scholarly journals An Assessment of Antibacterial Activity of Four Endodontic Sealers on Enterococcus faecalis by a Direct Contact Test: An In Vitro Study

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Lavanya Anumula ◽  
Swaroop Kumar ◽  
Venkata Suneel Kumar ◽  
Chandra Sekhar ◽  
Murali Krishna ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Enterococcus Faecalis ◽  
Direct Contact ◽  
Glass Ionomer ◽  
Polydimethyl Siloxane ◽  
Contact Test ◽  
Urethane Dimethacrylate ◽  
Endodontic Sealers ◽  
Direct Contact Test

Aim. To evaluate the antibacterial activity of four endodontic sealers on Enterococcus faecalis by a direct contact test. Material and Methods. Enterococcus faecalis was used as a test organism. Direct contact test which is based on measuring the effect of close contact between test bacteria and tested material on the kinetics of bacterial growth was performed to overcome the disadvantages of agar diffusion test. The sealers tested were zinc oxide eugenol-based sealer, glass-ionomer-based sealer, polydimethyl-siloxane-based sealer, and urethane dimethacrylate resin-based sealer. Data was collected by recording the optical density with the help of a spectrophotometer. Results. The sealers exhibited different inhibitory effects. The results obtained were subjected to statistical analysis by Kruskal Wallis analysis of variance and Dunn's multiple comparison test. Group comparison showed very highly significant difference between the groups. Conclusion. Zinc oxide eugenol-based sealer was the most effective and urethane dimethacrylate resin-based sealer was the least effective against Enterococcus faecalis, whereas glass-ionomer-based and polydimethyl-siloxane-based sealers were effective only for a short period. Inhibition of the bacterial growth is related to the direct contact of the microorganism with the sealer.

scholarly journals An assessment of antibacterial activity of three pulp capping materials on Enterococcus faecalis by a direct contact test: An in vitro study

2015 ◽  
Vol 09 (02) ◽  
pp. 240-245 ◽  
Author(s):  
Mine Koruyucu ◽  
Nursen Topcuoglu ◽  
E. Bahar Tuna ◽  
Sevda Ozel ◽  
Koray Gencay ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Enterococcus Faecalis ◽  
Direct Contact ◽  
In Vitro Study ◽  
Bacterial Density ◽  
Control Group ◽  
Pulp Capping ◽  
Contact Test ◽  
Direct Contact Test

ABSTRACT Objective: The aim of this in vitro study was to evaluate antimicrobial activities of three different pulp capping materials; Biodentine, mineral trioxide aggregate (MTA) Angelus, and Dycal against Enterococcus faecalis and their durability with time. Materials and Methods: Direct contact test was used for the assessment. Three sets of sealers were mixed and placed on microtiter plate wells: One set was used within 20 min of recommended setting time while others were used after 24-h and 1-week. E. faecalis suspension was placed directly on the materials for 1 h and then transferred to another plate with fresh media. Nine wells of bacteria without the tested cements served as the positive control. One well of the tested cements without bacteria served as the negative control. Bacterial growth was evaluated by a temperature-controlled microplate spectrophotometer for 1-h intervals among 24 h. Data were analyzed using Kruskal–Wallis test. Results: All tested materials showed less bacterial density than the control group. MTA, Biodentine, and Dycal showed significantly higher bacterial density than the control group in freshly mixed samples (P < 0.05). And MTA showed significantly higher antibacterial activity than Dycal (P < 0.05). In 24 h, materials did not show any differences (P > 0.05). MTA and Biodentine samples showed significant differences than Dycal; MTA also showed higher antibacterial activity than control in 1-week samples (P < 0.05). Conclusion: While freshly mixed MTA showed the best antibacterial activity over time, Biodentine had shown similar antibacterial activity to MTA.

Comparative evaluation of the antimicrobial efficacy of three endodontic sealers against Enterococcus faecalis with and without incorporation of Chitosan nanoparticles

2019 ◽  
Vol 11 (1) ◽  
pp. 21-28
Author(s):  
Dr. Rajisha K R
Keyword(s):  
Growth Inhibition ◽  
Enterococcus Faecalis ◽  
Direct Contact ◽  
Antibacterial Effect ◽  
Chitosan Nanoparticles ◽  
Contact Test ◽  
Agar Diffusion Test ◽  
Ah Plus ◽  
Endodontic Sealers ◽  
Direct Contact Test

