Comparing the sensitivity of auramine-rhodamine fluorescence to polymerase chain reaction in the detection of Mycobacterium leprae in Fite-negative tissue sections

2017 ◽  
Vol 76 (5) ◽  
pp. 992-993 ◽  
Author(s):  
Dirk M. Elston ◽  
Maritza O. Liranzo ◽  
David M. Scollard
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycobacterium Leprae ◽  
Chain Reaction ◽  
Tissue Sections ◽  
Polymerase Chain

scholarly journals Detection of Mycobacterium leprae DNA by polymerase chain reaction in the blood of individuals, eight years after completion of anti-leprosy therapy

2001 ◽  
Vol 96 (8) ◽  
pp. 1123-1133 ◽  
Author(s):  
Adalberto Rezende Santos ◽  
Vivian Balassiano ◽  
Maria Leide W Oliveira ◽  
Marcia Aparecida da Silva Pereira ◽  
Patricia Barros Santos ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycobacterium Leprae ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Use of the polymerase chain reaction to detect Mycobacterium leprae in urine

2012 ◽  
Vol 45 (2) ◽  
pp. 153-157 ◽  
Author(s):  
K.R. Caleffi ◽  
R.D.C. Hirata ◽  
M.H. Hirata ◽  
E.R. Caleffi ◽  
V.L.D. Siqueira ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycobacterium Leprae ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Intracellular amplification of proviral DNA in tissue sections using the polymerase chain reaction.

1992 ◽  
Vol 40 (3) ◽  
pp. 333-341 ◽  
Author(s):  
K P Chiu ◽  
S H Cohen ◽  
D W Morris ◽  
G W Jordan
Keyword(s):  
Polymerase Chain Reaction ◽  
Cultured Cells ◽  
Chain Reaction ◽  
Tissue Sections ◽  
Mouse Mammary Tumor ◽  
Tumor Virus ◽  
Polymerase Chain ◽  
Thermal Cycler ◽  
Cellular Dna

We developed a new method to amplify cell DNA in situ using the polymerase chain reaction (PCR). Proviral sequences of mouse mammary tumor virus (MMTV) contained in cultured cells and tissue sections were amplified intracellularly using a thermal cycler. Two techniques were employed to maintain the localization of the amplified DNA. First, complementary tails at the 5' ends of the oligonucleotide primers resulted in the synthesis of high molecular weight concatamers containing the target sequences. Second, the PCR was carried out in a thin film of agarose solidified over the tissue sections. The specifically amplified and localized DNA was then detected by in situ hybridization (ISH). Our results demonstrate that (a) DNA in tissue sections can serve as the target for the polymerase chain reaction in situ, (b) cell morphology is maintained, and (c) a target of 167 BP can be specifically detected in individual cells. This technique should be generally applicable to amplifying cellular DNA targets in tissue sections for detection in situ.

The Use of a Specific DNA Probe and Polymerase Chain Reaction for the Detection of Mycobacterium leprae

1990 ◽  
Vol 162 (1) ◽  
pp. 193-200 ◽  
Author(s):  
D. L. Williams ◽  
T. P. Gillis ◽  
R. J. Booth ◽  
D. Looker ◽  
J. D. Watson
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycobacterium Leprae ◽  
Dna Probe ◽  
Chain Reaction ◽  
Polymerase Chain
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