Sensitivity Of Fluorescent Microscopy In Detecting Mycobacterium Leprae In Tissue Sections

10.5580/278b ◽  
2011 ◽  
Vol 11 (2) ◽  
Keyword(s):  
Fluorescent Microscopy ◽  
Mycobacterium Leprae ◽  
Tissue Sections

Role of fluorescent microscopy in detecting Mycobacterium leprae in tissue sections

2003 ◽  
Vol 7 (2) ◽  
pp. 78-81
Author(s):  
Sunil V. Nayak ◽  
A.S. Shivarudrappa ◽  
Ahmed Siddiq Mukkamil
Keyword(s):  
Fluorescent Microscopy ◽  
Mycobacterium Leprae ◽  
Tissue Sections

scholarly journals Molecular imaging can identify the location to perform a frozen biopsy during intraoperative frozen section consultation

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0252731
Author(s):  
Mitchell G. Bryski ◽  
Lydia G. Frenzel-Sulyok ◽  
E. James Delikatny ◽  
Charuhas Deshpande ◽  
Leslie A. Litzky ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Murine Model ◽  
Sampling Error ◽  
Frozen Section ◽  
Fluorescent Microscopy ◽  
Surgical Oncology ◽  
Tissue Samples ◽  
Surgical Specimen ◽  
Intraoperative Frozen Section ◽  
Tissue Sections

Background Intraoperative frozen section (FS) consultation is an important tool in surgical oncology that suffers from sampling error because the pathologist does not always know where to perform a biopsy of the surgical specimen. Intraoperative molecular imaging is a technology used in the OR to visualize lesions during surgery. We hypothesized that molecular imaging can address this pathology challenge in FS by visualizing the cancer cells in the specimen in the pathology suite. Here, we report the development and validation of a molecular-imaging capable cryostat called Smart-Cut. Methods A molecular imaging capable cryostat prototype was developed and tested using a murine model. Tumors grown in mice were targeted with a NIR contrast agent, indocyanine green (ICG), via tail vein injection. Tumors and adjacent normal tissue samples were frozen sectioned with Smart-Cut. Fluorescent sections and non-fluorescent sections were prepared for H&E and fluorescent microscopy. Fluorescent signal was quantified by tumor-to-background ratio (TBR). NIR fluorescence was tested in one patient enrolled in a clinical trial. Results The Smart-Cut prototype has a small footprint and fits well in the pathology suite. Fluorescence imaging with Smart-Cut identified cancerous tissue in the specimen in all 12 mice. No false positives or false negatives were seen, as confirmed by H&E. The mean TBR in Smart-Cut positive tissue sections was 6.8 (SD±3.8). In a clinical application in the pathology suite, NIR imaging identified two lesions in a pulmonary resection specimen, where traditional grossing only identified one. Conclusion Molecular imaging can be integrated into the pathology suite via the Smart-Cut device, and can detect cancer in frozen tissue sections using molecular imaging in a murine model.

Investigation of unmatured hematopoietic cell migration in a mouse model

2010 ◽  
Vol 5 (5) ◽  
pp. 585-589
Author(s):  
Gene Biziulevičienė ◽  
Vytautas Kašėta ◽  
Giedrė Ramanauskaitė ◽  
Aida Vaitkuvienė
Keyword(s):  
Bone Marrow ◽  
Cell Migration ◽  
Fluorescent Microscopy ◽  
Contact Hypersensitivity ◽  
Mouse Tissue ◽  
Paw Edema ◽  
Spleen Tissue ◽  
Tissue Sections ◽  
Time Point

AbstractThe objective of this work was to examine the migration of transplanted bone marrow hematopoietic lin− cell population using the BALB/c mouse contact hypersensitivity model in vivo and to determine the time point at which they reach the site of injury (paw edema) as well as other undamaged organs, such as liver and spleen. Female BALB/c mice with induced contact hypersensitivity reaction were intravenously injected with 1×106 cells/mouse lin− cells, labeled with PKH67. The presence of lin− stained cells in mouse tissue sections was evaluated by fluorescent microscopy. After one hour, the labeled cells were found in mice paw edema and liver, and after 4 hours in spleen tissue. Migrated hematopoietic lin− cells remained in liver tissue for 48 h, and in spleen and paw edema at least for 72 h. Migrated stained cells in untreated paw were not found. The results prove that bone marrow unmatured hematopoietic cells are first found in paw edema, where they participate in the inhibition of tissue inflammation; these cells subsequently migrate to the liver and are found in the spleen shortly afterwards.

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