Aims and Objectives: The aim of the present study was to evaluate and to compare the antibacterial activity of AH Plus, Tubliseal and MTA Fillapex against E.faecalis with and without incorporation of Chitosan nanoparticles. Methodology: The antibacterial effect against Enterococcus faecalis was evaluated by i) direct contact test (DCT) and ii) agar diffusion test (ADT). The nanoparticles were incorporated into the sealers at concentration of 2 % weight/ weight. Bacterial growth rates were analysed using ANOVA followed by Fischer Exact Test Results: The antibacterial tests demonstrated significant E.faecalis growth inhibition in the DCT with the sealer samples incorporated with chitosan nanoparticles. Direct contact test showed total growth inhibition of up to 8 logs in count in AH Plus, up to 7 logs in count in Tubliseal, and up to 6 logs in count in MTA Fillapex which were incorporated with chitosan nanoparticles. Significant differences were found between the unmodified sealers and the modified sealers. No antibacterial effect was observed in the ADT, indicating Chitosan nanoparticles were not diffusing into the agar. Conclusion: The endodontic sealers incorporating Chitosan nanoparticles exhibited significant and stable antimicrobial properties.

scholarly journals Low-temperature flow-synthesis-assisted urethane-grafted zinc oxide-based dental composites: physical, mechanical, and antibacterial responses

2021 ◽  
Vol 32 (8) ◽  
Author(s):  
Jaffar Hussain Bukhari ◽  
Abdul Samad Khan ◽  
Kashif Ijaz ◽  
Shahreen Zahid ◽  
Aqif Anwar Chaudhry ◽  
...  
Keyword(s):  
Compressive Strength ◽  
Zinc Oxide ◽  
Low Temperature ◽  
Abrasive Wear ◽  
Direct Contact ◽  
Dental Composites ◽  
Flow Synthesis ◽  
Contact Test ◽  
Experimental Novel ◽  
Direct Contact Test

AbstractA novel way was adopted to graft zinc oxide (ZnO) with urethane-modified dimethacrylate (UDMA) in order to utilize them as reinforcing agents in resin-based dental composites. Experimental novel composites were synthesized having UDMA-grafted and nongrafted ZnO, at a concentration of 0 wt.%, 5 wt.%, and 10 wt.%. The same concentrations of ZnO were also incorporated in Filtek Z250 XT (3 M ESPE, USA). The antibacterial behavior was evaluated against Streptococcus mutans by direct-contact test at one, three, and seven days of incubation. The compressive strength and Vickers microhardness were tested as per ISO 9917 and ISO/CD6507-1, respectively. For abrasive wear resistance, mass loss and roughness average after tooth-brushing cycles of 24,000 at custom-made tooth-brushing simulator were evaluated using noncontact profilometer. Data analysis was carried out using post hoc Tucky’s test and nonparametric Kruskal–Wallis test. Direct contact test revealed that the antibacterial potential of novel and commercial composites was increased with an increase in the concentration of grafted ZnO as compared with nongrafted, whereby the potential was the highest at day seven. There was a significant decrease in compressive strength and Vickers hardness of commercial composites on addition of grafted ZnO while there was no significant difference in the strength of experimental novel composite. The abrasive wear of commercial and experimental composites was within clinical limits. Low-temperature flow-synthesis method was successfully employed to synthesize grafted and nongrafted ZnO. The UDMA-grafted ZnO can be incorporated into dental composites without decreasing their strength and these composites can be used to combat secondary caries.

scholarly journals ANTIMICROBIAL ACTIVITY OF MINERAL TRIOXIDE AGGREGATE AND CALCIUM HYDROXIDE SEALER ON ENTEROCOCCUS FAECALIS STRAIN ATCC29212

2019 ◽  
pp. 123-125 ◽  
Author(s):  
TARA PRATHITA ◽  
NILA KESUMA DJAUHARIE ◽  
RATNA MEIDYAWATI
Keyword(s):  
Antimicrobial Activity ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Direct Contact ◽  
Mineral Trioxide Aggregate ◽  
Antimicrobial Effect ◽  
Time Points ◽  
Contact Test ◽  
Direct Contact Test

Objective: The objective of this study was to evaluate the antimicrobial activity of mineral trioxide aggregate (MTA) and calcium hydroxide (Ca(OH)2)sealers on Enterococcus faecalis at various time points after the preparation of the sealer.Methods: Antimicrobial activity of MTA and Ca(OH)2 sealer on E. faecalis (ATCC 29212) was assessed by the direct contact test immediately, 1 day,and 7 days after the preparation of the sealer.Results: The antimicrobial effect of MTA sealer was greatest 1 and 7 days after the preparation of the sealer. The effect of Ca(OH)2 was greatest 1 dayafter the preparation of the sealer.Conclusion: MTA sealer showed constant antimicrobial activity on E. faecalis overtime. By contrast, Ca(OH)2 sealer showed decreasing antimicrobialactivity overtime, with the greatest antimicrobial activity being 1 day after the preparation of the sealer.

