Value of Fluorescent Techniques for Detecting Mycobacterium Leprae in Tissue Sections

1963 ◽  
Vol 2 (1) ◽  
pp. 11-13
Author(s):  
Anthony Betts ◽  
Alfred Hollander
Keyword(s):  
Mycobacterium Leprae ◽  
Tissue Sections

Role of fluorescent microscopy in detecting Mycobacterium leprae in tissue sections

2003 ◽  
Vol 7 (2) ◽  
pp. 78-81
Author(s):  
Sunil V. Nayak ◽  
A.S. Shivarudrappa ◽  
Ahmed Siddiq Mukkamil
Keyword(s):  
Fluorescent Microscopy ◽  
Mycobacterium Leprae ◽  
Tissue Sections

The Application of Protein A-Gold Immunocytochemistry to Studies of Protein Secretion

1985 ◽  
Vol 43 ◽  
pp. 426-429
Author(s):  
George H. Herbener ◽  
Antonio Nanci ◽  
Moise Bendayan
Keyword(s):  
Tissue Section ◽  
Colloidal Gold ◽  
Unit Area ◽  
Protein A ◽  
Extracellular Proteins ◽  
Gold Complex ◽  
Second Step ◽  
Igg Antibodies ◽  
Tissue Sections ◽  
Antigenic Sites

Protein A-gold immunocytochemistry is a two-step, post-embedding labeling procedure which may be applied to tissue sections to localize intra- and extracellular proteins. The key requisite for immunocytochemistry is the availability of the appropriate antibody to react in an immune response with the antigenic sites on the protein of interest. During the second step, protein A-gold complex is reacted with the antibody. This is a non- specific reaction in that protein A will combine with most IgG antibodies. The ‘label’ visualized in the electron microscope is colloidal gold. Since labeling is restricted to the surface of the tissue section and since colloidal gold is particulate, labeling density, i.e., the number of gold particles per unit area of tissue section, may be quantitated with ease and accuracy.

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