub, is highly variable morphologically and can grow under a broad range of environmental conditions. In this study, isozyme analysis using starch gel electrophoresis was used to quantify genetic variability within and among New Mexico populations of broom snakeweed. Eight separate populations of broom snakeweed and one population of threadleaf snakeweed as a comparison were investigated. of the 10 enzyme systems examined, 16 loci were identified in eight populations and two species. Eleven loci were monomorphic in eight populations and two species and five loci were polymorphic in at least one population or species. Genetic variability was large in broom and threadleaf snakeweed populations as determined by isozyme analysis. Genetic variability among broom snakeweed populations was greater than that within populations for the five polymorphic loci. Cluster analysis of genetic distance and identity for the eight populations and two species characterized two major groups. Within broom snakeweed, cluster analysis characterized five groups. The two species shared most common alleles. The genetic variation identified in this research may account for the morphological differences and broad geographical distribution of broom snakeweed.

scholarly journals Inheritance of Phosphoglucoisomerase in Fountain Grass

1995 ◽  
Vol 120 (3) ◽  
pp. 543-547
Author(s):  
M. Hockenberry Meyer ◽  
Donald B. White
Keyword(s):  
Gel Electrophoresis ◽  
Growth Habit ◽  
Mendelian Inheritance ◽  
Starch Gel Electrophoresis ◽  
Enzyme Systems ◽  
Segregation Ratios ◽  
Starch Gel ◽  
Pennisetum Alopecuroides ◽  
Slow Band ◽  
Growth Habits

Starch gel electrophoresis was used to screen 10 enzyme systems for variation in fountain grass, Pennisetum alopecuroides (L.) Spreng. plants exhibiting four different growth habits: dwarf(d), mound(m), prostrate(p), and upright (u). Only phosphoglucoisomerase (PGI; E.C. 5.3.1.9) was found to be polymorphic at one locus, PGI-2, and was expressed as two alleles, which appeared to be associated with growth habit. The dwarf form expressed one slow band (SS), the mound and prostrate forms exhibited one fast band (FF), and the upright form carried triple bands indicating a heterodimer (FS). Hybrids between FF and SS parents were detected as triple bands (FS). Three generations of progeny resulting from 16 crosses and selfs of these growth habits all followed the expected segregation ratios for typical Mendelian inheritance of this isozyme.

scholarly journals Isozymes for Cultivar Identification in Kiwifruit

HortScience ◽  
1991 ◽  
Vol 26 (7) ◽  
pp. 899-902 ◽  
Author(s):  
R. Messina ◽  
R. Testolin ◽  
M. Morgante
Keyword(s):  
New Zealand ◽  
Genetic Markers ◽  
Gel Electrophoresis ◽  
Cultivar Identification ◽  
Starch Gel Electrophoresis ◽  
Banding Patterns ◽  
Discriminating Power ◽  
Enzyme Systems ◽  
Starch Gel ◽  
Simultaneous Comparison

The usefulness of isozyme banding patterns as genetic markers in kiwifruit [Actinidia deliciosa (A. Chev.) C.F. Liang et A.R. Ferguson] was investigated using starch gel electrophoresis. Fifty-four entries putatively belonging to seven female and two male kiwifruit cultivars were examined for 13 enzyme systems (AAT, ACO, GDH, G6PDH, IDH, MDH, ME, MNR, NDH, 6PGD, PGI, PGM, and SKDH). Four enzyme systems, ACO, MDH, NDH, and SKDH, showed identical banding patterns in all clones surveyed. Of the remaining enzymes, AAT, PGI, and PGM had the best discriminating power. Six enzyme systems (GDH, G6PDH, IDH, ME, MNR, and 6PGD), though showing polymorphic banding patterns, were poorly resolved. All the New Zealand cultivars were uniquely identified by the simultaneous comparison of the AAT, PGI, and PGM zymograms. Some enzyme systems were also polymorphic among plants within the same cultivar, thus proving the heterogeneity of kiwifruit material introduced into Europe in the early 1970s.