scholarly journals Antimicrobial Activity and pH of Calcium Hydroxide and Zinc Oxide Nanoparticles Intracanal Medication and Association with Chlorhexidine

2015 ◽  
Vol 16 (8) ◽  
pp. 624-629 ◽  
Author(s):  
Mario Tanomaru-Filho ◽  
Juliane M Guerreiro-Tanomaru ◽  
Gisele Faria ◽  
Alana Souza Aguiar ◽  
Renato Toledo Leonardo
Keyword(s):  
Antimicrobial Activity ◽  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Calcium Hydroxide ◽  
Zinc Oxide Nanoparticles ◽  
Direct Contact ◽  
Oxide Nanoparticles ◽  
Diffusion Test ◽  
Agar Diffusion Test ◽  
Direct Contact Test

ABSTRACT Aim To evaluate pH and antibacterial activity of pastes with calcium hydroxide [Ca(OH)2] and zinc oxide (ZnO) microparticles (micro) or nanoparticles (nano) and association with 0.4% chlorhexidine against Enterococcus faecalis. Materials and methods The following pastes were analyzed: Ca(OH)2/ZnO micro, (2) Ca(OH)2/ZnO nano, (3) Ca(OH)2/ ZnO micro + 0.4% chlorhexidine, (4) Ca(OH)2/ZnO nano + 0.4% chlorhexidine. Antibacterial activity against E. faecalis was evaluated by agar diffusion test. The direct contact test on planktonic cells of E. faecalis was performed for 30 and 60 seconds. Root canals from bovine teeth were filled with the pastes and pH was evaluated after 1, 7, 14, 21, 30 and 60 days. The data obtained were submitted to the statistical tests analysis of variance (ANOVA) and Tukey or Kruskal-Wallis and Dunn test, with a 5% significance level. Results Calcium hydroxide and zinc oxide nano, and the pastes with 0.4% chlorhexidine were more effective in agar diffusion test. In the direct contact test, the pastes with chlorhexidine showed the highest effect after 30 seconds. All pastes eliminated E. faecalis after 60 seconds. All pastes promoted an increase in pH. The highest increase in pH was observed with nanoparticle medications after 1 and 7 days (p < 0.05). After this period, the pastes presented similar pH increase. Conclusion It was concluded that calcium hydroxide and zinc oxide nanoparticles promoted greater initial alkalinization. The antimicrobial activity of the pastes against E. faecalis is favored by the association with chlorhexidine. Clinical significance Although nanoparticles of calcium hydroxide and zinc oxide promoted antibacterial effect, the activity against E. faecalis is favored by association with chlorhexidine. How to cite this article Aguiar AS, Guerreiro-Tanomaru JM, Faria G, Leonardo RT, Tanomaru-Filho M. Antimicrobial Activity and pH of Calcium Hydroxide and Zinc Oxide Nanoparticles Intracanal Medication and Association with Chlorhexidine. J Contemp Dent Pract 2015;16(8):624-629.

scholarly journals In VitroEvaluation of the Antimicrobial Efficacy of Four Endodontic Biomaterials againstEnterococcus faecalis,Candida albicans, andStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Duddi Narendra Nirupama ◽  
Mohan Thomas Nainan ◽  
Rajendran Ramaswamy ◽  
Sethumadhavan Muralidharan ◽  
Hulimangala Hosakote Lingareddy Usha ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Direct Contact ◽  
Microbial Growth ◽  
Antimicrobial Property ◽  
Recurrent Infection ◽  
Antimicrobial Effect ◽  
Ah Plus ◽  
Endodontic Sealers ◽  
Root Canal Sealers ◽  
Direct Contact Test

Root canal sealers that possess good antimicrobial property can prevent residual and recurrent infection and contribute to successful endodontic therapy. This study evaluated the antimicrobial activity of four endodontic sealers, AH Plus, Tubliseal EWT, EndoRez, and iRoot SP, against three different microorganisms,E. faecalis, C. albicans, andS. aureus, by direct contact test. 10 μL microbial suspensions were allowed to directly contact the four endodontic sealers for 1 hr at 37°C. Subsequently microbial growth was measured spectrophotometrically every 30 min for 18 hours. The microbial suspensions were simultaneously tested to determine the antimicrobial effect of components which are capable of diffusing into the medium. The results revealed that AH Plus and iRootSP had significantly higher antimicrobial activity againstE. faecalis. AH Plus and Tubliseal EWT showed significantly higher antimicrobial activity againstC. albicansandS. aureuscompared to iRoot SP and EndoRez. EndoRez showed the least antimicrobial activity against all the three microorganisms. Inhibition of microbial growth is related to the direct contact of microorganisms with the sealers. In conclusion AH Plus had significantly higher antimicrobial activity againstE. faecalis, C. albicans, andS. aureus.

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