scholarly journals Detection of mycobacterium leprae in tissue sections using auramine O fluorescent stain versus modified fite-faraco: A comparative study

2016 ◽  
Vol 45 ◽  
pp. 388 ◽  
Author(s):  
K. Bhardwaj ◽  
S. Ghate ◽  
R. Dhurat
Keyword(s):  
Comparative Study ◽  
Mycobacterium Leprae ◽  
Tissue Sections ◽  
Auramine O ◽  
Fluorescent Stain

Papanicolaou induced fluorescence, Ziehl-Neelsen and Auramine O stains on lymph node fine needle aspiration biopsy specimens from children: A comparative study

2018 ◽  
Vol 46 (7) ◽  
pp. 589-592
Author(s):  
Malesiba Mampotoko Selepe ◽  
Abraham Christoffel van Wyk ◽  
Kim Gilberte Pauline Hoek ◽  
Yvonne Prince ◽  
Paul David van Helden ◽  
...  
Keyword(s):  
Lymph Node ◽  
Comparative Study ◽  
Fine Needle Aspiration ◽  
Fine Needle Aspiration Biopsy ◽  
Needle Aspiration ◽  
Aspiration Biopsy ◽  
Fine Needle ◽  
Biopsy Specimens ◽  
Auramine O ◽  
Needle Aspiration Biopsy

scholarly journals COMPARATIVE STUDY BETWEEN OPTIMAL AND REDUCED FORMALIN-BASED FIXATIVE FOR NORMAL AND PATHOLOGICAL TISSUES

2019 ◽  
pp. 119-122
Author(s):  
SAWSAN SALIH ALHAROON ◽  
NAWAL MUSTAFA ABDULLAH ◽  
NADA HASHIM MOHAMED
Keyword(s):  
Side Effects ◽  
Comparative Study ◽  
Routine Examination ◽  
Laboratory Worker ◽  
Tissue Samples ◽  
Tissue Sections ◽  
Processing Procedure ◽  
Hypotonic Saline ◽  
Harmful Effects ◽  
Pathological Tissues

Objectives: This study aims to use an alternative fixative compound with reduced concentration of formalin to minimize the exposure and related side effects. Methods: Human normal and pathological tissue samples of different organs were immediately immersed in a modified fixative containing formalin, glycerin, ethanol, and hypotonic saline with pH around 7. Two time schedules were used at 8 and 12 h. Tissue samples were undergone routine processing procedure used in histopathological laboratories, scoring of samples was done, tissue sections of new compound fixative were examined for cytoplasmic, nuclear, and architectural properties and compared with 10% neutral buffered formalin (NBF). Results: Tissue samples treated with modified fixative showed good and acceptable preservation of structural, cellular details with less harmful effects when compared with those preserved with routine 10% (NBF). Conclusion: The suitability and effectiveness of reduced formalin-based fixative in histological and histopathological routine examination with minimal harmful effects for laboratory worker were clearly indicated in this study.

scholarly journals Giemsa as a fluorescent stain for mineralized bone.

1994 ◽  
Vol 42 (5) ◽  
pp. 677-680 ◽  
Author(s):  
J N Bradbeer ◽  
M Riminucci ◽  
P Bianco
Keyword(s):  
Fluorescence Microscopy ◽  
Differential Staining ◽  
Elastic Fibers ◽  
Eosin Y ◽  
Fluorescent Staining ◽  
Tissue Sections ◽  
Confocal Fluorescence ◽  
Differential Binding ◽  
Fluorescent Stain ◽  
New Applications

We present evidence for a previously unrecognized differential staining effect of Giemsa solution in fluorescence microscopy. The effect consists of selective fluorescent staining of mineralized bone (and elastic fibers) in tissue sections and, like the classical Romanowsky effect, is based on the differential binding of Eosin Y to tissue structures in the presence of Azur II and Methylene Blue. This effect opens the way to new applications of the Giemsa solution in fluorescence microscopy and in confocal fluorescence microscopy.

A reliable fluorescent stain for fungi in tissue sections and clinical specimens

1984 ◽  
Vol 88 (2-3) ◽  
pp. 131-134 ◽  
Author(s):  
H. Holl�nder ◽  
W. Keilig ◽  
J. Bauer ◽  
E. Rothemund
Keyword(s):  
Clinical Specimens ◽  
Tissue Sections ◽  
Fluorescent Stain
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