ELECTROPHORETIC ANALYSIS OF DIVERSITY WITHIN CUPRESSUS SEMPERVIRENS L. GROWING IN ISRAEL

1997 ◽  
Vol 45 (1) ◽  
pp. 1-8
Author(s):  
Gabriel Schiller ◽  
Leonid Korol
Keyword(s):  
Gel Electrophoresis ◽  
Gene Diversity ◽  
Electrophoretic Analysis ◽  
Starch Gel Electrophoresis ◽  
Cupressus Sempervirens ◽  
Single Tree ◽  
Enzyme Systems ◽  
High Gene ◽  
Starch Gel ◽  
Viable Seeds

Isoenzyme variants withinCupressus sempervirensL. planted in Israel have been identified using starch gel electrophoresis of enzymes extracted from the megagametophytic and perisperm seed tissue. Single-tree cone collection from 493 trees growing in 27 populations at holy sites and in plantations planted at the end of the 19th and the beginning of the 20th century in the country was done in 1986–1987. In 1994 viable seeds were available from only 267; of them, 140 trees were phenotypically identified as var.pyramidalisand 67 as being of var.horizontalis; the other 60 trees were not identified phenotypically. The results gained show that in the plantedC. sempervirensL. under investigation 11 (47.8%) out of the 23 gene loci in 13 enzyme systems analyzed were polymorphic; they contain a relatively high gene diversity of 0.479. The genetic distance between the two varieties planted in Israel is 0.007. Differences in allele frequencies between the two varieties occurred only in 5 loci, viz., IDH2PGI2, MDH3, PGM1, and ACO1.

scholarly journals Growth and isoenzyme comparison of five isolates of Venturia inaequalis

2005 ◽  
Vol 71 (2) ◽  
pp. 65-71 ◽  
Author(s):  
E. Roig ◽  
P. Neumann ◽  
J.-P. Simon
Keyword(s):  
Carbonic Anhydrase ◽  
Gel Electrophoresis ◽  
Venturia Inaequalis ◽  
Starch Gel Electrophoresis ◽  
Enzyme Systems ◽  
Race 1 ◽  
Starch Gel ◽  
Specific Proteins ◽  
Race 2 ◽  
Isoenzyme Patterns

Observation of the mycelial aspect of five isolates representing the fîve races of Venturia inaequalis indicates that it is possible to differentiate these isolates by their growth pattern and the isolate of race 3 appears to be the most easily distinguishable. Fifteen enzyme systems and non-specific proteins have been analyzed by polyacrylamide and starch gel electrophoresis. Thirteen of the isoenzymes systems and non-specific proteins showed no variation among the five isolates. Esterases isoenzyme patterns allow separation of the isolates since only one isoenzyme is common to all isolates and two others are shared by isolates of race 1 and 5. One carbonic anhydrase isoenzyme was observed to be specific to the isolate of race 2.

scholarly journals Isozyme Polymorphisms in Carob Cultivars

HortScience ◽  
1992 ◽  
Vol 27 (3) ◽  
pp. 257-258 ◽  
Author(s):  
J. Tous ◽  
C. Olarte ◽  
M.J. Truco ◽  
P. Arús
Keyword(s):  
Aspartate Aminotransferase ◽  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Ceratonia Siliqua ◽  
Leaf Extracts ◽  
Enzyme Systems ◽  
Starch Gel

The variability of isozymes in nine enzyme systems was studied in 25 carob (Ceratonia siliqua L.) cultivars using starch gel electrophoresis of leaf extracts. Five enzymes (phosphoglucomutase, phosphoglucoisomerase, aspartate aminotransferase, shikimic dehydrogenase, and aconitase) were polymorphic, making it possible for the 25 cultivars to be classified into eight phenotype categories.

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